CAJ | 학술논문

目的:探讨大鼠细菌性肺炎模型应用ω-3多不饱和脂肪酸(PUFA)脂肪乳治疗后,对肺部的保护作用.方法:建立大鼠铜绿假单胞菌肺炎模型,随机分为鱼油脂肪乳组(n=8)、中/长链脂肪乳组(n=8)和等渗盐水组(n=8).24 h后,检测肺泡灌洗液TNF-α、IL-1β和IL-10水平.肺组织切片行H-E染色,病理评分.结果:鱼油脂肪乳组大鼠肺泡灌洗液中TNF-α水平与其他两组比较无显著性差异,但IL-1β和IL-10生成减少.肺组织病理评分无显著性差异.结论:ω-3 PUFA脂肪乳可下调部分炎民性介质的表达,但对细菌性肺炎24 h内进展无明显影响.
목적:탐토대서세균성폐염모형응용ω-3다불포화지방산(PUFA)지방유치료후,대폐부적보호작용.방법:건립대서동록가단포균폐염모형,수궤분위어유지방유조(n=8)、중/장련지방유조(n=8)화등삼염수조(n=8).24 h후,검측폐포관세액TNF-α、IL-1β화IL-10수평.폐조직절편행H-E염색,병리평분.결과:어유지방유조대서폐포관세액중TNF-α수평여기타량조비교무현저성차이,단IL-1β화IL-10생성감소.폐조직병리평분무현저성차이.결론:ω-3 PUFA지방유가하조부분염민성개질적표체,단대세균성폐염24 h내진전무명현영향.
Objective: To evaluate the protective effect and possible mechanism of ω-3 PUFA supplementation in the early stage of experimental acute bacterial pneumonia in mice.Methods: Mice of Pseudomonas aeruginosa (PA)-induced lung injury were randomly divided into three groups: fish oil supplementation treatment group (n = 8),soybean oil treatment group (n = 8) and normal sodium treatment group (n = 8).Evaluation criteria were inflammatory response 24 h after the injury assessed with TNF-α、IL-1β,IL-10 levels in bronchoalveolar lavage and lung injury evaluation by H-E staining.Results: Compared with the other two groups,the levels of IL-1β,IL-10 in fish oil supplementation treatment group were reduced.No difference was found in the TNF-α level and histological scoring among the groups.Conclusion: The ω-3 PUFA can decrease the elevated mediators of inflammation in bacterial pneumonia,but can't affect the pneumonia progress within 24 hours.