CAJ | 학술논문

为了探索昆虫取食诱导的木本植物体内所产生的防御反应,以合作杨(Populus simonii×P.pyramidalis,'Opera 8277')扦插苗为实验材料,经杨扇舟蛾(Clostera anachoreta)幼虫取食后,检测叶片中茉莉酸(jasmonate,JA)途径中的关键酶--脂氧合酶(lipoxygenas,LOX)及苯基丙酸类合成途径中的限速酶--苯丙氨酸解氨酶(phenylalanine ammonia lyase,PAL)的活性变化.结果显示,LOX和PAL的活性不仅在虫咬叶片中出现增加,在虫咬叶片上部的系统叶片中也有显著升高,表明茉莉酸途径和苯基丙酸类合成途径被激活,而且防御反应被系统性诱导.并且,与虫咬植株邻近的健康杨树叶片中LOX和PAL的增加表明,杨树间存在由昆虫取食诱导挥发物介导的信息传递.熏蒸实验也证明,茉莉酸甲酯(methyl jasmonate,MeJA)能够作为气体信号诱导合作杨植株产生防御反应.
위료탐색곤충취식유도적목본식물체내소산생적방어반응,이합작양(Populus simonii×P.pyramidalis,'Opera 8277')천삽묘위실험재료,경양선주아(Clostera anachoreta)유충취식후,검측협편중말리산(jasmonate,JA)도경중적관건매--지양합매(lipoxygenas,LOX)급분기병산류합성도경중적한속매--분병안산해안매(phenylalanine ammonia lyase,PAL)적활성변화.결과현시,LOX화PAL적활성불부재충교협편중출현증가,재충교협편상부적계통협편중야유현저승고,표명말리산도경화분기병산류합성도경피격활,이차방어반응피계통성유도.병차,여충교식주린근적건강양수협편중LOX화PAL적증가표명,양수간존재유곤충취식유도휘발물개도적신식전체.훈증실험야증명,말리산갑지(methyl jasmonate,MeJA)능구작위기체신호유도합작양식주산생방어반응.
A study was conducted to explore the defense response in woody plants after insect herbivory. The activities of two enzymes, lipoxygenase (LOX), a key enzyme of jasmonate (JA) pathway, and phenylalanine ammonia lyase (PAL), a rate-limiting enzyme of phenyl-propanoid pathway, were measured in the leaves of one-year-old poplar (Populus simonii × P. pyramidalis 'Opera 8277') cuttings after Clo-stera anachoreta larvae attack. The results show that the increased activities of LOX and PAL were found not only in the leaves wounded by C. anachoreta larvae but also in their upper systemic leaves, indicating that JA and phenylpropanoid pathways were activated, and the de-fense response was stimulated systemically. The increase in LOX and PAL activities in neighboring intact poplar cuttings suggested that there exists the interplant communication between poplar plants mediated by the herbivore-induced volatiles. Methyl jasmonate (MeJA) was also proved to be an airborne signal to induce defense response in P. simonii × P. pyramidalis ‘Opera 8277' cuttings.