国际免疫学杂志
國際免疫學雜誌
국제면역학잡지
INTERNATIONAL JOURNAL OF IMMUNOLOGY
2010年
3期
245-248
,共4页
樊有龙%吕娇凤%晏萍兰%房向东
樊有龍%呂嬌鳳%晏萍蘭%房嚮東
번유룡%려교봉%안평란%방향동
类风湿关节炎%白细胞介素-17%血管内皮生长因子%抗血管内皮细胞抗体
類風濕關節炎%白細胞介素-17%血管內皮生長因子%抗血管內皮細胞抗體
류풍습관절염%백세포개소-17%혈관내피생장인자%항혈관내피세포항체
Rheumatoid arthritis%Interleukin-17%Vascular endothelial growth factor Anti endothelial cell antibodies
目的 通过对类风湿关节炎(RA)患者血清抗血管内皮细胞抗体(AECA)、血浆血管内皮生长因子(VEGF)和白细胞介素-17(IL-17)的检测,旨在探讨AECA、VEGF、IL-17在RA患者发病、病情进展中的相关性及其内在联系.方法 采用间接免疫荧光法(IIF)和双抗体夹心酶联免疫吸附试验(ELISA)法检测86例RA、45例骨关节炎(OA)、30例健康对照AECA的阳性率和VEGF、IL-17水平,VEGF、IL-17水平与红细胞沉降率(ESR)、超敏C反应蛋白(hs-CRP)、类风湿因子(RF)等指标进行相关性分析.结果 RA患者AECA阳性率为8.1%,高于OA患者的阳性率2.2%(t:2.133,P<0.05)和健康对照的阳性率0(t=2.562,P<0.05);RA活动期AECA阳性率为16.7%,高于RA缓解期的阳性率3.6%(t=2.105,P<0.05);RA患者血浆IL-17和VEGF水平显著高于OA组(t=2.02、t=2.106,P<0.05)和健康对照组(t=2.413、t=2.469,P<0.05);RA患者活动期血浆IL-17和VEGF水平明显高于RA缓解组(t=2.315、t=2.232,P<0.05)及健康对照组(t:2.985、t=2.753,P<0.01);RA缓解组与健康对照组无明显差异(t=1.475、t=1.326,P>0.05);RA患者AECA滴度、血浆IL-17、VEGF水平与ESR、hs-CRP、RF的指标呈正相关.结论 AECA、VEGF、IL-17三者与RA的发病、病情活动存在一定的关系,IL-17、VEGF水平变化及AECA的滴度可作为临床观察RA病情活动、判断疗效及预后等方面的参考指标.
目的 通過對類風濕關節炎(RA)患者血清抗血管內皮細胞抗體(AECA)、血漿血管內皮生長因子(VEGF)和白細胞介素-17(IL-17)的檢測,旨在探討AECA、VEGF、IL-17在RA患者髮病、病情進展中的相關性及其內在聯繫.方法 採用間接免疫熒光法(IIF)和雙抗體夾心酶聯免疫吸附試驗(ELISA)法檢測86例RA、45例骨關節炎(OA)、30例健康對照AECA的暘性率和VEGF、IL-17水平,VEGF、IL-17水平與紅細胞沉降率(ESR)、超敏C反應蛋白(hs-CRP)、類風濕因子(RF)等指標進行相關性分析.結果 RA患者AECA暘性率為8.1%,高于OA患者的暘性率2.2%(t:2.133,P<0.05)和健康對照的暘性率0(t=2.562,P<0.05);RA活動期AECA暘性率為16.7%,高于RA緩解期的暘性率3.6%(t=2.105,P<0.05);RA患者血漿IL-17和VEGF水平顯著高于OA組(t=2.02、t=2.106,P<0.05)和健康對照組(t=2.413、t=2.469,P<0.05);RA患者活動期血漿IL-17和VEGF水平明顯高于RA緩解組(t=2.315、t=2.232,P<0.05)及健康對照組(t:2.985、t=2.753,P<0.01);RA緩解組與健康對照組無明顯差異(t=1.475、t=1.326,P>0.05);RA患者AECA滴度、血漿IL-17、VEGF水平與ESR、hs-CRP、RF的指標呈正相關.結論 AECA、VEGF、IL-17三者與RA的髮病、病情活動存在一定的關繫,IL-17、VEGF水平變化及AECA的滴度可作為臨床觀察RA病情活動、判斷療效及預後等方麵的參攷指標.
