中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2010年
1期
13-16
,共4页
股动脉%局部血流量%生理性缺血训练
股動脈%跼部血流量%生理性缺血訓練
고동맥%국부혈류량%생이성결혈훈련
Femoral artery%Local blood flow%Ischemic training
目的 观察不同等长收缩负荷对股动脉同有侧支循环开放的影响,为研究生理性缺血训练对侧支循环生成的影响奠定基础.方法 选择健康成年家兔24只,体重(2.0±0.5)kg,于左下肢坐骨神经植入电极,电刺激产生等长收缩造成缺血,预先设定不同收缩强度和时间.实验终止时取坐骨神经支配区-腓肠肌组织,微球技术定量检测股动脉固有侧支血流(FCBF).结果 ①采用40%最大等长收缩强度(IEmax)时缺血区局部血流降低程度最大(P<0.01);采用60%IEmax和80%IEmax的局部血流下降率相比,差异无统计学意义.②采用40%IEmax刺激0～4 min,FCBF呈增加趋势,第4分钟时达峰值,较刺激即刻、刺激后第2,3分钟明显增加(P<0.01),至第5分钟不再进一步增加.③以40%IEmax刺激4 min后再灌注0～2 min,FCBF呈增加趋势,再灌注第2分钟FCBF较再灌注即刻明显增加(P<0.01),第5分钟FCBF回落至基础水平,至第8,10分钟无显著变化.结论 促使家兔股动脉FCBF最大开放的最小强度为40%IEmax,最短刺激时间为4 min,FCBF开放持续时间不超过5 min.
目的 觀察不同等長收縮負荷對股動脈同有側支循環開放的影響,為研究生理性缺血訓練對側支循環生成的影響奠定基礎.方法 選擇健康成年傢兔24隻,體重(2.0±0.5)kg,于左下肢坐骨神經植入電極,電刺激產生等長收縮造成缺血,預先設定不同收縮彊度和時間.實驗終止時取坐骨神經支配區-腓腸肌組織,微毬技術定量檢測股動脈固有側支血流(FCBF).結果 ①採用40%最大等長收縮彊度(IEmax)時缺血區跼部血流降低程度最大(P<0.01);採用60%IEmax和80%IEmax的跼部血流下降率相比,差異無統計學意義.②採用40%IEmax刺激0～4 min,FCBF呈增加趨勢,第4分鐘時達峰值,較刺激即刻、刺激後第2,3分鐘明顯增加(P<0.01),至第5分鐘不再進一步增加.③以40%IEmax刺激4 min後再灌註0～2 min,FCBF呈增加趨勢,再灌註第2分鐘FCBF較再灌註即刻明顯增加(P<0.01),第5分鐘FCBF迴落至基礎水平,至第8,10分鐘無顯著變化.結論 促使傢兔股動脈FCBF最大開放的最小彊度為40%IEmax,最短刺激時間為4 min,FCBF開放持續時間不超過5 min.
목적 관찰불동등장수축부하대고동맥동유측지순배개방적영향,위연구생이성결혈훈련대측지순배생성적영향전정기출.방법 선택건강성년가토24지,체중(2.0±0.5)kg,우좌하지좌골신경식입전겁,전자격산생등장수축조성결혈,예선설정불동수축강도화시간.실험종지시취좌골신경지배구-비장기조직,미구기술정량검측고동맥고유측지혈류(FCBF).결과 ①채용40%최대등장수축강도(IEmax)시결혈구국부혈류강저정도최대(P<0.01);채용60%IEmax화80%IEmax적국부혈류하강솔상비,차이무통계학의의.②채용40%IEmax자격0～4 min,FCBF정증가추세,제4분종시체봉치,교자격즉각、자격후제2,3분종명현증가(P<0.01),지제5분종불재진일보증가.③이40%IEmax자격4 min후재관주0～2 min,FCBF정증가추세,재관주제2분종FCBF교재관주즉각명현증가(P<0.01),제5분종FCBF회락지기출수평,지제8,10분종무현저변화.결론 촉사가토고동맥FCBF최대개방적최소강도위40%IEmax,최단자격시간위4 min,FCBF개방지속시간불초과5 min.
Objective To explore the effect of isometric contraction-induced ischemic burden for evoking maximal intrinsic femoral artery collateral circulation. Methods Twenty-four adult New Zealand rabbits weighing (2.0±0.5) kg were subjected to a model of isometric contraction-induced ischemia. An electrode was implanted in the sciatic nerve of the left hind limb for evoking isometric contraction of the gastrocnemius, which blocked local blood flow and induced local ischemia. Femoral artery collateral blood flow (FCBF) was measured using a micro-sphere technique. Results Local blood flow was the lowest at 40% of the maximal isometric contraction (IEmax), and reached plateau at 60% of IEmax. FCBF increased gradually and reached a plateau at 40% of IEmax in the 4th min. After 4 minutes at 40% of IEmax, FCBF had increased to higher than pre-stimulus, peaking in the 2nd minute, and then decreasing gradually to baseline at the 5th minute after reperfusion. It ceased to increase when reperfusion was prolonged to 8 or 10 min. Conclusions The minimal ischemic burden for full obstruction of the femoral artery is 40% of IEmax, and the shortest stimulus duration is 4 min. The maximal FCBF opening duration is no more than 5 min.
