CAJ | 학술논문

目的:观察耐力训练大鼠心肌JAKs和SOCSs家族成员的表达,探讨耐力训练后JAK/STAT信号通路在心肌调节中的作用.方法:雄性SD大鼠进行10周递增负荷跑台训练后,采用免疫组化法观察末次运动后即刻和24小时心肌JAK1、JAK2、JAK3和SOCS1、SOCS2、SOCS6表达,计算阳性细胞率.结果:耐力训练大鼠运动后即刻心肌JAK1阳性细胞率显著高于运动后24小时组和安静对照组(P<0.01,P<0.05),JAK2阳性细胞率无论在运动后即刻还是运动后24小时均显著高于安静对照组(P<0.05,P<0.01),而JAK3阳性细胞率与安静对照组差异无统计学意义(P>0.05).耐力训练大鼠心肌SOCS1阳性细胞率在运动后即刻和运动后24小时均较安静对照组显著升高(P<0.01,P<0.05),SOCS2阳性细胞率也较安静对照组显著升高(P<0.05,P<0.05),而SOCS6阳性细胞率与安静对照组差异无统计学意义(P>0.05).结论:耐力训练诱发大鼠心肌JAK1、JAK2与JAK3表达变化趋势不同,JAK1、JAK2表达升高可能影响JAK/STAT信号通路对耐力训练大鼠心肌功能和形态的调节;而耐力训练后SOCS1、SOCS2表达显著升高提示其对心肌JAK/STAT信号通路的内源性负性调节作用加强.
목적:관찰내력훈련대서심기JAKs화SOCSs가족성원적표체,탐토내력훈련후JAK/STAT신호통로재심기조절중적작용.방법:웅성SD대서진행10주체증부하포태훈련후,채용면역조화법관찰말차운동후즉각화24소시심기JAK1、JAK2、JAK3화SOCS1、SOCS2、SOCS6표체,계산양성세포솔.결과:내력훈련대서운동후즉각심기JAK1양성세포솔현저고우운동후24소시조화안정대조조(P<0.01,P<0.05),JAK2양성세포솔무론재운동후즉각환시운동후24소시균현저고우안정대조조(P<0.05,P<0.01),이JAK3양성세포솔여안정대조조차이무통계학의의(P>0.05).내력훈련대서심기SOCS1양성세포솔재운동후즉각화운동후24소시균교안정대조조현저승고(P<0.01,P<0.05),SOCS2양성세포솔야교안정대조조현저승고(P<0.05,P<0.05),이SOCS6양성세포솔여안정대조조차이무통계학의의(P>0.05).결론:내력훈련유발대서심기JAK1、JAK2여JAK3표체변화추세불동,JAK1、JAK2표체승고가능영향JAK/STAT신호통로대내력훈련대서심기공능화형태적조절;이내력훈련후SOCS1、SOCS2표체현저승고제시기대심기JAK/STAT신호통로적내원성부성조절작용가강.
Objective To evaluate Janus kinase (JAKs) and Suppressors of cytokine signaling(SOCSs) expression in myocardium after endurance training. Methods 30 male Sprague-Dawley rats undertook 10 weeks grade treadmill training program and were sacrificed at 0 and 24 h respectively after the last exercise. JAK1,JAK2,JAK3 and SOCS1 ,SOCS2,SOCS6 expression in the myocardium were determined by im munohistochemistry method. Results The percentage of JAK1 positive cell significantly increased immediately after last running and recovered to the sedentary level 24 hrs after exercise;The percentage of JAK2 positive cell significantly increased immediately after last running and continuously at a higher level 24 hrs after exercise (P<0.01;P<0.05); JAK.3 showed no significant change either immediately or 24 hrs after exercise (P>0.05). The percentage of SOCS1 and SOCS2 positive cell also significantly increased both immediately and 24 hrs after exercise (P<0.01 ;P<0.05). However, the percentage of SOCS6 positive cell showed no significant change either immediately or 24 hrs after exercise(P>0.05). Conclusion Endurance training could induce different responses of JAK1,JAK2 and JAK3. The increased expressions of JAK1 and JAK2 might indicate the changes of JAK/STAT signal pathway in regulating myocardial function. The increased expressions of SOCS1 and SOCS2 after endurance training might indicate the increased exertion in regulating JAK/STAT signal pathway in cardiac muscle.