CAJ | 학술논문

采用流式细胞仪,3H-TdR掺入和酶联免疫打点(enzyme-linked immunospot,ELISPOT)方法,研究妊娠免疫学指标的改变.妊娠晚期大鼠脾脏单个核细胞表面分子主要组织相容性抗原Ⅱ(MHC Ⅱ)明显下调,外周血单个核细胞表达CDllc明显减少,共激活分子B7-1和B7-2未见改变;脾脏和外周血单个核细胞中Th2细胞因子IL-10、IL-4表达增多,TGFβ阳性细胞数也明显增加,而Thl细胞因子IFN7的产生未受抑制.此外,脾脏和外周血中单个核细胞的抗原特异性增殖未见改变,而腹腔淋巴结细胞的增殖明显升高.脾脏单个核细胞在妊娠晚期分泌较少的抗原特异性抗体.提示妊娠期性激素具有免疫调节作用,可能与怀孕时Thl细胞介导的自身免疫性疾病得到缓解有关.
채용류식세포의,3H-TdR참입화매련면역타점(enzyme-linked immunospot,ELISPOT)방법,연구임신면역학지표적개변.임신만기대서비장단개핵세포표면분자주요조직상용성항원Ⅱ(MHC Ⅱ)명현하조,외주혈단개핵세포표체CDllc명현감소,공격활분자B7-1화B7-2미견개변;비장화외주혈단개핵세포중Th2세포인자IL-10、IL-4표체증다,TGFβ양성세포수야명현증가,이Thl세포인자IFN7적산생미수억제.차외,비장화외주혈중단개핵세포적항원특이성증식미견개변,이복강림파결세포적증식명현승고.비장단개핵세포재임신만기분비교소적항원특이성항체.제시임신기성격소구유면역조절작용,가능여부잉시Thl세포개도적자신면역성질병득도완해유관.
The immunological parameters were analyzed during pregnancy of Lewis rats by the methods of flow cytometry, thymidine incorporation and enzyme-linked immunospot (ELISPOT). MHC Ⅱ of spleen mononuclear cells (MNCs) and CD1 lc of periphery blood IMNCs was apparently downregulated in late pregnancy, while the costimulatory molecules B7-1 and B7-2 showed no difference. Increased expression of Th2 cytokines (IL-10, IL-4) and TGFβ was detected in the spleen and peripheral blood MNCs in the third trimester by flow cytometry. No suppression of Thl cytokine represented by IFNγ was found. Furthermore, antigen specific proliferation of spleen and peripheral blood MNCs was unchanged, but higher proliferation of MNCs from mesenteric lymph nodes was shown in late pregnancy. There was an inhibition of antigen specific antibody production in pregnancy examined by ELISPOT. These data indicate the immunomodulatory effects of sex-hormones in pregnancy, which may be related to the remission of T cell-mediated autoimmune diseases during pregnancy.