CAJ | 학술논문

目的观察神经干细胞( NSCs)复合多肽自组装凝胶移植对大鼠脊髓损伤(SCI)后功能修复的影响.方法 36只SD大鼠造模后1周随机分为3组,分别为DMEM/F12对照组(n＝12)、NSCs移植组(n=12)和NSCs-凝胶移植组(n=12).通过不同时间点BBB评分、病理组织学、免疫荧光技术评价脊髓损伤的修复.结果移植后2周开始3组大鼠各时间点评分差异有统计学意义(P＜0.01),且组间差异均有统计学意义(P＜0.01)；移植后6周,病理切片示C组大量再生的神经纤维桥接脊髓断端,胶质瘢痕不明显；免疫荧光染色示C组5-溴脱氧尿嘧啶核苷(5-BrdU)/NF-200双标阳性细胞比例(24.83±1.47)％明显多于B组(6.83±1.47)％(P＜0.01),但B组BrdU/GFAP双标阳性细胞比例(42.17±2.71)％明显多于C组(34.33±4.63)％ (P＜0.01).结论自组装多肽凝胶能提高神经干细胞向神经元分化的比例,复合移植能更有效地促进脊髓功能恢复.
목적 관찰신경간세포( NSCs)복합다태자조장응효이식대대서척수손상(SCI)후공능수복적영향.방법 36지SD대서조모후1주수궤분위3조,분별위DMEM/F12대조조(n＝12)、NSCs이식조(n=12)화NSCs-응효이식조(n=12).통과불동시간점BBB평분、병리조직학、면역형광기술평개척수손상적수복.결과 이식후2주개시3조대서각시간점평분차이유통계학의의(P＜0.01),차조간차이균유통계학의의(P＜0.01)；이식후6주,병리절편시C조대량재생적신경섬유교접척수단단,효질반흔불명현；면역형광염색시C조5-추탈양뇨밀정핵감(5-BrdU)/NF-200쌍표양성세포비례(24.83±1.47)％명현다우B조(6.83±1.47)％(P＜0.01),단B조BrdU/GFAP쌍표양성세포비례(42.17±2.71)％명현다우C조(34.33±4.63)％ (P＜0.01).결론 자조장다태응효능제고신경간세포향신경원분화적비례,복합이식능경유효지촉진척수공능회복.
Objective To study the effects of co-transplatation of neural stem cells (NSCs) and the self-assembly peptide nanofiber hydrogels on the repair of transversal spinal cord injury in rats.Methods Thirty-six SD rats were randomly devided into groups A,B and C,which included 12 rats in each group after the animal models of spinal cord completely transsected had been established for one week.DMEM/F12 fluid,NSCs and composition of cells and hydrogels were transplanted into the caulal zone of the transected spinal cord in three groups repectively.The function repair was evaluated by Bundle branch block (BBB) score,pathology and imunnoflurorescence technique.Results The BBB scores in the three groups had significant difference at differernt time points (P ＜0.01 ),and the BBB scores were significantly different among the three groups ( P ＜ 0.01 ) after having been transplanted for two weeks.A great amount of regeneration host neuropils bridging at the two sides of the injured area and lesser glial scar were observed in group C after having been transplanted for six weeks.The percentage of BrdU/NF-200 double positive cells in group C ( 24.83 ± 1.47 ) ％ was higher than that in group B ( 6.83 ± 1.47 ) ％ ( P ＜0.01 ).However,the percentage of BrdU/GFAP double positive cells in group B (42.17 ± 2.71 ) ％ was higher than that in group C ( 34.33 ± 4.63 ) ％ ( P ＜ 0.01 ).Conclusion The self-assembly peptide nanofiber hydrogels could improve the differentiation of NSCs into neurons.Co-transplantation of NSCs with selfassembly of IKVAV peptide nanofiber hydrogels showed more effective results.