中国行为医学科学
中國行為醫學科學
중국행위의학과학
2008年
9期
772-774
,共3页
周建松%李凌江%曹霞%李卫晖%李则宣
週建鬆%李凌江%曹霞%李衛暉%李則宣
주건송%리릉강%조하%리위휘%리칙선
应激%适应,心理学%神经递质%色普法,高效液相
應激%適應,心理學%神經遞質%色普法,高效液相
응격%괄응,심이학%신경체질%색보법,고효액상
Stress%Adaptation,psychological%Neurotransmitter%Chromatography,high pressure liquid
目的 研究单次及重复应激对大鼠行为及部分脑区单胺类神经递质及其代谢产物的影响.方法 采用限制应激模型(6h·次-1·d-1,重复应激连续10d).采用空场试验、高架十字迷宫试验评估大鼠焦虑水平,高效液相色谱法检测大鼠前额叶、海马和下丘脑的单胺类神经递质.结果 与对照组比较,单次应激组在空场实验中央格停留时间少(P<0.01),在高架十字迷宫实验闭臂停留时间多(P<0.01)、开臂停留时间少(P<0.01);重复应激组在中央格停留时间、在开/闭臂停留时间与对照组差异有显著性(P>0.05).递质分析表明,与对照组前额叶5-羟色胺[(245.84 4-31.32)ng/g]、5-羟吲哚乙酸[(200.69±38.47)ng/g]比较,单次应激大鼠前额叶[(314.05±31.31)ng/g,(286.32±30.10)ng/g]水平较高(P<0.01);与对照组海马[(182.16±16.48)ng/g,(284.13±63.86)ng/g]比较,单次应激组海马[(282.21±18.98)ng/g,(502.82±77.98)ng/g]水平较高(P<0.01);与对照组相比,重复应激组海马[(314.15±17.42)ng/g,(576.17±51.05)ng/g]较高(P<0.01).结论 大鼠单次应激产生明显的应激反应,而重复暴露于相同负性事件能够导致对应激的适应;海马5-HT释放的增加可能是应激适应的神经生物学基础.
目的 研究單次及重複應激對大鼠行為及部分腦區單胺類神經遞質及其代謝產物的影響.方法 採用限製應激模型(6h·次-1·d-1,重複應激連續10d).採用空場試驗、高架十字迷宮試驗評估大鼠焦慮水平,高效液相色譜法檢測大鼠前額葉、海馬和下丘腦的單胺類神經遞質.結果 與對照組比較,單次應激組在空場實驗中央格停留時間少(P<0.01),在高架十字迷宮實驗閉臂停留時間多(P<0.01)、開臂停留時間少(P<0.01);重複應激組在中央格停留時間、在開/閉臂停留時間與對照組差異有顯著性(P>0.05).遞質分析錶明,與對照組前額葉5-羥色胺[(245.84 4-31.32)ng/g]、5-羥吲哚乙痠[(200.69±38.47)ng/g]比較,單次應激大鼠前額葉[(314.05±31.31)ng/g,(286.32±30.10)ng/g]水平較高(P<0.01);與對照組海馬[(182.16±16.48)ng/g,(284.13±63.86)ng/g]比較,單次應激組海馬[(282.21±18.98)ng/g,(502.82±77.98)ng/g]水平較高(P<0.01);與對照組相比,重複應激組海馬[(314.15±17.42)ng/g,(576.17±51.05)ng/g]較高(P<0.01).結論 大鼠單次應激產生明顯的應激反應,而重複暴露于相同負性事件能夠導緻對應激的適應;海馬5-HT釋放的增加可能是應激適應的神經生物學基礎.
목적 연구단차급중복응격대대서행위급부분뇌구단알류신경체질급기대사산물적영향.방법 채용한제응격모형(6h·차-1·d-1,중복응격련속10d).채용공장시험、고가십자미궁시험평고대서초필수평,고효액상색보법검측대서전액협、해마화하구뇌적단알류신경체질.결과 여대조조비교,단차응격조재공장실험중앙격정류시간소(P<0.01),재고가십자미궁실험폐비정류시간다(P<0.01)、개비정류시간소(P<0.01);중복응격조재중앙격정류시간、재개/폐비정류시간여대조조차이유현저성(P>0.05).체질분석표명,여대조조전액협5-간색알[(245.84 4-31.32)ng/g]、5-간신타을산[(200.69±38.47)ng/g]비교,단차응격대서전액협[(314.05±31.31)ng/g,(286.32±30.10)ng/g]수평교고(P<0.01);여대조조해마[(182.16±16.48)ng/g,(284.13±63.86)ng/g]비교,단차응격조해마[(282.21±18.98)ng/g,(502.82±77.98)ng/g]수평교고(P<0.01);여대조조상비,중복응격조해마[(314.15±17.42)ng/g,(576.17±51.05)ng/g]교고(P<0.01).결론 대서단차응격산생명현적응격반응,이중복폭로우상동부성사건능구도치대응격적괄응;해마5-HT석방적증가가능시응격괄응적신경생물학기출.
Objective To investigate the effect of single or repeated restraint stress on the behavior and the brain monoamines neurotransmitters in rats.Methods The behavior were observed by the Open Field test and the Elevated Plus-Maze test,the monoamines neurotransmitters and their metabolites in homogenates obtained from the frontal cortex,hippocampus and hypothalamus were assessed by HPLC.Results The single stressed group showed more anxiety behaviors in the Open Field test and the Elevated Plus-Maze test than the control,while the repeated stressed group did not.In the single streased group,the level of 5-HT and 5-HIAA in the frontal cortex(5-HT:314.05±31.31,5-HIAA:286.32±30.10)and hippocampus(5-HT:282.21±18.98,5-HIAA:2.82±77.98)were significantly higher than that of the control(frontal cortex:5-HT:245.84±31.32,5-HIAA:200.69±38.47;hippocampus:5-HT:182.16±16.48,5-HIAA:284.13±63.86)(P＜0.01).In the repeated stressed group,the level of 5-HT and 5-HIAA in the hippocampus was significantly higher than that of the control(P<0.01).Conclusion This study indicated that single stress induce the anxiety behavior,repeat exposed the same adverse event can make the rats adaptation to this kind of stress.The single and repeated stress can increase the release of the 5-HT in the hippocampus,which may be the critical neurobiology of the stress adaptation.
