CAJ | 학술논문

犬精液常温保存技术是实现犬人工授精的重要环节,对大力推广犬人工授精具有重要意义。本试验参考国内外相关报道,选择了4种常温保存稀释液配方进行对比试验,通过对精子有效存活时间、总存活时间、存活指数等项目测定,分析它们对公犬精液品质的影响。筛选出C号、D号稀释液配方,14～18℃恒温下精子O级和5级保存时间分别达105.O±17.0h和114.0±12.0h、总存活时间为132.O±14.0h和150.0±16.0h、生存指数为71.2±10.4h和74.6±14.0h,极显著优于对照组（P〈0.01）。结果表明,C号、D号稀释液均为适宜犬精液常温保存理想的稀释液配方。
견정액상온보존기술시실현견인공수정적중요배절,대대력추엄견인공수정구유중요의의。본시험삼고국내외상관보도,선택료4충상온보존희석액배방진행대비시험,통과대정자유효존활시간、총존활시간、존활지수등항목측정,분석타문대공견정액품질적영향。사선출C호、D호희석액배방,14～18℃항온하정자O급화5급보존시간분별체105.O±17.0h화114.0±12.0h、총존활시간위132.O±14.0h화150.0±16.0h、생존지수위71.2±10.4h화74.6±14.0h,겁현저우우대조조（P〈0.01）。결과표명,C호、D호희석액균위괄의견정액상온보존이상적희석액배방。
Canine semen of normal temperature preservation technology is the important link of dogs with artificial insemination, to vigorously promote the artificial insemination has important significance in dogs. This test refers to the domestic and foreign relevant reports, selected 4 kinds of room temperature preservation of dilution formula Comparison test, sperm survival time, based on effective overall survival, survival index project determination, analysis of their finale semen quality influence. Screening out C, D dilution formula, 14 - 18℃ constant temperature preservation of sperm 0.5 time respectively reached 105.0±17.0 h, and 114.0±12.0 h. total survival time for 132.0± 14.0 h, and 150.0± 16.0 h, live index for the 71.2±10.4 h, and 74.6±14.0 h, significantly better than the Control group （P〈 0.01） The results show that, C, D diluted solution for suitability of canine semen preserved at room temperature desired dilution formuia,