CAJ | 학술논문

目的:探讨脑钠素rs198388基因多态性与湖南汉族人群中原发性高血压易感性的关系.方法:选择高血压病人567例(高血压组),同期健康者555例(对照组).2组之间性别、年龄、抽烟和饮酒史差异无统计学意义.对2组进行血压测量,空腹12 h以上采血检测血糖、总胆固醇、甘油三酯、高密度脂蛋白胆固醇和低密度脂蛋白胆固醇等水平.应用聚合酶链反应-限制性片段长度多态性方法进行DNA多态性分析,琼脂糖凝胶电泳判断基因型.结果:检测到GG,GA,AA 3种基因型.在高血压病组与对照组之间基因型构成比差异有统计学意义(P＜0.05).高血压组GA和AA基因型及A等位基因频率(12.3%和6.9%)显著低于对照组(18.4%和9.7%,P=0.009和P=0.014).结论:脑钠素rs198388基因多态性可能与湖南地区汉族人群原发性高血压易感性有关,携带rs198388 GA和AA基因型及A等位基因者发生高血压的风险可能较小.
목적:탐토뇌납소rs198388기인다태성여호남한족인군중원발성고혈압역감성적관계.방법:선택고혈압병인567례(고혈압조),동기건강자555례(대조조).2조지간성별、년령、추연화음주사차이무통계학의의.대2조진행혈압측량,공복12 h이상채혈검측혈당、총담고순、감유삼지、고밀도지단백담고순화저밀도지단백담고순등수평.응용취합매련반응-한제성편단장도다태성방법진행DNA다태성분석,경지당응효전영판단기인형.결과:검측도GG,GA,AA 3충기인형.재고혈압병조여대조조지간기인형구성비차이유통계학의의(P＜0.05).고혈압조GA화AA기인형급A등위기인빈솔(12.3%화6.9%)현저저우대조조(18.4%화9.7%,P=0.009화P=0.014).결론:뇌납소rs198388기인다태성가능여호남지구한족인군원발성고혈압역감성유관,휴대rs198388 GA화AA기인형급A등위기인자발생고혈압적풍험가능교소.
Objective To investigate the relation between brain natriuretic peptide (BNP) rs198388 polymorphism and the susceptibility of essential hypertension in Han population of Hunan. Methods A total of 567 patients with hypertension (the hypertension group) and 555 healthy volunteers (the control group) were enrolled. Gender, age, smoking and drinking history of the 2 groups were not significantly different. Blood pressure was measured in the 2 groups. After fasting for 12 h or more, blood glucose, total cholesterol, triglycerides, high density lipoprotein cholesterol and low density lipoprotein cholesterol were measured. DNA polymorphism analysis was done by polymerase chain reaction-restriction fragment length polymorphism method, and genotype was determined by agarose gel electrophoresis. Results The GG, GA, and AA genotypeswere detected.The frequencies of GA and AA genotypes and A allele were significantly lower in the hypertension group (GA and AA:12.3%;A:6.9%) than those in the control group (GA and AA:18.4%; A:9.7%; P=0.009, and P=0.014, respectively). Conclusion BNP rs198388 polymorphism may be associated with essential hypertension in Han people in Hunan. Carrying rs198388 GA and AA genotypes and A allele may be the reason for low risk of hypertension.