CAJ | 학술논문

目的探讨丁酸钠(Sodium Butyrate,NaB)抑制人肝癌细胞株SMMC-7721细胞生长、诱导其凋亡的分子机制.方法采用噻唑蓝(MTT)比色法、倒置显微镜观察药物对细胞生长的影响并绘制细胞增殖抑制曲线;透射电镜观察细胞凋亡的形态变化;流式细胞术分析细胞周期;半定量RT-PCR方法检测细胞p21WAF1基因mRNA的表达水平;Western blot检测细胞p21WAF1蛋白的表达变化.结果 NaB对人肝癌细胞生长的抑制呈剂量依赖和时间依赖关系.将细胞周期阻滞于G0/G1期,NaB上调p21WAF1 mRNA及蛋白的表达.结论 NaB具有抑制人肝癌SMMC-7721细胞增殖、诱导其凋亡的作用,这种作用可能是通过上调p21WAF1 mRNA及蛋白完成的.
목적 탐토정산납(Sodium Butyrate,NaB)억제인간암세포주SMMC-7721세포생장、유도기조망적분자궤제.방법 채용새서람(MTT)비색법、도치현미경관찰약물대세포생장적영향병회제세포증식억제곡선;투사전경관찰세포조망적형태변화;류식세포술분석세포주기;반정량RT-PCR방법검측세포p21WAF1기인mRNA적표체수평;Western blot검측세포p21WAF1단백적표체변화.결과 NaB대인간암세포생장적억제정제량의뢰화시간의뢰관계.장세포주기조체우G0/G1기,NaB상조p21WAF1 mRNA급단백적표체.결론 NaB구유억제인간암SMMC-7721세포증식、유도기조망적작용,저충작용가능시통과상조p21WAF1 mRNA급단백완성적.
Objective To explore the molecular mechanisms of sodium butyrate(NaB)inducing apoptosis of human hepatoma cell line 7721.Methods The hepatoma cells were treated with various concentrations of NaB in different durations in vitro.Proliferation suppression was observed bv MTT method and invert microscope,and morphological changes of apoptosis were observed by transmission electron microseopy(TEM).Flow cytometry(FCM)was used to investigate the apoptosis rate and the cell cycle.The expression of p21 mRNA and protein was detected by semi-quantitative RT-PCR and Western-blot respectively.Results The NaB inhibited the growth of bepatoma cells in a dose-dependent and time-dependent manner.Invert microscopy and TEM showed that the cells treated wlth NaB exhibited characteristics of apoptosis.NaB blocked cells mainly in the G0/G1 phase,but stimulated p21 expression both at the mRNA and protein levels.Condusion NaB effect on proliferation inhibition and apoptosis induction may be linked to its ability to up-regulate of D21 gene.