中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2011年
3期
219-222
,共4页
罗嘉%陶一明%罗来邦%杨连粤
囉嘉%陶一明%囉來邦%楊連粵
라가%도일명%라래방%양련월
肝细胞癌%SEMA3F%复发转移%预后
肝細胞癌%SEMA3F%複髮轉移%預後
간세포암%SEMA3F%복발전이%예후
Hepatocellular carcinoma%Semaphorin 3F%Recurrence/metastasis%Prognosis
目的 研究SEMA3F蛋白在肝细胞癌(hepatocelluar carcinoma,HCC)中的表达及其预后价值.方法 采用Western Blotting法检测32例HCC及相应癌旁肝组织标本中SEMA3F蛋白的表达.运用免疫组织化学法检测38例HCC以及其相应癌旁组织中的SEMA3F蛋白的表达和微血管密度(microvassel dainty,MVD)值,分析SEMA3F蛋白表达水平与MVD的相关性.结果 SEMA3F蛋白在32例HCC组织中的表达明显低于相应的PCLT(447.78±48.26比618.93±61.23,P＜0.05).SEMA3F在HCC组织中的表达水平与肿瘤的结节数目及是否具有包膜结构密切相关(P＜0.05).SEMA3F低表达组的复发转移率显著高于高表达组(P＜0.01)而其术后生存率则显著低于高表达组(P＜0.01).SEMA3F高表达组的MVD值明显低于低表达组(86.56±17.94比115.6±30.38,P＜0.01).结论 SEMA3F在HCC组织中表达下调与肝癌复发转移率高和预后差密切相关,提示其可能是一个潜在的HCC预后分子标志物.
目的 研究SEMA3F蛋白在肝細胞癌(hepatocelluar carcinoma,HCC)中的錶達及其預後價值.方法 採用Western Blotting法檢測32例HCC及相應癌徬肝組織標本中SEMA3F蛋白的錶達.運用免疫組織化學法檢測38例HCC以及其相應癌徬組織中的SEMA3F蛋白的錶達和微血管密度(microvassel dainty,MVD)值,分析SEMA3F蛋白錶達水平與MVD的相關性.結果 SEMA3F蛋白在32例HCC組織中的錶達明顯低于相應的PCLT(447.78±48.26比618.93±61.23,P＜0.05).SEMA3F在HCC組織中的錶達水平與腫瘤的結節數目及是否具有包膜結構密切相關(P＜0.05).SEMA3F低錶達組的複髮轉移率顯著高于高錶達組(P＜0.01)而其術後生存率則顯著低于高錶達組(P＜0.01).SEMA3F高錶達組的MVD值明顯低于低錶達組(86.56±17.94比115.6±30.38,P＜0.01).結論 SEMA3F在HCC組織中錶達下調與肝癌複髮轉移率高和預後差密切相關,提示其可能是一箇潛在的HCC預後分子標誌物.
목적 연구SEMA3F단백재간세포암(hepatocelluar carcinoma,HCC)중적표체급기예후개치.방법 채용Western Blotting법검측32례HCC급상응암방간조직표본중SEMA3F단백적표체.운용면역조직화학법검측38례HCC이급기상응암방조직중적SEMA3F단백적표체화미혈관밀도(microvassel dainty,MVD)치,분석SEMA3F단백표체수평여MVD적상관성.결과 SEMA3F단백재32례HCC조직중적표체명현저우상응적PCLT(447.78±48.26비618.93±61.23,P＜0.05).SEMA3F재HCC조직중적표체수평여종류적결절수목급시부구유포막결구밀절상관(P＜0.05).SEMA3F저표체조적복발전이솔현저고우고표체조(P＜0.01)이기술후생존솔칙현저저우고표체조(P＜0.01).SEMA3F고표체조적MVD치명현저우저표체조(86.56±17.94비115.6±30.38,P＜0.01).결론 SEMA3F재HCC조직중표체하조여간암복발전이솔고화예후차밀절상관,제시기가능시일개잠재적HCC예후분자표지물.
Objective To investigate the expression of Semaphorin 3F (SEMA3F) protein in hepatocellular carcinoma (HCC) and to demonstrate its relationship with clinicopathological features and prognosis of HCC. Methods Western Blotting was carried out in 32 hepatocellular carcinoma samples and matched perineoplastic tissues to detect the expression of SEMA3F protein. The relationship between SEMA3F protein expression and clinicopathological features as well as prognosis of HCC patients was analyzed. Immunohistochemistry was used to show the location of SEMA3F in HCC cells and its relationship with microvessel density (MVD). Results The expression of SEMA3F protein in HCC tissues was significantly higher than in the perineoplastic tissues (447.78± 48.26 vs 618.93 ±61.23, P＜0. 05) and it was correlated closely with tumor capsulation and tumor nodular number (P＜0.05). Based on the Western Blotting and clinical follow-up data, we found that the survival time of HCC patients with a higher SEMA3F expression level was longer than those with a lower level, and the recurrent/metastatic time of HCC patients was significantly different between these two groups (P＜0.01). Immunohistochemistry demonstrated that SEMA3F protein localized in the cytoplasm of HCC cells and its expression correlated with HCC MVD. MVD in the low-level group was higher than the high-level group (115.6±30.38 vs 86. 56±17.94, P＜0.01). Conclusions SEMA3F expression in HCC was significantly down-regulated and correlated closely with tumor-capsulation, nodular number, and MVD, implicating SEMA3F may play an important role in recurrence and metastasis of HCC. It can be regarded as a prognostic marker in HCC patients.
