CAJ | 학술논문

背景:莨菪碱类药物改善微循环和作为中药麻醉剂已在国内临床上广泛应用,但莨菪类生物碱抗吗啡成瘾作用的动物实验缺乏深入报道.目的:观察莨菪类生物碱与吗啡合用对抗吗啡致小鼠依赖性的作用,以期为开发防治阿片类物质成瘾的莨菪类药物提供实验学依据.设计:以实验动物为研究对象的随机对照实验.单位:一所大学医学院的生理学实验室.材料:本实验于2004-06/2004-08在河北工程学院医学部生理学实验室完成.选择健康雄性昆明小鼠50只,2月龄,体质量(20±2)g,由河北医科大学实验动物中心提供,清洁级.方法:依据吗啡成瘾动物的依赖性评价指标,选择痛阈和纳络酮跳台反应作为观察项目.将50只小鼠随机分为5组,即对照组、吗啡组、东莨菪碱组、山莨菪碱组和阿托品组,每组10只.分别于第1天至第7天每天用热板法观察腹腔注射生理盐水、吗啡及莨菪类生物碱合并吗啡后1 h的痛阈.并于末次(第7天)给药后6 h,腹腔注射纳络酮5 mg/kg催促,观察30 min内小鼠跳跃次数.主要观察指标:①吗啡成瘾小鼠的痛阈;②吗啡成瘾小鼠的跳跃次数和跳跃动物数(率).结果:与对照组比较,吗啡组小鼠的痛阈明显下降(P＜0.05或P＜0.01),跳跃次数和跳跃动物率明显增加.东莨菪碱与吗啡合用7 d后,明显提高吗啡依赖小鼠的痛阈(P＜0.01),并减少跳跃次数和跳跃动物率(P＜0.05);阿托品和山莨菪碱与吗啡合用在提高吗啡依赖小鼠痛阈方面作用较弱,但减少跳跃次数和跳跃动物率的作用较强(P＜0.01).结论:莨菪类生物碱均具有不同程度对抗吗啡依赖性的作用,为吗啡依赖患者的戒断作用提供重要的实验学依据.
배경:랑탕감류약물개선미순배화작위중약마취제이재국내림상상엄범응용,단랑탕류생물감항마배성은작용적동물실험결핍심입보도.목적:관찰랑탕류생물감여마배합용대항마배치소서의뢰성적작용,이기위개발방치아편류물질성은적랑탕류약물제공실험학의거.설계:이실험동물위연구대상적수궤대조실험.단위:일소대학의학원적생이학실험실.재료:본실험우2004-06/2004-08재하북공정학원의학부생이학실험실완성.선택건강웅성곤명소서50지,2월령,체질량(20±2)g,유하북의과대학실험동물중심제공,청길급.방법:의거마배성은동물적의뢰성평개지표,선택통역화납락동도태반응작위관찰항목.장50지소서수궤분위5조,즉대조조、마배조、동랑탕감조、산랑탕감조화아탁품조,매조10지.분별우제1천지제7천매천용열판법관찰복강주사생리염수、마배급랑탕류생물감합병마배후1 h적통역.병우말차(제7천)급약후6 h,복강주사납락동5 mg/kg최촉,관찰30 min내소서도약차수.주요관찰지표:①마배성은소서적통역;②마배성은소서적도약차수화도약동물수(솔).결과:여대조조비교,마배조소서적통역명현하강(P＜0.05혹P＜0.01),도약차수화도약동물솔명현증가.동랑탕감여마배합용7 d후,명현제고마배의뢰소서적통역(P＜0.01),병감소도약차수화도약동물솔(P＜0.05);아탁품화산랑탕감여마배합용재제고마배의뢰소서통역방면작용교약,단감소도약차수화도약동물솔적작용교강(P＜0.01).결론:랑탕류생물감균구유불동정도대항마배의뢰성적작용,위마배의뢰환자적계단작용제공중요적실험학의거.
BACKGROUND: Belladonna drugs have been widely used in clinic in our country to improve microcirculation, or as a herbal anesthetic drug. However,there are few reports regarding the animal experiments on belladonna alkaloid against morphine addiction further OBJECTIVE: To observe the effects of belladonna alkaloid combined with morphine on morphine(Mor)-addicted mice so as to provide an experimental basis for development of belladonna to morphine addiction.DESIGN: A completely randomized-controlled study based on the experimental animals.SETTING: Laboratory of physiology of a medical college.MATERIALS: The study was performed at the Laboratory of Physiology of Medical Department of Hebei Engineering College from June 2004 to August 2004. Fifty 2-month old male healthy Kunming mice of clean grade with a body mass of(20±2) g were obtained from Experimental Animal Centre of Hebei Medical University.METHODS: According to evaluation index of dependence in Morphine-addicted animals, we chose pain threshold and naloxone-urged jumping response as items to observe. Fifty mice were randomly divided into 5 groups with 10 mice each, which were the control group (saline), the morphine group, the scopolamine(Sco)group, the anisodamine(Ani), atropine(Atr)group. The corresponding drugs or saline was administered by intraperitoneal (I. P.) injection once a day for 7 days. The pain threshold at 1 hour after I. P. Injection of drugs was observed from day 1 to day 7 by hot-plate method. Mice were given I.p. Injection of naloxone (Nal, 5 mg/kg ) 6hours after the last injection. The jumping times within 30 minutes were observed to evaluate the ,formation of the Morphine addiction.Nal-urged mice.RESULTS: The pain threshold of the mice in Morphine group was decreased significantly, and the jumping times and jumping rate were obviously increased compared with that of the control group( P ＜ 0.05 or P ＜ 0.01).The co-administration of Sco-Mor mixtures for 7 days significantly increased the pain threshold of Mor-dependent mice( P ＜ 0.01) and markedly decreased the jumping times and the jumping rate( P ＜ 0. 05) . Atr-Mor and Ani-Mor had a weak effect on the elevation of the pain threshold of Mor-dependent mice, but had strong effects on the decrease of the jumping times and the jumping rate( P ＜ 0. 01 ).CONCLUSION: Belladonna alkaloids all could antagonize Mor-dependence in mice at different degrees, which provide an important experimental evidence to develop belladonna drugs for preventing opium addiction.