中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2011年
5期
261-264
,共4页
张锦程%傅敏%张陆弟%赵晖%魏小二
張錦程%傅敏%張陸弟%趙暉%魏小二
장금정%부민%장륙제%조휘%위소이
乙醇%急性脊髓型减压病%磁共振成像%脊髓诱发电位%病理组织学
乙醇%急性脊髓型減壓病%磁共振成像%脊髓誘髮電位%病理組織學
을순%급성척수형감압병%자공진성상%척수유발전위%병리조직학
Alcohol%Acute spinal cord decompression sickness%Magnetic resonance imaging%Spinal cord evoked potential%Histopathology
目的 观察乙醇治疗大白兔急性脊髓型减压病的疗效.方法 将58只新西兰大白兔分为乙醇组25只、生理盐水组20只和不采取任何治疗措施的对照组13只.将乙醇组15只、生理盐水组8只、对照组8只兔分别放入动物舱内,在15 min内用压缩空气加压至0.6 MPa,停留60 min,然后用5min匀速减压至常压出舱,制成急性脊髓型减压病模型.乙醇组出舱后30 min用25％乙醇溶液(3ml/ks),由耳缘静脉缓慢注射入血管；生理盐水组注射(3 ml/kg)生理盐水；对照组不作任何处理.所有兔均在造模前和出舱3d后行Tarlov法评估后肢运动、做MRI及测量脊髓诱发电位,随后处死,解剖,观察腹腔脏器并取胸腰段脊髓行HE染色和光镜检.结果 (1)Tarlov评分:乙醇组(4.31±0.63)分,生理盐水组( 1.25±0.50)分(与乙醇组比较,P＜0.01)；对照组(1.20±0.83)分(与乙醇组比较,P＜0.01).(2)MRI检查:乙醇组胸腰脊髓轻度肿胀,生理盐水组和对照组胸腰段脊髓肿胀,正常形态消失,并可见散在局灶性和弥漫性T2W高信号影.(3)SCEP检测:乙醇组的脊髓诱发电位N21潜伏期和波幅无明显变化,生理盐水组和对照组的脊髓诱发电位N21潜伏期较进舱前明显延长,差异有统计学意义(P＜0.01),波幅明显降低,差异有统计学意义(P＜0.01).(4)HE染色后,乙醇组光镜下仅见脊髓内少量散在出血及炎症细胞浸润；生理盐水组和对照组解剖可见尿潴留、肠胀气,光镜下可见脊髓弥漫出血、淤血、大量空泡及炎症细胞浸润.结论 乙醇有可能成为治疗急性脊髓型减压病的一种应急方法.
目的 觀察乙醇治療大白兔急性脊髓型減壓病的療效.方法 將58隻新西蘭大白兔分為乙醇組25隻、生理鹽水組20隻和不採取任何治療措施的對照組13隻.將乙醇組15隻、生理鹽水組8隻、對照組8隻兔分彆放入動物艙內,在15 min內用壓縮空氣加壓至0.6 MPa,停留60 min,然後用5min勻速減壓至常壓齣艙,製成急性脊髓型減壓病模型.乙醇組齣艙後30 min用25％乙醇溶液(3ml/ks),由耳緣靜脈緩慢註射入血管；生理鹽水組註射(3 ml/kg)生理鹽水；對照組不作任何處理.所有兔均在造模前和齣艙3d後行Tarlov法評估後肢運動、做MRI及測量脊髓誘髮電位,隨後處死,解剖,觀察腹腔髒器併取胸腰段脊髓行HE染色和光鏡檢.結果 (1)Tarlov評分:乙醇組(4.31±0.63)分,生理鹽水組( 1.25±0.50)分(與乙醇組比較,P＜0.01)；對照組(1.20±0.83)分(與乙醇組比較,P＜0.01).(2)MRI檢查:乙醇組胸腰脊髓輕度腫脹,生理鹽水組和對照組胸腰段脊髓腫脹,正常形態消失,併可見散在跼竈性和瀰漫性T2W高信號影.(3)SCEP檢測:乙醇組的脊髓誘髮電位N21潛伏期和波幅無明顯變化,生理鹽水組和對照組的脊髓誘髮電位N21潛伏期較進艙前明顯延長,差異有統計學意義(P＜0.01),波幅明顯降低,差異有統計學意義(P＜0.01).(4)HE染色後,乙醇組光鏡下僅見脊髓內少量散在齣血及炎癥細胞浸潤；生理鹽水組和對照組解剖可見尿潴留、腸脹氣,光鏡下可見脊髓瀰漫齣血、淤血、大量空泡及炎癥細胞浸潤.結論 乙醇有可能成為治療急性脊髓型減壓病的一種應急方法.
