CAJ | 학술논문

目的评价新型多级结构仿生型关节软骨-骨一体化修复体的生物相容性,使用一体化修复体对兔关节全层软骨缺损进行修复,并对修复结果进行组织形态学观察.方法 1.生物相容性实验:包括急性毒性实验、溶血实验、免疫原性实验及慢性毒性实验.2.关节软骨修复实验:制作全层关节软骨缺损动物模型,随机于一侧植入一体化修复体,另一侧不予处理.术后4、6、8和12周分别处死动物,取修复组织行大体、放射学、组织学观察并用Wakitani法进行组织形态学评分.结果 1.生物相容性实验:(1)急性全身毒性实验动物体质量呈上升趋势,且各组体质量增加比较无显著性差异.(2)3种浓度梯度的修复材料溶血率均未超过5%.(3)慢性毒性实验:术后12周动物肝肾功能与正常对照组及术前比较无显著性差异.2.关节软骨修复实验:术后4～8周植入侧修复组织主要为透明软骨,表面光整平滑有光泽,与周围组织整合良好,对照侧无明显修复组织.Wakitani评分各组间差异均有统计学意义,实验组明显优于对照组.结论多级结构仿生型关节软骨-骨一体化修复体具有良好的生物相容性,并且在动物体内可诱导全层关节软骨缺损后的修复.
목적 평개신형다급결구방생형관절연골-골일체화수복체적생물상용성,사용일체화수복체대토관절전층연골결손진행수복,병대수복결과진행조직형태학관찰.방법 1.생물상용성실험:포괄급성독성실험、용혈실험、면역원성실험급만성독성실험.2.관절연골수복실험:제작전층관절연골결손동물모형,수궤우일측식입일체화수복체,령일측불여처리.술후4、6、8화12주분별처사동물,취수복조직행대체、방사학、조직학관찰병용Wakitani법진행조직형태학평분.결과 1.생물상용성실험:(1)급성전신독성실험동물체질량정상승추세,차각조체질량증가비교무현저성차이.(2)3충농도제도적수복재료용혈솔균미초과5%.(3)만성독성실험:술후12주동물간신공능여정상대조조급술전비교무현저성차이.2.관절연골수복실험:술후4～8주식입측수복조직주요위투명연골,표면광정평활유광택,여주위조직정합량호,대조측무명현수복조직.Wakitani평분각조간차이균유통계학의의,실험조명현우우대조조.결론 다급결구방생형관절연골-골일체화수복체구유량호적생물상용성,병차재동물체내가유도전층관절연골결손후적수복.
Objective To observe the biocompatibility of a biomimetic designing of a multi-grade compositions in repairing articular cartilage and subchondral bone in animal bodies and repair the fullthickness defects in articular cartilage with the compositions and to study the regenerated cartilage histomorphologically. Methods Biocompatibility study: Acute general toxicity test, Haemolysis test, subcutaneous implantation test and chronic toxicity test. Articular cartilage defects repaired experimental study :The models of defects in articular cartilage were made artificially in both condylus lateralis femoris of mature rabbits. Implanted with the biomimetic designing of a multi grade compositions randomly at one side as the experimental group and the other side were untreated as the control group. The rabbits were killed at 4, 6, 8and 12 weeks after operation, respectively, with 6 ones at each time, and the macroscopic, histological, ultrastroctural examinations and semi-quantity cartilage scoring employing Wakitanifa repaired cartilage value system were performed. Results Biocompatibility study: (1) The rabbits' weight in experimental group kept growing .(2) Haemolysis rate of rats to different concentrations of diffusion solution was＜5%.(3) In chronic toxic reaction, rabbits' liver and kidney function was not different compared with the control groups at 12weeks and the index before operation. Articular cartilage defects repaired experimental study: 4-8 weeks after operation, the defects in the experimental group were partly filled with hyaline cartilage. Twelve weeks after operation, the defects in the experimental group were completely filled with mature hyaline cartilage.However, fibrous tissues were seen in the control group all the time. At 4, 6, 8, and 12 weeks postoperatively, the Wakitanifa cartilage scores were (7.60±0.98), (5.69±0.58), (4.46±0.85) and (4.35±0.12), respectively,in the experimental group and (10.25±1.05), (9.04±0.96), (8.96±0.88) and (8.88±0.68), respectively, in the control group. Differences between the control group and the experimental group were significant. Conclu sion The biomimetic designing of a multi-grade compositions has good biocompatibility and may induce cartilage regeneration to repair the full-hickness defects of articular cartilage.