CAJ | 학술논문

目的观察乙型肝炎病毒(HBV)相关肝细胞癌的mRNA表达谱的变化,并进行生物信息学分析.方法用长链RNA芯片检测肝癌组织和正常肝组织mRNA的表达谱,并进行GO分析将差异表达的基因进行功能和细胞定位.结果　肝癌mRNA表达量与正常对照组比较,2倍以上变化,差异有统计学意义(P＜0.05)的mRNA认为是差异表达的mRNA,共991条,占所有基因总数的5.7%.其中2倍以上升高的共733条;2倍以上降低的共258条;5倍以上升高的共94条;5倍以上降低的共39条;10倍以上升高的共18条;10倍以上降低的共11条.GO分析表明差异性表达的mRNA参与了重要生物学调节功能,其中一些基因可能是HBV相关肝癌所特有的.结论　HBV相关肝癌的mRNA表达谱发生显著变化,差异基因参与了细胞的重要生物调节过程,可能参与肝癌的重要调节过程;HBV相关肝癌的表达谱与其他病因相关的肝癌有共性,也有一定的特性.
목적 관찰을형간염병독(HBV)상관간세포암적mRNA표체보적변화,병진행생물신식학분석.방법 용장련RNA심편검측간암조직화정상간조직mRNA적표체보,병진행GO분석장차이표체적기인진행공능화세포정위.결과　간암mRNA표체량여정상대조조비교,2배이상변화,차이유통계학의의(P＜0.05)적mRNA인위시차이표체적mRNA,공991조,점소유기인총수적5.7%.기중2배이상승고적공733조;2배이상강저적공258조;5배이상승고적공94조;5배이상강저적공39조;10배이상승고적공18조;10배이상강저적공11조.GO분석표명차이성표체적mRNA삼여료중요생물학조절공능,기중일사기인가능시HBV상관간암소특유적.결론　HBV상관간암적mRNA표체보발생현저변화,차이기인삼여료세포적중요생물조절과정,가능삼여간암적중요조절과정;HBV상관간암적표체보여기타병인상관적간암유공성,야유일정적특성.
Objective To explore mRNA expression profile variation of hepatitis B virus ( HBV)related hepatocellular carcinoma and analyze bioinformazics. Methods Long-chain RNA chip was used to inspect mRNA expression profile in HBV-related hepatocellular carcinoma and normal hepatic tissue and make GO analysis for function and cellular localization of genes with differential expression. Results By comparing mRNA expression level of liver cancer with normal control group, 991 mRNA which had more than 2 times variation and significant difference (P＜0.05 ) by statistical analysis were regarded as differential expression mRNA, accounting for 5.7% of all genes. 733 mRNA were increased by more than 2times and 258 decreased by more than 2 times; 94 increased by more than 5 times, 39 decreased by more than 5 times; 18 increased by more than 10 times, 11 decreased by more than 10 times. GO analysis revealed that differential expression mRNA involved in significant biological regulatory function, and some genes may be particular to HBV-related liver cancer. Conclusion The mRNA expression profile in HBVrelated liver cancer changes significantly, and the different genes participate in significant biological regulation process of cells and maybe participate in significant regulation process of liver cancer; HBV-related liver cancer' s expression profile has certain characteristics and something in common with liver cancers relating to other pathogenesis.