中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2012年
7期
450-454
,共5页
郭凌云%李俊霞%苗林%迟雁%王化虹%姬广聚%刘新光
郭凌雲%李俊霞%苗林%遲雁%王化虹%姬廣聚%劉新光
곽릉운%리준하%묘림%지안%왕화홍%희엄취%류신광
马来酸盐类%曲美布汀%应激%肌,平滑%结肠%钾通道,钙激活%兰尼碱受体钙释放通道
馬來痠鹽類%麯美佈汀%應激%肌,平滑%結腸%鉀通道,鈣激活%蘭尼堿受體鈣釋放通道
마래산염류%곡미포정%응격%기,평활%결장%갑통도,개격활%란니감수체개석방통도
Maleates%Trimebutine%Stress%Muscle,smooth%Colon%Potassium channels,Calcium-activated%Ryanodine receptor calcium release channel
目的 探讨马来酸曲美布汀(TM)对寒冷束缚应激(CRS)大鼠结肠平滑肌细胞大电导钙激活的钾通道(BKCa)及雷诺定受体(RyR) mRNA和蛋白表达的影响.方法 24只Wistar大鼠均分为CRS组、CRS+TM组和对照组,CRS组大鼠每日按6 ml/kg予0.9％ NaCl溶液灌胃,CRS+TM组大鼠每日按6 ml/kg予15 g/L TM灌胃,置铁丝笼中,在4℃环境限制活动2h,连续5d.对照组大鼠按6 ml/kg予0.9％NaCl溶液灌胃1次,不予应激.观察各组大鼠排便量和粪便性状改变,分离结肠平滑肌组织进行RT-PCR和Western印迹分析测定BKCa以及RyR2通道mRNA和蛋白的表达.结果 CRS组大鼠排便颗粒中位数为6,对照组为1,CRS+ TM组为5,肉眼观察CRS组及CRS+ TM组大鼠粪便外观较对照组稀,含水量多.与对照组比较,CRS组及CRS+ TM组大鼠结肠组织无明显病理学改变.对照组与CRS组大鼠结肠平滑肌细胞BKCa和RyR2 mRNA表达无明显差异,CRS+TM组BKCa mRNA表达较对照组上调1.45倍,CRS+ TM组RyR2 mRNA较对照组上调1.32倍.与对照组相比,CRS组大鼠结肠平滑肌细胞BKCa和RyR2蛋白表达无明显差异,CRS+TM组BKCa蛋白较对照组表达上调1.39倍,未见RyR2蛋白表达条带.结论 TM对CRS大鼠结肠平滑肌收缩的影响可能是通过大鼠结肠平滑肌细胞BKCa通道mRNA、蛋白表达上调和RyR mRNA表达上调而起作用.
目的 探討馬來痠麯美佈汀(TM)對寒冷束縳應激(CRS)大鼠結腸平滑肌細胞大電導鈣激活的鉀通道(BKCa)及雷諾定受體(RyR) mRNA和蛋白錶達的影響.方法 24隻Wistar大鼠均分為CRS組、CRS+TM組和對照組,CRS組大鼠每日按6 ml/kg予0.9％ NaCl溶液灌胃,CRS+TM組大鼠每日按6 ml/kg予15 g/L TM灌胃,置鐵絲籠中,在4℃環境限製活動2h,連續5d.對照組大鼠按6 ml/kg予0.9％NaCl溶液灌胃1次,不予應激.觀察各組大鼠排便量和糞便性狀改變,分離結腸平滑肌組織進行RT-PCR和Western印跡分析測定BKCa以及RyR2通道mRNA和蛋白的錶達.結果 CRS組大鼠排便顆粒中位數為6,對照組為1,CRS+ TM組為5,肉眼觀察CRS組及CRS+ TM組大鼠糞便外觀較對照組稀,含水量多.與對照組比較,CRS組及CRS+ TM組大鼠結腸組織無明顯病理學改變.對照組與CRS組大鼠結腸平滑肌細胞BKCa和RyR2 mRNA錶達無明顯差異,CRS+TM組BKCa mRNA錶達較對照組上調1.45倍,CRS+ TM組RyR2 mRNA較對照組上調1.32倍.與對照組相比,CRS組大鼠結腸平滑肌細胞BKCa和RyR2蛋白錶達無明顯差異,CRS+TM組BKCa蛋白較對照組錶達上調1.39倍,未見RyR2蛋白錶達條帶.結論 TM對CRS大鼠結腸平滑肌收縮的影響可能是通過大鼠結腸平滑肌細胞BKCa通道mRNA、蛋白錶達上調和RyR mRNA錶達上調而起作用.
