CAJ | 학술논문

[目的] 研究不育枸杞花药营养物质代谢与花粉败育的关系,为枸杞不育系花粉败育机制研究提供理论依据.[方法] 运用半薄切片和细胞化学染色技术,分别进行多糖(含淀粉粒)、脂类和蛋白质的染色鉴定,对枸杞不育系和可育系花药营养物质积累和分布状况进行观察和比较分析.[结果] 不育系与可育系花药半薄切片经组织化学染色后,多糖PAS反应呈阳性,红色,淀粉粒为红色颗粒.脂类苏丹黑B染色呈阳性,黑色颗粒.蛋白质考马斯亮蓝染色呈阴性.表明枸杞不育系与可育系一样,花药发育过程中积累储存的营养物质是淀粉粒和脂滴,没有储藏形式的蛋白质.与可育系相比,不育系花药减数分裂后,淀粉粒在药隔薄壁组织中骤减,表皮和药室内壁中的淀粉粒也大幅减少,绒毡层没有脂质积累;绒毡层和四分孢子先后发生液泡化、进入解体过程.[结论] 可育系药隔维管束多糖营养供应充足,绒毡层细胞多糖转化、合成等活动旺盛.而不育系药隔多糖供应在减数分裂后急剧减少,使得绒毡层细胞多糖含量减少,导致绒毡层细胞多糖转化、合成脂质等营养物质的机制发生紊乱.由此引发了绒毡层提前进入细胞程序性死亡过程,四分孢子因营养匮乏,最终解体.
[목적] 연구불육구기화약영양물질대사여화분패육적관계,위구기불육계화분패육궤제연구제공이론의거.[방법] 운용반박절편화세포화학염색기술,분별진행다당(함정분립)、지류화단백질적염색감정,대구기불육계화가육계화약영양물질적루화분포상황진행관찰화비교분석.[결과] 불육계여가육계화약반박절편경조직화학염색후,다당PAS반응정양성,홍색,정분립위홍색과립.지류소단흑B염색정양성,흑색과립.단백질고마사량람염색정음성.표명구기불육계여가육계일양,화약발육과정중적루저존적영양물질시정분립화지적,몰유저장형식적단백질.여가육계상비,불육계화약감수분렬후,정분립재약격박벽조직중취감,표피화약실내벽중적정분립야대폭감소,융전층몰유지질적루;융전층화사분포자선후발생액포화、진입해체과정.[결론] 가육계약격유관속다당영양공응충족,융전층세포다당전화、합성등활동왕성.이불육계약격다당공응재감수분렬후급극감소,사득융전층세포다당함량감소,도치융전층세포다당전화、합성지질등영양물질적궤제발생문란.유차인발료융전층제전진입세포정서성사망과정,사분포자인영양궤핍,최종해체.
[Objective] This study deals with the relation between anther nutrient metabolism and pollen abortion of male sterile lines in Lycium barbarum L., and provides some theoretical references for the pollen abortion mechanism of male sterile lines in Lycium barbarum L.. [Method] By using semi-thin section and cytochemistry technology, the accumulation and distribution of anther nutrient of male sterile and fertile lines in Lycium barbarum L. were observed and compared. [Result] The result showed that after meiosis, starch grains in the connective parenchyma of anther decreased sharply, and starch grains in epidermis and endothecium were also greatly decreased, while nearly no lipid accumulated in tapetal cells in male sterile lines compared with the fertile lines. The tapetal cells and tetraspore had vacuoles appear successively, and then entered the degenerate process. During the degeneration stage of tapetal cells and tetraspore, starch grains in epidermis and endothecium were still in a small number and in the connective parenchyma as well. [Conclusion] The decrease of polysaccharide supplying in anther vascular leads to the disorder of mechanism of glycolipid transforming in tapetal cells, which triggers the tapetal cells degenerating ahead of time, and the latter gives rise to callus wall failing to degenerate in time. Therefore, the tetrads disintegrate and disappear ultimately due to lack of nutrition.