中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2011年
12期
1023-1026
,共4页
薛迪新%陈积贤%任约翰%吴伟力%张仁虎%林道浙%余铭%林肖%梁美珍%徐平
薛迪新%陳積賢%任約翰%吳偉力%張仁虎%林道浙%餘銘%林肖%樑美珍%徐平
설적신%진적현%임약한%오위력%장인호%림도절%여명%림초%량미진%서평
结直肠肿瘤%微FRNAs%基因表达%病理学,临床
結直腸腫瘤%微FRNAs%基因錶達%病理學,臨床
결직장종류%미FRNAs%기인표체%병이학,림상
Colorectal neoplasms%microRNAs%Gene expression%Pathology,clinical
目的 探讨miR-21和PDCD4mRNA在大肠癌组织中的表达及与其临床病理特征的关系,并阐明在大肠癌活体组织中miR-21与其靶基因PDCD4表达的关系.方法 用荧光定量PCR技术检测43对大肠癌组织及其对应的正常黏膜组织中的miR-21和PDCD4 mRNA表达量；免疫组化SP法检测PDCD4蛋白的表达.结果 大肠癌组织中miR-21的表达升高(P＜0.05),而PDCD4mRNA的表达降低(P＜0.05).miR-21的表达与大肠癌的淋巴结转移、临床分期(Ⅰ+Ⅱ、Ⅲ+Ⅳ)有关(P＜0.05),而与患者的性别、肿瘤位置、肿瘤大小、浸润深度、远处转移、临床分期(Ⅰ、Ⅱ)无关(P＞0.05)；PDCD4mRNA的表达与大肠癌的浸润深度、临床分期(Ⅰ、Ⅱ)有关(P＜0.05),而与患者的性别、肿瘤位置、肿瘤大小、淋巴结转移、远处转移、临床分期(Ⅰ+Ⅱ、Ⅲ+Ⅳ)无关(P＞0.05).miR-21的表达与PDCD4蛋白的核表达、核与浆表达之和存在负相关(P＜0.05),而与PDCD4mRNA、PDCD4蛋白的浆表达无相关性(P＞0.05).结论 miR-21和PDCD4mRNA在大肠癌中的异常表达与大肠癌的预后有关；在大肠癌活体组织中,miR-21通过与PDCD4mRNA的3’非编码区结合抑制PDCD4mRNA翻译成蛋白质.
目的 探討miR-21和PDCD4mRNA在大腸癌組織中的錶達及與其臨床病理特徵的關繫,併闡明在大腸癌活體組織中miR-21與其靶基因PDCD4錶達的關繫.方法 用熒光定量PCR技術檢測43對大腸癌組織及其對應的正常黏膜組織中的miR-21和PDCD4 mRNA錶達量；免疫組化SP法檢測PDCD4蛋白的錶達.結果 大腸癌組織中miR-21的錶達升高(P＜0.05),而PDCD4mRNA的錶達降低(P＜0.05).miR-21的錶達與大腸癌的淋巴結轉移、臨床分期(Ⅰ+Ⅱ、Ⅲ+Ⅳ)有關(P＜0.05),而與患者的性彆、腫瘤位置、腫瘤大小、浸潤深度、遠處轉移、臨床分期(Ⅰ、Ⅱ)無關(P＞0.05)；PDCD4mRNA的錶達與大腸癌的浸潤深度、臨床分期(Ⅰ、Ⅱ)有關(P＜0.05),而與患者的性彆、腫瘤位置、腫瘤大小、淋巴結轉移、遠處轉移、臨床分期(Ⅰ+Ⅱ、Ⅲ+Ⅳ)無關(P＞0.05).miR-21的錶達與PDCD4蛋白的覈錶達、覈與漿錶達之和存在負相關(P＜0.05),而與PDCD4mRNA、PDCD4蛋白的漿錶達無相關性(P＞0.05).結論 miR-21和PDCD4mRNA在大腸癌中的異常錶達與大腸癌的預後有關；在大腸癌活體組織中,miR-21通過與PDCD4mRNA的3’非編碼區結閤抑製PDCD4mRNA翻譯成蛋白質.
목적 탐토miR-21화PDCD4mRNA재대장암조직중적표체급여기림상병리특정적관계,병천명재대장암활체조직중miR-21여기파기인PDCD4표체적관계.방법 용형광정량PCR기술검측43대대장암조직급기대응적정상점막조직중적miR-21화PDCD4 mRNA표체량；면역조화SP법검측PDCD4단백적표체.결과 대장암조직중miR-21적표체승고(P＜0.05),이PDCD4mRNA적표체강저(P＜0.05).miR-21적표체여대장암적림파결전이、림상분기(Ⅰ+Ⅱ、Ⅲ+Ⅳ)유관(P＜0.05),이여환자적성별、종류위치、종류대소、침윤심도、원처전이、림상분기(Ⅰ、Ⅱ)무관(P＞0.05)；PDCD4mRNA적표체여대장암적침윤심도、림상분기(Ⅰ、Ⅱ)유관(P＜0.05),이여환자적성별、종류위치、종류대소、림파결전이、원처전이、림상분기(Ⅰ+Ⅱ、Ⅲ+Ⅳ)무관(P＞0.05).miR-21적표체여PDCD4단백적핵표체、핵여장표체지화존재부상관(P＜0.05),이여PDCD4mRNA、PDCD4단백적장표체무상관성(P＞0.05).결론 miR-21화PDCD4mRNA재대장암중적이상표체여대장암적예후유관；재대장암활체조직중,miR-21통과여PDCD4mRNA적3’비편마구결합억제PDCD4mRNA번역성단백질.
Objective To investigate the expression of miR-21 and PDCD4mRNA in colorectal cancer and their correlation with clinicopathological characteristics,to elucidate relationship between PDCD4and miR-21 in vivo.Methods Expression of RNA (including miR-21and PDCD4mRNA) and PDCD4 protein were detected respectively by quantitative real-time reverse transcriptase-PCR and SP immunohistochemical staining in 43 cases of colorectal carcinoma and corresponding normal mucous membrane tissue.Results In colorectal carcinoma,expression of miR-21 was higher than that of the control ( P ＜ 0.05 ),and expression of PDCD4mRNA lower than the control ( P ＜ 0.05 ).Expression of miR21 was associated with lymph node metastasis and clinical stages ( Ⅰ + Ⅱ,Ⅲ + Ⅳ ) ( P ＜ 0.05 ).On the other hand,no significant differences were observed regarding sex,tumor site,size,local invasion,distant metastases,clinical stages( Ⅰ,Ⅱ ) (P ＞ 0.05 ).Expression of PDCD4mRNA was associated with local invasion,clinical stages ( Ⅰ,Ⅱ ) ( P ＜ 0.05 ).While no significant differences were found concerning sex,site,size,lymph node metastasis,distant metastases,clinical stages( Ⅰ + Ⅱ,Ⅲ + Ⅳ ) ( P ＞ 0.05 ).miR21 levels was negatively correlated with PDCD4 expression including nuclear and nuclear and cytoplasmic put together (P ＜ 0.05 ),in contrast to PDCD4mRNA and PDCD4 expression in cytoplasmic ( P ＞ 0.05 ).Conclusions ( 1 ) Abnormal expression of PDCD4 mRNA and miR-21 correlate with prognosis in colorectal cancer.(2) miR-21 suppress translation of PDCD4mRNA by binding to the 3'-untranslated region (3'-UTR)of target PDCD4mRNA.