목적 탐토마래산곡미포정(TM)대한랭속박응격(CRS)대서결장평활기세포대전도개격활적갑통도(BKCa)급뢰낙정수체(RyR) mRNA화단백표체적영향.방법 24지Wistar대서균분위CRS조、CRS+TM조화대조조,CRS조대서매일안6 ml/kg여0.9％ NaCl용액관위,CRS+TM조대서매일안6 ml/kg여15 g/L TM관위,치철사롱중,재4℃배경한제활동2h,련속5d.대조조대서안6 ml/kg여0.9％NaCl용액관위1차,불여응격.관찰각조대서배편량화분편성상개변,분리결장평활기조직진행RT-PCR화Western인적분석측정BKCa이급RyR2통도mRNA화단백적표체.결과 CRS조대서배편과립중위수위6,대조조위1,CRS+ TM조위5,육안관찰CRS조급CRS+ TM조대서분편외관교대조조희,함수량다.여대조조비교,CRS조급CRS+ TM조대서결장조직무명현병이학개변.대조조여CRS조대서결장평활기세포BKCa화RyR2 mRNA표체무명현차이,CRS+TM조BKCa mRNA표체교대조조상조1.45배,CRS+ TM조RyR2 mRNA교대조조상조1.32배.여대조조상비,CRS조대서결장평활기세포BKCa화RyR2단백표체무명현차이,CRS+TM조BKCa단백교대조조표체상조1.39배,미견RyR2단백표체조대.결론 TM대CRS대서결장평활기수축적영향가능시통과대서결장평활기세포BKCa통도mRNA、단백표체상조화RyR mRNA표체상조이기작용.
Objective To investigate the effects of trimebutine maleate (TM) on the expression of large conductance calcium-activated potassium channel (BKCa) and ryanodine receptors (RyR)channels at mRNA and protein level in colonic smooth muscle cell of cold restraint stress(CRS)induced rats.Methods A total of 24 Wistar rats were divided into CRS group,CRS with TM group and control group equally.The rats of CRS group were gavaged with 0.9％NaCl (6 ml/kg) daily; the rats of CRS with TM group were gavaged with 15 g/L TM (6 ml/kg) daily and activity was restricted in wire cage at 4 ℃ for two hours,continuously for five days.The rats of control group were gavaged with 0.9 ％ NaCl (6 ml/kg) once without CRS.The amount and characteristics of stool of rats in each group were observed.The colonic smooth muscle was isolated to detect the expression of BKCa and RyR at mRNA and protein level by reverse transcription-polymerase chain reaction (RT-PCR) and Western Blot.Results The median of rats defecation particles of CRS group was six,control group was one and CRS with TM group was five.Compared with control group,the defecation appearance of CRS group and CRS with TM group was looser and wetter observed by naked eyes.Compared with control group,there was no obvious pathological changes in CRS and CRS with TM group.There was no significant difference in the mRNA expression of BKCa and RyR channels between control group and CRS group.Compared with control group,the BKCa expression at mRNA level of CRS with TM group increased 1.45 fold.Compared with control group,the RyR2 expression at mRNA level of CRS with TM group increased 1.32 fold.Compared with control group,the BKCa expression at protein level of CRS with TM group increased 1.39 fold,and there was no RyR2 expression band at protein level.Conclusion TM might affect colonic smooth muscle contraction through the upregulation of BKCa expression at mRNA and protein level and RyR expression at mRNA level.
