解放军医学杂志
解放軍醫學雜誌
해방군의학잡지
MEDICAL JOURNAL OF CHINESE PEOPLE'S LIBERATION ARMY
2001年
4期
244-245
,共2页
史培良%胡平%顾晓明%高峰%杨维东%毛天球
史培良%鬍平%顧曉明%高峰%楊維東%毛天毬
사배량%호평%고효명%고봉%양유동%모천구
聚羟基丁酸酯%骨髓基质细胞%组织工程
聚羥基丁痠酯%骨髓基質細胞%組織工程
취간기정산지%골수기질세포%조직공정
取4周龄新西兰兔骨髓,分离骨髓基质细胞,体外培养,经诱导分化为成骨细胞,接种于聚羟基丁酸酯(PHB)泡沫材料中,并植入裸鼠背部皮下,对照组单纯植入PHB。植入4、8周后行X线摄片和组织学观察,观察新骨形成情况。结果显示,PHB与骨髓基质成骨细胞植入裸鼠皮下4周后,局部形成骨组织,可见大量成骨细胞,某些部位可见红色的骨基质形成;8周形成大量新生骨组织,可见板层骨。对照组未见骨形成。
取4週齡新西蘭兔骨髓,分離骨髓基質細胞,體外培養,經誘導分化為成骨細胞,接種于聚羥基丁痠酯(PHB)泡沫材料中,併植入裸鼠揹部皮下,對照組單純植入PHB。植入4、8週後行X線攝片和組織學觀察,觀察新骨形成情況。結果顯示,PHB與骨髓基質成骨細胞植入裸鼠皮下4週後,跼部形成骨組織,可見大量成骨細胞,某些部位可見紅色的骨基質形成;8週形成大量新生骨組織,可見闆層骨。對照組未見骨形成。
취4주령신서란토골수,분리골수기질세포,체외배양,경유도분화위성골세포,접충우취간기정산지(PHB)포말재료중,병식입라서배부피하,대조조단순식입PHB。식입4、8주후행X선섭편화조직학관찰,관찰신골형성정황。결과현시,PHB여골수기질성골세포식입라서피하4주후,국부형성골조직,가견대량성골세포,모사부위가견홍색적골기질형성;8주형성대량신생골조직,가견판층골。대조조미견골형성。
To investigate the feasibility of using poly-beta-hydroxybutyrate as scaffolds for bone tissue engineering. Marrow stromal cells were harvested from New Zealand rabbit′s iliac bone. After being cultured and multiplied in vitro,with the use of dexamethasone to promote the osteoblastic phenotype of the cells, the cells were seeded into poly-beta-hydroxybutyrate. The cells/ poly-beta-hydroxybutyrate construction was implanted subcutaneously in nude mice. In control animals, poly-beta-hydroxybutyrate alone was implanted. Osteogenesis was assessed by histological and roentgenographic analysis. Four weeks after implantation, osteoid tissue had been observed in the specimens of the composite; 8 weeks after implantation, large amount of new bone had been found, and lamellar bone had been observed also. In the control, only fibrous tissue had been found. It suggested that poly-beta-hydroxybutyrate can be used as a scaffold material for bone tissue engineering.