CAJ | 학술논문

目的探讨脑出血后异常积聚的铁离子在血肿周围组织病变中的作用及去铁胺的干预影响.方法将SD大鼠随机分为假手术组(n=15)、脑出血组(n=60)和去铁胺干预组(n=60),以尾状核注射自体血制作大鼠脑出血模型,观察各组大鼠不同时间点神经功能缺损评分、脑水含量(干湿重法)和血脑屏障通透性(伊文思蓝渗出法)、DNA碎裂(TUNEL染色)以及去铁胺干预的影响.结果脑出血组大鼠脑出血后1～7 d神经功能缺损评分显著高于假手术组(P＜0.01),血肿周围脑水含量显著增高(P＜0.05),血脑屏障通透性显著增加(P＜0.01),TUNEL阳性细胞数量显著增加(P＜0.05).去铁胺干预组各观察指标与脑出血组呈现相同变化趋势,但神经功能评分显著低于脑出血组(P＜0.05),血肿周围脑水含量显著低于脑出血组(P＜0.05),血脑屏障通透性显著低于脑出血组(P＜0.05),TUNEL阳性细胞数量显著少于脑出血组(P＜0.05).结论铁离子异常积聚参与了脑出血后血肿周围组织的病理损伤,去铁胺能减轻这一损伤.
목적 탐토뇌출혈후이상적취적철리자재혈종주위조직병변중적작용급거철알적간예영향.방법 장SD대서수궤분위가수술조(n=15)、뇌출혈조(n=60)화거철알간예조(n=60),이미상핵주사자체혈제작대서뇌출혈모형,관찰각조대서불동시간점신경공능결손평분、뇌수함량(간습중법)화혈뇌병장통투성(이문사람삼출법)、DNA쇄렬(TUNEL염색)이급거철알간예적영향.결과 뇌출혈조대서뇌출혈후1～7 d신경공능결손평분현저고우가수술조(P＜0.01),혈종주위뇌수함량현저증고(P＜0.05),혈뇌병장통투성현저증가(P＜0.01),TUNEL양성세포수량현저증가(P＜0.05).거철알간예조각관찰지표여뇌출혈조정현상동변화추세,단신경공능평분현저저우뇌출혈조(P＜0.05),혈종주위뇌수함량현저저우뇌출혈조(P＜0.05),혈뇌병장통투성현저저우뇌출혈조(P＜0.05),TUNEL양성세포수량현저소우뇌출혈조(P＜0.05).결론 철리자이상적취삼여료뇌출혈후혈종주위조직적병리손상,거철알능감경저일손상.
Objective To investigate the effect of the abnormally accumulated iron ions in the perihematoma tissue lesions and the impact of deferoxamine intervention. Methods A total of 135 SD rats were randomly assigned to sham-operation (n = 15), intracerebral hemorrhage (n =60) and deferoxamine (n =60) groups. A rat model of intracerebral hemorrhage was induced by the infusion of autologous blood. The neurological deficit score, brain water content (dry/wet weight method), blood-brain barrier permeability (Evans blue extravasation method), DNA fragmentation (TUNEL staining), and the impact of deferoxamine intervention were observed at different time points in all groups.Results One to seven days after intracerebral hemorrhage, the neurological deficit score, perihematomal water content, the blood-brain barrier permeability, and the numbers of TUNEL positive cells in the intracerebral hemorrhage group were significantly higher than those in the sham-operation group (P ＜0.01, P ＜0.05, P ＜0.01, and P ＜0.05). All the observation indexes in the deferoxamine and intracerebral hemorrhage groups showed the same trend, however, the neurological score, perihematomal water content, the blood brain barrier permeability, and the numbers of TUNEL positive cells were significantly lower than those in the intracerebral hemorrhage group (P ＜ 0.05, P ＜ 0.05, P ＜ 0.05, and P ＜ 0.05). Conclusions The abnormal accumulation of iron ions involves in the pathological injury of perihematoma tissue after intracerebral hemorrhage, and deferoxamine may reduce this injury.