CAJ | 학술논문

[目的]获得珠颈斑鸠(Streptopelia chinensis)线粒体12S rRNA基因序列,并以此分析不同鸟类的分子进化关系.[方法]根据其他鸟类线粒体12S rRNA基因及侧翼序列设计引物,采用PCR的方法获得珠颈斑鸠线粒体12S rRNA基因片段,克隆后进行序列测定,利用Mega 4.0软件Neighbor-Joining法分析不同鸟类的进化关系.[结果]获得珠颈斑鸠线粒体12S rRNA基因序列长度为970 bp,碱基组成为:A=31.6%、C=28.9%、G=19.8%、T=19.7%,其中A+T含量高于G+C含量.通过与GenBank中环颈斑鸠(Streptopelia capicola)等其他8种鸟类12S rRNA基因序列比较发现,珠颈斑鸠与环颈斑鸠的同源性最高,为94.4%,与家鸭(Anas platyrhynchos)的同源性最低,为78.4%.并根据这9种鸟类序列用NJ法构建进化树,结果表明,珠颈斑鸠和其他8种鸟类在分子水平上反映的进化关系与在形态学上的进化关系基本吻合.[结论]首次得到珠颈斑鸠12S rRNA基因全长序列,并确定了珠颈斑鸠与其他8种鸟类的分子进化关系.
[목적]획득주경반구(Streptopelia chinensis)선립체12S rRNA기인서렬,병이차분석불동조류적분자진화관계.[방법]근거기타조류선립체12S rRNA기인급측익서렬설계인물,채용PCR적방법획득주경반구선립체12S rRNA기인편단,극륭후진행서렬측정,이용Mega 4.0연건Neighbor-Joining법분석불동조류적진화관계.[결과]획득주경반구선립체12S rRNA기인서렬장도위970 bp,감기조성위:A=31.6%、C=28.9%、G=19.8%、T=19.7%,기중A+T함량고우G+C함량.통과여GenBank중배경반구(Streptopelia capicola)등기타8충조류12S rRNA기인서렬비교발현,주경반구여배경반구적동원성최고,위94.4%,여가압(Anas platyrhynchos)적동원성최저,위78.4%.병근거저9충조류서렬용NJ법구건진화수,결과표명,주경반구화기타8충조류재분자수평상반영적진화관계여재형태학상적진화관계기본문합.[결론]수차득도주경반구12S rRNA기인전장서렬,병학정료주경반구여기타8충조류적분자진화관계.
[Objective] This research aimed to obtain the sequence of 12S rRNA gene of Streptopelia chinensis and reconstruct the phylogeny of 9 species of birds based on 12S rRNA sequence.[Method] The primers were designed based on 12S rRNA gene and flank sequence of other species of birds.Fragments were amplified by PCR and cloned into plasmid,and then sequenced.The phylogenetic tree of 9 species of birds was reconstructed using NJ method by Mega 4.0.[Result] The length of the obtained 12S rRNA gene of Streptopelia chinensis was 970 bp,the base composition was: A = 31.6%,C = 28.9%,G = 19.8%,T = 19.7%,respectively,and the A + T content was higher than G + C content.The 12S rRNA gene sequence of Streptopelia chinensis was aligned with Streptopelia capicola and other 7 species of birds on GenBank.The results showed that the sequence homology was highest at 94.4% between Streptopelia chinensis and Streptopelia capicola,and lowest at 79.8% between Streptopelia chinensis and Anas platyrhynchos.A phylogenetic tree was constructed using the neighbor-joining method based on 12S rRNA gene sequence.According to the map analysis,the molecular phylogenetic relationships of Streptopelia chinensis with other eight species of birds were consistent with their morphologic phylogenetic relationships.[Conclusion] The entire sequence of 12S rRNA gene of Streptopelia chinensis was successfully obtained.A phylogeny of Streptopelia chinensis and other 8 species of birds was reconstructed.