CAJ | 학술논문

目的探讨肿瘤坏死因子受体1(TNFR1)在肝细胞肝癌(HCC)组织中的表达及其意义.方法应用免疫组织化学方法(SP法)检测76例HCC患者肿瘤组织和癌旁组织中TNFR1的蛋白表达,应用逆转录-聚合酶链反应(RT-PCR)检测其mRNA表达水平.结果 TNFR1在HCC中表达阳性率(39.5%),显著低于癌旁组织(89.5%,P＜0.05),并且随着肿瘤分级的增加,TNFR1在癌旁组织中表达量也递增;在基因水平TNFR1 mRNA的2-△△C1值在癌旁组织和癌组织中分别为0.0730±0.0200和0.0287±0.0079(P＜0.05),也同样得以验证.结论 TNFR1介导的凋亡在HCC的发展中占优势作用,TNFR1的表达增强与肝癌的发生及分化密切相关,在肝癌的发生发展中发挥重要作用.
목적 탐토종류배사인자수체1(TNFR1)재간세포간암(HCC)조직중적표체급기의의.방법 응용면역조직화학방법(SP법)검측76례HCC환자종류조직화암방조직중TNFR1적단백표체,응용역전록-취합매련반응(RT-PCR)검측기mRNA표체수평.결과 TNFR1재HCC중표체양성솔(39.5%),현저저우암방조직(89.5%,P＜0.05),병차수착종류분급적증가,TNFR1재암방조직중표체량야체증;재기인수평TNFR1 mRNA적2-△△C1치재암방조직화암조직중분별위0.0730±0.0200화0.0287±0.0079(P＜0.05),야동양득이험증.결론 TNFR1개도적조망재HCC적발전중점우세작용,TNFR1적표체증강여간암적발생급분화밀절상관,재간암적발생발전중발휘중요작용.
Objective To investigate the expression of tumor necrosis factor receptor 1 (TNFR1)in hepatocellular carcinoma (HCC) and its significance.Methods The expression of TNFR1 in 76 HCC tissues and adjacent carcinoma tissues was determined with immunohistochemical methods.Meanwhile,the expression of TNFR1 mRNA was analyzed by reverse transcription-polymerase chain reaction (RT-PCR).Results The expression of TNFR1 in HCC tissues in positive rate (39.5% ) was significantly lower than the adjacent tissues (89.5%,P ＜0.05 ),with the increase in tumor grade,the amount of TNFR1 in cancer adjacent tissues also increased;to further substantiate observations of TNFR1 expression,the level of TNFR1 mRNA values in the adjacent tissues and cancer tissues with a mean ± standard deviation were 0.0730±0.0200 and 0.0287 ±0.0079 (P＜0.05).Conclusion TNFR1 mediated apoptosis in HCC development in the dominant role,enhanced expression of TNFR1 closely related to the occurrence of liver cancer development and differentiation,and play an important role in liver cancer development.