CAJ | 학술논문

在SD大鼠上应用多管微电极方法,观察微电泳GABA及其受体的拮抗剂或激动剂对杏仁外侧核(LA)抑制皮层A Ⅰ神经元声反应效应的影响.结果显示,电泳GABA能抑制皮层A Ⅰ区神经元的电活动,电泳GABAA受体拮抗剂bicuculline(BIC)则能易化其反应;电刺激LA能抑制皮层A I区听神经元声反应,电泳GABA产生类似于刺激LA的抑制效应;LA对皮层A Ⅰ区神经元的抑制效应能被BIC所翻转,而不能被甘氨酸受体拮抗剂strychnine所翻转,电泳GABAB型受体激动剂bac1ofen对神经元声反应无影响.上述结果表明,GABA可能是介导LA抑制皮层A Ⅰ区神经元声反应的最终递质,并且是通过GABAA受体起作用的.
재SD대서상응용다관미전겁방법,관찰미전영GABA급기수체적길항제혹격동제대행인외측핵(LA)억제피층A Ⅰ신경원성반응효응적영향.결과현시,전영GABA능억제피층A Ⅰ구신경원적전활동,전영GABAA수체길항제bicuculline(BIC)칙능역화기반응;전자격LA능억제피층A I구은신경원성반응,전영GABA산생유사우자격LA적억제효응;LA대피층A Ⅰ구신경원적억제효응능피BIC소번전,이불능피감안산수체길항제strychnine소번전,전영GABAB형수체격동제bac1ofen대신경원성반응무영향.상술결과표명,GABA가능시개도LA억제피층A Ⅰ구신경원성반응적최종체질,병차시통과GABAA수체기작용적.
Experiments were performed on Sprague Dawley rats with multibarrel microelectrode technique. The effects of acoustic response of A Ⅰ cortex neurons produced by electrical stimulation of lateral amygdaloid nucleus (LA) and the influence of GABA were observed. Experimental results showed that iontophoretic administration of GABA caused a pronounced inhibition of the electrical activity of A- Ⅰ neurons. Blockade of GABAA with bicuculline (BIC) facilitated the acoustic response. The acoustic response of A- Ⅰ neurons was inhibited when the LA was stimulated. Iontophoretic application of GABA resulted in a similar inhibitory effect as that of LA stimulation. Blockade of GABAA with bicuculline reversed the inhibitory effect of LA stimulation on the acoustic response of A- Ⅰ neurons. In contrast, application of strychnine, a glycine receptor antagonist, could not reverse the inhibitory effect of LA. Baclofen, a GABAB agonist, did not affect the acoustic response of the auditory neurons. These results indicate that GABA is the ultimate transmitter which mediates the LA stimulation-induced inhibition of the acoustic response of A- Ⅰ neurons in rats, possibly via the GABAAreceptor.