CAJ | 학술논문

目的研究幽门螺杆菌（H elicobacter py lori,H py lori）简易分离培养及保存方法,构建H py lori小型菌库。方法首先采用H py lori选择培养基,以烛缸法分离培养不同来源的标本,然后以革兰染色显微镜形态检查及生化试验鉴定为H py lori,最后把鉴定为H py lori的纯培养物,分别置于布氏肉汤、50%甘油血清肉汤、50%甘油蛋清3种不同的保存液,-20°C保存,并在保存后1、6、12 d分别检测其存活率。结果培养58份临床胃镜活检标本,获13株H py lori;56名在校大学生口腔和粪便标本,获8株H py lori;共获21株H py lo i,经布氏肉汤、50%甘油血清肉汤、50%甘油蛋清3种保存液-20°C保存,21株H py lori存活率在1 d和6 d时,3者均大于90%,统计学无显著差异;12 d时,50%甘油血清肉汤存活率大于80%,布氏肉汤和50%甘油蛋清均低于50%,差异有统计学意义。结论采用烛缸法可以成功分离培养H py lori,以50%甘油血清肉汤-20°CH py lori可存活12 d以上,两者结合可以在一般实验室构建简易的过渡性H py lori小型菌库,方便H py lori菌种的使用。
목적연구유문라간균（H elicobacter py lori,H py lori）간역분리배양급보존방법,구건H py lori소형균고。방법수선채용H py lori선택배양기,이충항법분리배양불동래원적표본,연후이혁란염색현미경형태검사급생화시험감정위H py lori,최후파감정위H py lori적순배양물,분별치우포씨육탕、50%감유혈청육탕、50%감유단청3충불동적보존액,-20°C보존,병재보존후1、6、12 d분별검측기존활솔。결과배양58빈림상위경활검표본,획13주H py lori;56명재교대학생구강화분편표본,획8주H py lori;공획21주H py lo i,경포씨육탕、50%감유혈청육탕、50%감유단청3충보존액-20°C보존,21주H py lori존활솔재1 d화6 d시,3자균대우90%,통계학무현저차이;12 d시,50%감유혈청육탕존활솔대우80%,포씨육탕화50%감유단청균저우50%,차이유통계학의의。결론채용충항법가이성공분리배양H py lori,이50%감유혈청육탕-20°CH py lori가존활12 d이상,량자결합가이재일반실험실구건간역적과도성H py lori소형균고,방편H py lori균충적사용。
Objects To study the simple method for the separation and reservation of Helicobacter pylori（H pylori） and construct a small storage of H pylori.Method Firstly,the candle jar method was used to separate samples of H pylori from different sources by H pylori selective medium,Secondarily,the Gram microscopy test and biochemical experiment were used to identify H pylori.Thirdly,the pure H pylori was reserved in three different preservation solutions（brucella broth,50% glycerol serum,50% glycerol egg white） which were 20 °C below zero.Finally,the samples were tested to find out the survival rate after 1-,6-,12-day reservation.Result Among the 21 H pylori samples,of which 13 were collected and obtained from 58 clinical specimens and the other 8 were from oral and fecal samples of 56 undergraduates,the survival rate of 21 of the samples was over 90%.That is,the survival rate showed no significant difference when they were tested after 1-and 6-day reservation.The survival rate of the samples reserved in different solutions showed significant different when tested in 12-day reservation： that of those in 50% glycerol serum was over 80%,that of those in the other solutions was less than 50%.Conclusion Candle jar method can be used as a good way to isolated H pylori and the 50% glycerol which is 20 °C below zero can reserve H pylori well for more than 12 days.It is convenient to construct a simple and small storage of H pylori and valuable in scientific research by using the bacteria from the storage.