CAJ | 학술논문

目的探讨不同局部温热条件对人乳头瘤病毒(HPV)感染皮损朗格汉斯细胞(LCs)CD1a/CD83表达变化的影响,为病毒疣的治疗提供理论依据.方法采用共聚焦显微镜检测正常皮肤组织和尖锐湿疣皮损内LC CD1a/CD83的表达变化;利用37℃,42℃,45℃不同温热条件处理正常皮肤组织和尖锐湿疣皮损,采用流式分析技术检测游走至培养液中CD1a~+/CD83~+LC的数量变化.结果正常皮肤组织和尖锐湿疣皮损内只见CD1a~+LC,无CD83~+LC;正常皮肤组织和尖锐湿疣组织游走细胞中CD1a~+/CD83~+LC的百分比均随局部温度升高而逐渐增多.温热对尖锐湿疣组织游走LC中CD1a'CD83表达影响显著高于正常皮肤组织.结论局部温热可能通过促进LC的迁移和成熟,在局部细胞免疫应答中发挥作用.
목적 탐토불동국부온열조건대인유두류병독(HPV)감염피손랑격한사세포(LCs)CD1a/CD83표체변화적영향,위병독우적치료제공이론의거.방법 채용공취초현미경검측정상피부조직화첨예습우피손내LC CD1a/CD83적표체변화;이용37℃,42℃,45℃불동온열조건처리정상피부조직화첨예습우피손,채용류식분석기술검측유주지배양액중CD1a~+/CD83~+LC적수량변화.결과 정상피부조직화첨예습우피손내지견CD1a~+LC,무CD83~+LC;정상피부조직화첨예습우조직유주세포중CD1a~+/CD83~+LC적백분비균수국부온도승고이축점증다.온열대첨예습우조직유주LC중CD1a'CD83표체영향현저고우정상피부조직.결론 국부온열가능통과촉진LC적천이화성숙,재국부세포면역응답중발휘작용.
Objective To investigate the possible mechanism of local hyperthermia in the treatment of warts through detecting the differences in CD1a/CD83 of Langerhans cells(LCs) in émigrés from HPV-infected skin,as compared to normal skin.Methods Confocal microscopy were performed on Condyloma Aecuminatum (CA) and normal skin;Freshly taken biopsies of CA and normal skin were subjected to surface heating at 37℃,42T℃ and 45℃ respectively,for 30mins.Flow cytometry was used to determine the CD1a/CD83 changes of LCs in émigrés from CA and normal skin.Results By confocal microscopic observation,there were practically no CD1a~+ LCs that expressed CD83 in the epidermis of both normal skin and CA.The proportions of CD1a~+/CD83~+ LCs were significantly increased with increased temperatures in émigrés from both normal skin and CA.At each given temperature,the numbers of LCs in émigrés from CA were greater than those from normal skin.Conclusion Local hyperthermia can promote migration and maturation of LCs in HPV-infected skin and accordingly stimulate the immune system to treat warts.