목적 통과대류풍습관절염(RA)환자혈청항혈관내피세포항체(AECA)、혈장혈관내피생장인자(VEGF)화백세포개소-17(IL-17)적검측,지재탐토AECA、VEGF、IL-17재RA환자발병、병정진전중적상관성급기내재련계.방법 채용간접면역형광법(IIF)화쌍항체협심매련면역흡부시험(ELISA)법검측86례RA、45례골관절염(OA)、30례건강대조AECA적양성솔화VEGF、IL-17수평,VEGF、IL-17수평여홍세포침강솔(ESR)、초민C반응단백(hs-CRP)、류풍습인자(RF)등지표진행상관성분석.결과 RA환자AECA양성솔위8.1%,고우OA환자적양성솔2.2%(t:2.133,P<0.05)화건강대조적양성솔0(t=2.562,P<0.05);RA활동기AECA양성솔위16.7%,고우RA완해기적양성솔3.6%(t=2.105,P<0.05);RA환자혈장IL-17화VEGF수평현저고우OA조(t=2.02、t=2.106,P<0.05)화건강대조조(t=2.413、t=2.469,P<0.05);RA환자활동기혈장IL-17화VEGF수평명현고우RA완해조(t=2.315、t=2.232,P<0.05)급건강대조조(t:2.985、t=2.753,P<0.01);RA완해조여건강대조조무명현차이(t=1.475、t=1.326,P>0.05);RA환자AECA적도、혈장IL-17、VEGF수평여ESR、hs-CRP、RF적지표정정상관.결론 AECA、VEGF、IL-17삼자여RA적발병、병정활동존재일정적관계,IL-17、VEGF수평변화급AECA적적도가작위림상관찰RA병정활동、판단료효급예후등방면적삼고지표.
Objective To.investigate the clinical significance of plasma anti endothelial cell antibodies (AECA) , vascular endothelial growth factor (VEGF) and interleukin-17 (IL-17) in patients with rheumatoid arthritis. Methods AECA, VEGF, and IL-17 were detected in 86 cases of rheumatoid arthritis ,45 cases of osteoarthritis (OA) and 30 healthy controls by indirect immunofluorescence(IIF) and by double antibody sandwich enzyme-linked immunosorbent assay(ELISA). The correlations among above results, erythrocyte sedimentation rate (ESR) , high-sensitivity C-reactive protein (hs-CRP) and rheumatoid factor (RF) were analyzed . Results The positive rate of AECA in patients with RA (8. 1%) was much higher than that in patients with OA(2.2%)(t=2. 133,P<0.05) and in the normal group(0) (t = 2. 562,P <0. 05). The positive rate of AECA in active RA patients (16.7%) was much higher than that of RA remission group(3.6%)(t =2. 105, P <0. 05). The plasma levels of IL-17 and VEGF of RA patients were significantly higher than that of OA patients (t=2.02,t=2. 106,P<0. 05) and the normal control (t =2. 413,t =2. 469,P <0. 05) ;Plasma levels of IL-17 and VEGF of active RA patients were significantly higher than that of RA remission group (t = 2. 315, t = 2. 232,P <0. 05) and healthy control group(t = 2. 985, t = 2. 753,P < 0. 01), but not significant different between that of RA remission group and healthy control group(t = 1. 475 ,t = 1. 326,P >0. 05) ;The titer of AECA, plasma IL-17 and VEGF of RA patients were positively correlated with ESR,hs-CRP,and RF. Conclu-sions AECA,VEGF and IL-17 are cor-related with the pathogenesis and activity of RA. They can be used as important indicators for the disease activity, therapeutic effect, and prognosis of patients with RA.
