中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2010年
1期
1-4
,共4页
刘汉臣%李雨%李百龙%崔建国%高福%孙顶%单鹄声%赵芳%黄越承%董俊瑞%刘浩%蔡建明
劉漢臣%李雨%李百龍%崔建國%高福%孫頂%單鵠聲%趙芳%黃越承%董俊瑞%劉浩%蔡建明
류한신%리우%리백룡%최건국%고복%손정%단곡성%조방%황월승%동준서%류호%채건명
羧酸富勒烯C_3%K562细胞%AHH-1细胞%γ射线%细胞凋亡
羧痠富勒烯C_3%K562細胞%AHH-1細胞%γ射線%細胞凋亡
최산부륵희C_3%K562세포%AHH-1세포%γ사선%세포조망
Carboxyfullerene C_3%K562 cell%AHH-1 cell%γ-ray%Apoptosis
目的 探讨羧酸富勒烯C_3(C_3)在辐射防护和辅助放疗方面的应用.方法 以不同浓度C_3与AHH-1、K562细胞共孵育,用CCK-8法、锥虫蓝试验检测C_3对细胞活力和存活率的影响,用Annexin-V/PI染色、流式细胞分析法研究照射后细胞凋亡和细胞周期的变化.结果 C_3对AHH-1细胞活力几乎无影响(存活率大于95%),但600 mg/L的C,使K562细胞存活率明显降低(82%).100 mg/L C,用药照射组与单纯照射组相比,4 Gy照射后AHH-1细胞的存活率显著升高(71.3%、90.3%),细胞凋亡率明显下降(26.3%、12.6%);而受照射24 Gy的K562细胞存活率却呈现下降趋势(69.4%、66.1%),不同剂量照射后细胞凋亡率反而增加.细胞周期分析结果显示,C,处理组AHH-1细胞12 Gy辐射后G_2期阻滞(27.2%)与单纯照射组(40.8%)相比减轻;而K562受照细胞则反而加重.结论 C_3对AHH-1细胞具有较好的辐射防护作用,而对K562细胞则表现为抑制细胞增殖,促进辐射诱导的细胞凋亡并加重G_2期阻滞.
目的 探討羧痠富勒烯C_3(C_3)在輻射防護和輔助放療方麵的應用.方法 以不同濃度C_3與AHH-1、K562細胞共孵育,用CCK-8法、錐蟲藍試驗檢測C_3對細胞活力和存活率的影響,用Annexin-V/PI染色、流式細胞分析法研究照射後細胞凋亡和細胞週期的變化.結果 C_3對AHH-1細胞活力幾乎無影響(存活率大于95%),但600 mg/L的C,使K562細胞存活率明顯降低(82%).100 mg/L C,用藥照射組與單純照射組相比,4 Gy照射後AHH-1細胞的存活率顯著升高(71.3%、90.3%),細胞凋亡率明顯下降(26.3%、12.6%);而受照射24 Gy的K562細胞存活率卻呈現下降趨勢(69.4%、66.1%),不同劑量照射後細胞凋亡率反而增加.細胞週期分析結果顯示,C,處理組AHH-1細胞12 Gy輻射後G_2期阻滯(27.2%)與單純照射組(40.8%)相比減輕;而K562受照細胞則反而加重.結論 C_3對AHH-1細胞具有較好的輻射防護作用,而對K562細胞則錶現為抑製細胞增殖,促進輻射誘導的細胞凋亡併加重G_2期阻滯.
목적 탐토최산부륵희C_3(C_3)재복사방호화보조방료방면적응용.방법 이불동농도C_3여AHH-1、K562세포공부육,용CCK-8법、추충람시험검측C_3대세포활력화존활솔적영향,용Annexin-V/PI염색、류식세포분석법연구조사후세포조망화세포주기적변화.결과 C_3대AHH-1세포활력궤호무영향(존활솔대우95%),단600 mg/L적C,사K562세포존활솔명현강저(82%).100 mg/L C,용약조사조여단순조사조상비,4 Gy조사후AHH-1세포적존활솔현저승고(71.3%、90.3%),세포조망솔명현하강(26.3%、12.6%);이수조사24 Gy적K562세포존활솔각정현하강추세(69.4%、66.1%),불동제량조사후세포조망솔반이증가.세포주기분석결과현시,C,처리조AHH-1세포12 Gy복사후G_2기조체(27.2%)여단순조사조(40.8%)상비감경;이K562수조세포칙반이가중.결론 C_3대AHH-1세포구유교호적복사방호작용,이대K562세포칙표현위억제세포증식,촉진복사유도적세포조망병가중G_2기조체.
Objective To investigate the application prospective of carboxyfullerene C_3 as a radioprotectant or assistant for tumor radiotherapy.Methods Different concentrations of C_3 were incubated with K562 and AHH-1 cell,CCK-8 assay and trypan blue rejection test were performed to examine the influence of C_3 on the cell viability.Annexin V/PI staining and flow cytometry assay were applied to assess the cell cycle and apoptosis after 7-ray irradiation.Results C_3 showed little toxicity to AHH-1 cell with the survival rate over 95% ,but 600 mg/L of C_3 markedly inhibited the growth of K562 cell (82%) .Pretreatment of 100 mg/L C_3 significantly increased the survival rate of AHH-1 cell after 4 Gy irradiation compared with the single radiation group(71.3% vs 90.3%) ,but decreased the apoptosis rate (26.3% vs 12.6%) ,while the survival rate of K562 cell was decreased and the apoptosis rate was elevated with the increase of C_3 concentration.Moreover,the cell cycle analysis revealed the G_2 phase block in AHH-1 cell after radiation exposure was mitigated by C_3 pretreatment,but that in K562 cell was aggravated.Conclusions C_3 has good radioprotective effects on AHH-1 cells.For K562 cell,C_3 could inhibit the cell proliferation,promote the radiation induced apoptosis and aggravate the G_2 phase block.
