CAJ | 학술논문

目的运用随机共振法对湮没在噪声中的弱色谱峰进行定量分析.方法基于随机共振理论,通过优化系统参数和Runge-Kutta方法来提高信噪比的方式建立了能提高色谱检测限的随机共振算法,并应用于HPLC/UV法定量检测人血浆中的非那吡啶浓度.同时将该方法与HPLC/MS法进行了比较.结果实验数据表明非那吡啶的浓度与响应值之间的线性关系良好.通过两种方法分别测得的受试者的血药浓度数据说明了这两种方法差异无显著性意义.结论该方法是可行的.
목적운용수궤공진법대인몰재조성중적약색보봉진행정량분석.방법기우수궤공진이론,통과우화계통삼수화Runge-Kutta방법래제고신조비적방식건립료능제고색보검측한적수궤공진산법,병응용우HPLC/UV법정량검측인혈장중적비나필정농도.동시장해방법여HPLC/MS법진행료비교.결과실험수거표명비나필정적농도여향응치지간적선성관계량호.통과량충방법분별측득적수시자적혈약농도수거설명료저량충방법차이무현저성의의.결론해방법시가행적.
Aim To apply stochastic resonance algorithm (SRA) to quantitative analysis of weak chromatographic signal, which was embedded in the noise. Methods Based on the theory of stochastic resonance (SR), a simple and effective SRA has been established to improve analytical detection limits of chromatographic analysis, which apply to enhance the signal to noise ratio by the optimization of the parameters and Runge-Kutta method, was established. The method was used to quantitative analysis of phenazopyridine in human plasma by HPLC/UV. Meanwhile this method is compared with HPLC/MS.Results By experimental chromatographic data sets, an excellent quantitative relationship between concentrations of phenazopyridine and their responses had been obtained. The concentration of phenazopyridine in plasma determined by HPLC/UV with SRA and HPLC/MS showed that there was no significant difference (P ＞ 0.05) between the two methods. Conclusion The new method was feasible.