CAJ | 학술논문

构建了一种基于ZnO/Nafion复合膜固定双酶的葡萄糖传感器。这种新复合膜的优点是利用 ZnO 的比表面积大、吸附能力强、具有生物兼容性等性能来固定 HRP 和 GOD 酶，同时利用 Nafion 的成膜、抗干扰能力，制成了响应快速、灵敏度高的葡萄糖传感器。该传感器在最优化实验条件下响应时间小于5 s，以对苯二酚作为电子媒介检测葡萄糖的检测下限为5.0 mmol/L，线性范围为2.5×10-5 ~ 3×10-3 mol/L。
구건료일충기우ZnO/Nafion복합막고정쌍매적포도당전감기。저충신복합막적우점시이용 ZnO 적비표면적대、흡부능력강、구유생물겸용성등성능래고정 HRP 화 GOD 매，동시이용 Nafion 적성막、항간우능력，제성료향응쾌속、령민도고적포도당전감기。해전감기재최우화실험조건하향응시간소우5 s，이대분이분작위전자매개검측포도당적검측하한위5.0 mmol/L，선성범위위2.5×10-5 ~ 3×10-3 mol/L。
ZnO Nanorods synthesized by solvothermal route was dispersed in Nafion of sodium hexametaphosphate（（NaPO3）6） to form a stable aqueous dispersion for the fabrication of glucose biosensor.The parameters affecting the fabrication and experimental conditions of biosensors were optimized.The biosensor showed a fast response of less than 5 s and a detection limit of 5.0 mmol/L glucose by using hydroquinone as the mediator.Linear calibration for glucose was obtained in the rage 2.5×10-5 to 3×10-3 mol/L under the optimized conditions.