CAJ | 학술논문

稻瘟菌(Magnaporthe oryzae)是一种半腐生病原菌,该病原菌可以侵染水稻,产生稻瘟病.对于水稻与稻瘟菌的互作机制,人们已经进行了大量的研究.然而,鉴于水稻基因组相对较大且稻瘟菌生理小种众多,致使研究进展缓慢.本研究通过将稻瘟菌生理小种Y34侵染拟南芥生态型Col-),发现Y34可以感染Col-0,从而建立Y34-拟南芥互作模型,用以探究Y34与抗白粉突变体edrl的关系.结果显示,edrl比Col-0对于稻瘟菌更敏感.之前有研究表明,降低SA含量的突变体(pad4,sid2,niml)均可抑制edrl相关表型.接种Y34于edrl与pad4、sid2、niml形成的双基因突变体发现,所有双突变体的感病性比edrl单突均明显降低.推测EDRI可能在拟南芥抗稻瘟菌方面起一定正调控作用,且其作用依赖于SA途径的相关基因.
도온균(Magnaporthe oryzae)시일충반부생병원균,해병원균가이침염수도,산생도온병.대우수도여도온균적호작궤제,인문이경진행료대량적연구.연이,감우수도기인조상대교대차도온균생리소충음다,치사연구진전완만.본연구통과장도온균생리소충Y34침염의남개생태형Col-),발현Y34가이감염Col-0,종이건립Y34-의남개호작모형,용이탐구Y34여항백분돌변체edrl적관계.결과현시,edrl비Col-0대우도온균경민감.지전유연구표명,강저SA함량적돌변체(pad4,sid2,niml)균가억제edrl상관표형.접충Y34우edrl여pad4、sid2、niml형성적쌍기인돌변체발현,소유쌍돌변체적감병성비edrl단돌균명현강저.추측EDRI가능재의남개항도온균방면기일정정조공작용,차기작용의뢰우SA도경적상관기인.
Magnaporthe oryzae (M. oryzae), a hemibiotrophic pathogenic fungus, can infect rice (Oryza sativa) and cause rice blast. The mechanisms involved in resistance of rice to blast have been studied extensively. However, it is difficult to investigate interactions between rice and M. oryzae because of the relatively large genome sequence of rice and the diversity of M. oryzae. The strain of M. oryzae, Y34, was found to be able to infect Arabidopsis thaliana Col-0 plants, so M. Oryzae (Y34)-Arabidopsis thaliana pathosystem was established to investigate the role of Arabidopsis thaliana ENHANCED DISEASE RESISTANCE 1 (AtEDR1), which exerts negative regulation on powder mildew resistance by salicylic acid (SA) pathway. The results indicated that edrl mutant was more susceptible to M. oryzae than wild type Col-0 plants. Previous double mutant analysis revealed that all edrl-associated disease resistance phenotypes were suppressed by mutants that block SA perception (niml) or reduced SA production (pad4 and sid2). These double mutants were inoculated with Y34 to examine if they would suppress the susceptibility of edrl to M. oryzae, and the results showed that compared with edrl, all double mutants exhibited resistance to M. oryzae. These data suggest AtEDR1 may play a positive role in the regulation of resistance to M. oryzae in Arabidopsis, which is required for SA pathway.