中华创伤杂志
中華創傷雜誌
중화창상잡지
Chinese Journal of Traumatology
2010年
10期
914-918
,共5页
王威%王岩%杨静%彭江%赵钧如
王威%王巖%楊靜%彭江%趙鈞如
왕위%왕암%양정%팽강%조균여
挤压综合征%模型,动物%挤压伤
擠壓綜閤徵%模型,動物%擠壓傷
제압종합정%모형,동물%제압상
Crush syndrome%Models,animal%Crush injury
目的 研究压迫面积、压迫时间和压迫重量与局部损伤及全身病理生理反应的相关性,初步摸索可重复的挤压综合征大鼠模型. 方法 清洁级雄性SD大鼠72只,按随机数字表法分为18个小组(每组4只大鼠).将压迫面积(单、双侧后肢)、压迫重量(2,3,4 kg)和压迫时间(4,6,8 h)进行排列组合后形成18种压迫条件.所有大鼠在压迫开始前和解压后3 h测量受压后肢周径,同时取血检测K+、CK、Cr和BUN,并在实验过程中观察肌红蛋白尿的发生率;解压后24 h取其受压局部肌肉及肾脏进行病理切片检查. 结果 所有大鼠解压3 h后受压肢体均明显肿胀(P＜0.05).各组中K+、CK、Cr和BUN均随压迫面积、时间及重量的增加和延长而明显上升(P＜0.05).局部受压肌肉呈明显水肿和坏死表现;肾脏出现肾小球、肾小管充血,压迫重量≥3 kg、压迫时间≥6 h组可见肾小管坏死及管型. 结论压迫时间、重量及面积的增加和延长可增加挤压伤局部损伤和全身反应的严重程度.受压面积为双侧后肢、压迫重量≥3 kg、压迫时间≥6 h可较为稳定地造成大鼠典型的挤压综合征表现,可初步作为建立挤压综合征大鼠模型的实验条件.
目的 研究壓迫麵積、壓迫時間和壓迫重量與跼部損傷及全身病理生理反應的相關性,初步摸索可重複的擠壓綜閤徵大鼠模型. 方法 清潔級雄性SD大鼠72隻,按隨機數字錶法分為18箇小組(每組4隻大鼠).將壓迫麵積(單、雙側後肢)、壓迫重量(2,3,4 kg)和壓迫時間(4,6,8 h)進行排列組閤後形成18種壓迫條件.所有大鼠在壓迫開始前和解壓後3 h測量受壓後肢週徑,同時取血檢測K+、CK、Cr和BUN,併在實驗過程中觀察肌紅蛋白尿的髮生率;解壓後24 h取其受壓跼部肌肉及腎髒進行病理切片檢查. 結果 所有大鼠解壓3 h後受壓肢體均明顯腫脹(P＜0.05).各組中K+、CK、Cr和BUN均隨壓迫麵積、時間及重量的增加和延長而明顯上升(P＜0.05).跼部受壓肌肉呈明顯水腫和壞死錶現;腎髒齣現腎小毬、腎小管充血,壓迫重量≥3 kg、壓迫時間≥6 h組可見腎小管壞死及管型. 結論壓迫時間、重量及麵積的增加和延長可增加擠壓傷跼部損傷和全身反應的嚴重程度.受壓麵積為雙側後肢、壓迫重量≥3 kg、壓迫時間≥6 h可較為穩定地造成大鼠典型的擠壓綜閤徵錶現,可初步作為建立擠壓綜閤徵大鼠模型的實驗條件.
목적 연구압박면적、압박시간화압박중량여국부손상급전신병리생리반응적상관성,초보모색가중복적제압종합정대서모형. 방법 청길급웅성SD대서72지,안수궤수자표법분위18개소조(매조4지대서).장압박면적(단、쌍측후지)、압박중량(2,3,4 kg)화압박시간(4,6,8 h)진행배렬조합후형성18충압박조건.소유대서재압박개시전화해압후3 h측량수압후지주경,동시취혈검측K+、CK、Cr화BUN,병재실험과정중관찰기홍단백뇨적발생솔;해압후24 h취기수압국부기육급신장진행병리절편검사. 결과 소유대서해압3 h후수압지체균명현종창(P＜0.05).각조중K+、CK、Cr화BUN균수압박면적、시간급중량적증가화연장이명현상승(P＜0.05).국부수압기육정명현수종화배사표현;신장출현신소구、신소관충혈,압박중량≥3 kg、압박시간≥6 h조가견신소관배사급관형. 결론압박시간、중량급면적적증가화연장가증가제압상국부손상화전신반응적엄중정도.수압면적위쌍측후지、압박중량≥3 kg、압박시간≥6 h가교위은정지조성대서전형적제압종합정표현,가초보작위건립제압종합정대서모형적실험조건.
Objective To investigate the relationship of compression area, time and weight as risky factors with local injury and systemic pathophysiological responses in rats so as to establish repeatable experimental model of crush syndrome. Methods A total of 144 male SD rats were divided into two groups, ie, mortality investigation group and biochemical indicator investigation group. Every group included the same 18 subgroups based on 18 kinds of combination with different levels of compression area (the right or both hind limbs), time (4, 6, 8 hours) and weight (2, 3, 4 kg). The circumference of the compressed hind limbs of all rats were measured and serum potassium (K+), serum creatine phosphokinase (CK), creatinine and carbamide were measured too before compression and three hours after decompression. Incidence of myoglobinuria of all rats was recorded. Muscles and kidneys were evaluated morphologically. Results The compressed hind limbs of all rats swelled significantly after three hours of reperfusion (P ＜ 0. 05). All serum K + , CK, CR and BUN were increased significantly with the increase and prolongation of the compression area, time and weight (P ＜ 0.05). Signs of direct cellular damage and ischemia-reperfusion injury were found in histology specimens of local compressed muscle.Hyperemia of glomeruli and renal tubule was found in the kidneys. Renal tubular necrosis and renal tubular cast were observed in group with compression weight ≥3 kg and compression duration ≥6 hours.Conclusions Increase and prolongation of the compression area, time and weight can aggravate the severity of crush injury. Compression area is more risky factor of severe crush injury. Both hind limbs ascompression area, compression weight ≥ 3 kg and compression duration ≥ 6 hours can be the effective experimental conditions for establishment of crush syndrome model in rats.
