CAJ | 학술논문

利用PCR-DGGE系统研究了剑南春不同窖龄（2－50年）窖泥的微生物群落结构、种群演替趋势及其与环境因子的相关性。结果表明，2年窖龄窖泥的细菌丰度和多样性指数与5年样品相似，而10年窖龄窖泥的细菌丰度和多样性指数则明显增加，之后的10年基本持平，但50年时又显著下降。相同窖龄的窖泥样品中，中层的物种丰度和多样性指数均高于上层和下层，特别是在2～5年样品中这一趋势更加明显。DGGE和序列分析显示，窖泥中优势种群均分布在厚壁菌门（Phylum Firmicutes）的杆菌纲（Class Bacill）和梭菌纲（Class Clostrida），其中耐酸乳杆菌（Lactobacillus acetotolerans）、芽孢菌（Bacillus fordii）、未培养的瘤胃菌（uncultured Ruminococcaceaebacterium）和梭菌（uncultured Clostridia bacterium）为窖泥样品中的主要优势种群。典型对应分析显示，窖泥微生物群落结构与环境因子具有显著的相关性，其中有效磷、氨氮、pH对微生物群落结构的影响较大，其次是腐殖质，而水分含量的影响较小。
이용PCR-DGGE계통연구료검남춘불동교령（2－50년）교니적미생물군락결구、충군연체추세급기여배경인자적상관성。결과표명，2년교령교니적세균봉도화다양성지수여5년양품상사，이10년교령교니적세균봉도화다양성지수칙명현증가，지후적10년기본지평，단50년시우현저하강。상동교령적교니양품중，중층적물충봉도화다양성지수균고우상층화하층，특별시재2～5년양품중저일추세경가명현。DGGE화서렬분석현시，교니중우세충군균분포재후벽균문（Phylum Firmicutes）적간균강（Class Bacill）화사균강（Class Clostrida），기중내산유간균（Lactobacillus acetotolerans）、아포균（Bacillus fordii）、미배양적류위균（uncultured Ruminococcaceaebacterium）화사균（uncultured Clostridia bacterium）위교니양품중적주요우세충군。전형대응분석현시，교니미생물군락결구여배경인자구유현저적상관성，기중유효린、안담、pH대미생물군락결구적영향교대，기차시부식질，이수분함량적영향교소。
Bacterial community succession in pit mud of different age （from 2 years to 50 years）in Jiannanchun Group and its correlations with environmental factors were systematically studied by PCR-DGGE methods. The results were as follows： bacterial abundance and bacterial diversi- ty index displayed high similarity between 2-year-old and 5-year-old pit mud samples, however, they increased obviously in 10-year-old pit mud samples and then kept stable in 20-year-old pit mud samples, and then dramatically declined in 50-year-old pit mud samples; for pit mud of the same age, bacterial abundance and bacterial diversity index in medium layer were higher than that in the upper or lower layer, especially for pit mud samples of 2 ~5 years; DGGE profile and sequences analysis indicated that the dominant bacterial populations in pit mud samples were mainly distributed in Class Bacill and Clostrida of Phylum Firmicutes. The predominant bacteria included Lactobacillus acetotoleraus, Bacillus fordii, uncultured Ruminocaccaceae bacterium, and uncultured Clostriclia bacterium; CCA results revealed that there was significant correlations between environmental factors and bacterial community in pit mud. Among all environmental factors, available phosphorous, ammonia nitrogen and pH had stronger influence on bacterial community structure in pit mud, then followed by humics and moisture content the least.