中国临床实用医学
中國臨床實用醫學
중국림상실용의학
CHINA CLINICAL PRACTICAL MEDICINE
2010年
10期
6-8
,共3页
张翼飞%姜立新%郑海涛%郭吉田%胡金晨%吕忠船
張翼飛%薑立新%鄭海濤%郭吉田%鬍金晨%呂忠船
장익비%강립신%정해도%곽길전%호금신%려충선
结直肠癌%E-钙粘素%淋巴结转移%逆转录聚合酶链反应
結直腸癌%E-鈣粘素%淋巴結轉移%逆轉錄聚閤酶鏈反應
결직장암%E-개점소%림파결전이%역전록취합매련반응
colorectal carcinoma%E-Cadherin%Lymphatic metastasis%Rt-pcr
目的 检测结直肠癌组织中E-钙粘素的表达及其淋巴结微转移,探讨两者间的关系.方法 以CK20 mRNA为标记物,应用RT-PCR对60例结直肠癌根治性手术切除的淋巴结标本共782例进行检测;免疫组化法(SP法)检测结直肠癌组织、癌旁组织、淋巴结微转移及正常对照组中E-钙粘素的表达.通过二者的综合分析,试图发现抑癌基因E-钙粘素在结直肠癌淋巴结微转移的生物学行为中的作用.结果 CK20法检出45例(75.0%)患者有淋巴结转移,阳性淋巴结总数为436个(55.8%);常规病理学检出34例(56.7%)患者有淋巴结转移,检出阳性淋巴结数为356个(45.5%);两组比较,差异具有统计学意义(x2=16.72,P＜0.05).11例检出淋巴结微转移患者癌组织中6例(54.5%)E-Cadherin表达阴性,而15例未检出淋巴结微转移癌组织中仅有1例(6.67%)表达阴性,两者比较差异有统计学意义(x2=4.160,P=0.041).结论 E-Cadherin的表达减弱或消失参与了结直肠癌淋巴结微转移的发生.
目的 檢測結直腸癌組織中E-鈣粘素的錶達及其淋巴結微轉移,探討兩者間的關繫.方法 以CK20 mRNA為標記物,應用RT-PCR對60例結直腸癌根治性手術切除的淋巴結標本共782例進行檢測;免疫組化法(SP法)檢測結直腸癌組織、癌徬組織、淋巴結微轉移及正常對照組中E-鈣粘素的錶達.通過二者的綜閤分析,試圖髮現抑癌基因E-鈣粘素在結直腸癌淋巴結微轉移的生物學行為中的作用.結果 CK20法檢齣45例(75.0%)患者有淋巴結轉移,暘性淋巴結總數為436箇(55.8%);常規病理學檢齣34例(56.7%)患者有淋巴結轉移,檢齣暘性淋巴結數為356箇(45.5%);兩組比較,差異具有統計學意義(x2=16.72,P＜0.05).11例檢齣淋巴結微轉移患者癌組織中6例(54.5%)E-Cadherin錶達陰性,而15例未檢齣淋巴結微轉移癌組織中僅有1例(6.67%)錶達陰性,兩者比較差異有統計學意義(x2=4.160,P=0.041).結論 E-Cadherin的錶達減弱或消失參與瞭結直腸癌淋巴結微轉移的髮生.
목적 검측결직장암조직중E-개점소적표체급기림파결미전이,탐토량자간적관계.방법 이CK20 mRNA위표기물,응용RT-PCR대60례결직장암근치성수술절제적림파결표본공782례진행검측;면역조화법(SP법)검측결직장암조직、암방조직、림파결미전이급정상대조조중E-개점소적표체.통과이자적종합분석,시도발현억암기인E-개점소재결직장암림파결미전이적생물학행위중적작용.결과 CK20법검출45례(75.0%)환자유림파결전이,양성림파결총수위436개(55.8%);상규병이학검출34례(56.7%)환자유림파결전이,검출양성림파결수위356개(45.5%);량조비교,차이구유통계학의의(x2=16.72,P＜0.05).11례검출림파결미전이환자암조직중6례(54.5%)E-Cadherin표체음성,이15례미검출림파결미전이암조직중부유1례(6.67%)표체음성,량자비교차이유통계학의의(x2=4.160,P=0.041).결론 E-Cadherin적표체감약혹소실삼여료결직장암림파결미전이적발생.
Objective Purpose To detect the expression of E-cadherin in colorectal carcinoma and lymph node micrometastases,and investigate their relationship.Methods 82 cases of lymph node simple was dectected with RT-PCR method using CK20 mRNA acridinium ester.in screening colorectal carcinoma lymph node micrometastases.E-Cadherin expression analysed by immuno-histochemical staining(the SP method) in colorectal cancer tissue,tissue nearby cancer,the normal control tissue.Results In 60 cases of colorectal carcinoma,the expression of E-Cadherin was 71.67%.Number of positive nodes was 55.8%,the expression of E-cadherin was 56.7%(34 cases) and number of positive nodes was 45.5%(356 cases) through routine pathological examination.The low level expression of E-Cadherin was positively correlated with the Dukes staging,serosa infiltration,lymph node and liver metastasis of colorectal carcinoma(x2 = 16.72,P ＜ 0.05).The expression of E-Cadherin was negative in 6 of the 11 patients with lymph node micrometastases,and in 6.67% 1 of the 15 patients without lymph node micrometastases(x2 = 4.160,P = 0.041).Conclusion The negative expression of E-Cadherin may contribute to lymph node micrometastases in colorectal carcinoma.