목적 관찰을순치료대백토급성척수형감압병적료효.방법 장58지신서란대백토분위을순조25지、생리염수조20지화불채취임하치료조시적대조조13지.장을순조15지、생리염수조8지、대조조8지토분별방입동물창내,재15 min내용압축공기가압지0.6 MPa,정류60 min,연후용5min균속감압지상압출창,제성급성척수형감압병모형.을순조출창후30 min용25％을순용액(3ml/ks),유이연정맥완만주사입혈관；생리염수조주사(3 ml/kg)생리염수；대조조불작임하처리.소유토균재조모전화출창3d후행Tarlov법평고후지운동、주MRI급측량척수유발전위,수후처사,해부,관찰복강장기병취흉요단척수행HE염색화광경검.결과 (1)Tarlov평분:을순조(4.31±0.63)분,생리염수조( 1.25±0.50)분(여을순조비교,P＜0.01)；대조조(1.20±0.83)분(여을순조비교,P＜0.01).(2)MRI검사:을순조흉요척수경도종창,생리염수조화대조조흉요단척수종창,정상형태소실,병가견산재국조성화미만성T2W고신호영.(3)SCEP검측:을순조적척수유발전위N21잠복기화파폭무명현변화,생리염수조화대조조적척수유발전위N21잠복기교진창전명현연장,차이유통계학의의(P＜0.01),파폭명현강저,차이유통계학의의(P＜0.01).(4)HE염색후,을순조광경하부견척수내소량산재출혈급염증세포침윤；생리염수조화대조조해부가견뇨저류、장창기,광경하가견척수미만출혈、어혈、대량공포급염증세포침윤.결론 을순유가능성위치료급성척수형감압병적일충응급방법.
Objective To investigate the therapeutic effect of alcohol on the treatment of acute spinal cord decompression sickness in rabbits.Methods Fifty-eight healthy New Zealand rabbits were randomly divided into 3 groups:the alcohol group ( group A,n =25 ),the saline solution group ( group B,n =20) and the control group ( group C,n =13).Rabbits in the animal chamber were pressurized with compressed air to a pressure of 0.6 MPa in 15 min,stayed at the said pressure for 60 min,then,were decompressed to atmospheric pressure with linear speed in 5 min.The model of acute spinal cord decompression sickness was successfully developed in 31 animals,of which 15 animals were in group A,8 animals in group B and another 8 animals in group C.The rabbits in group A were injected 25％ alcoholic solution (3 ml/kg) through the ear vein 30 min after decompression ; the animals in group B were treated with 3 ml/kg normal saline instead,while the rabbits in group C were left without any treatment.Spinal cord evoked potential (SCEP),magnetic resonance imaging (MRI) and Tarlov behavior scores were measured to assess motor neurologic function before compression and 3 days after decompression.All the animals were sacrificed,samples of spiral cord were taken from the animals of each group and HE staining was made for light microscopy.Results ( 1 ) Tarlov behavior scores:group A,4.31 ± 0.63 ; B group,1.25 ± 0.50 ( compared with group A,P ＜ 0.01 ) ; group C,1.20 ± 0.83 ( compared with group A,P ＜0.01 ).(2) MRI:animals in group A displayed mild swelling in thoracolumbar cord; animals in group B and C displayed moderate swelling,with normal morphology of spinal cord disappeared below the level of thoracolumbar cord and focal and diffuse hyper-intensity could be seen in the spinal cord on T2W images.(3) SCEP:for group A,no significant changes could be noted in the latency and amplitude of N21,3 days after decompression; for group B and group C,the latency of N21 delayed obviously 3 days after decompression,when it was compared with that before compression( P ＜ 0.01 ),and the amplitude of N21 also decreased obviously (P ＜0.01 ).(4) Group A:under the light microscope,HE staining revealed that there were only little sporadic hemorrhage and inflammatory infiltration in the spinal cord.Group B and group C:urine retention and intestinal tympanites could be observed,in addition,under the light microscope,local haemorrhage,extensive congestion and inflammatory cell infiltration could be observed in the spinal cord.Conclusions Alcohol treatment seemed to be a quick and effective method for the treatment of acute spinal cord decompression sickness.
