中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2009年
8期
1055-1056
,共2页
陈小刚%张青汉%叶绪龙%王瑜%陈朝晖
陳小剛%張青漢%葉緒龍%王瑜%陳朝暉
진소강%장청한%협서룡%왕유%진조휘
肾肿瘤%Livin%基因表达
腎腫瘤%Livin%基因錶達
신종류%Livin%기인표체
Kidney neoplasma%Livin%Gene expression
目的 探讨凋亡抑制蛋白Livin的表达及与肾细胞癌发生、发展的关系.方法 应用免疫组织化学及逆转录-聚合酶链反应(RT-PCR)技术检测38例肾细胞癌和10例正常肾组织中Livin的表达.结果 免疫组织化学及RT-PCR法检测38例肾细胞癌中Livin的阳性率分别为57.89%(22/38)、60.53%(23/38);而10例正常肾组织中Livin均无表达,肾癌组和对照组差异有统计学意义(P<0.01).Livin表达率与肾细胞癌的病理类型、临床分期、病理分级、肿瘤大小无明显相关性(P>0.05).结论 Livin在肾癌的发生、发展中起重要作用,可能成为肾细胞癌的重要诊断指标和治疗靶点.
目的 探討凋亡抑製蛋白Livin的錶達及與腎細胞癌髮生、髮展的關繫.方法 應用免疫組織化學及逆轉錄-聚閤酶鏈反應(RT-PCR)技術檢測38例腎細胞癌和10例正常腎組織中Livin的錶達.結果 免疫組織化學及RT-PCR法檢測38例腎細胞癌中Livin的暘性率分彆為57.89%(22/38)、60.53%(23/38);而10例正常腎組織中Livin均無錶達,腎癌組和對照組差異有統計學意義(P<0.01).Livin錶達率與腎細胞癌的病理類型、臨床分期、病理分級、腫瘤大小無明顯相關性(P>0.05).結論 Livin在腎癌的髮生、髮展中起重要作用,可能成為腎細胞癌的重要診斷指標和治療靶點.
목적 탐토조망억제단백Livin적표체급여신세포암발생、발전적관계.방법 응용면역조직화학급역전록-취합매련반응(RT-PCR)기술검측38례신세포암화10례정상신조직중Livin적표체.결과 면역조직화학급RT-PCR법검측38례신세포암중Livin적양성솔분별위57.89%(22/38)、60.53%(23/38);이10례정상신조직중Livin균무표체,신암조화대조조차이유통계학의의(P<0.01).Livin표체솔여신세포암적병리류형、림상분기、병리분급、종류대소무명현상관성(P>0.05).결론 Livin재신암적발생、발전중기중요작용,가능성위신세포암적중요진단지표화치료파점.
Objective To study the expression of Livin,an apoptosis inhibitor,in tissues of renal cell carcinoma,and its significance.Methods Immunohistochemical and RT-PCR methods were used to detect the Livin expression in 38 cases of renal cell carcinoma, and 10 cases of normal kidney tissue.Resuits The positive rate of Livin expression in renal cell carcinoma was 57.89% (22/38) ,60.53% (23/ 38) respectively by immunohistocbemieal and RT-PCR methods, higher than in normal renal tissue (P < 0.01).There was no relationship between Livin expression and tumor size, histologic subtype, clinical stage and tumor grade of differentiation.Conclusion Livin had a relationship with biological behavior of renal cell carcinoma.Livin may play important roles in occurrence and progression of renal cell carcinoma.Livin can be important diagnostic/therapeutic targets in renal cell carcinoma.
