中华小儿外科杂志
中華小兒外科雜誌
중화소인외과잡지
CHINESE JOURNAL OF PEDIATRIC SURGERY
2011年
8期
585-589
,共5页
李想%王珊%邓晓斌%马玉虹
李想%王珊%鄧曉斌%馬玉虹
리상%왕산%산효빈%마옥홍
卵黄囊瘤%SALL4%甲胎蛋白%免疫组织化学
卵黃囊瘤%SALL4%甲胎蛋白%免疫組織化學
란황낭류%SALL4%갑태단백%면역조직화학
Yolk sac tumor%SALL4%Alpha-fetoproteins%Immunohistochemistry
目的 研究分析一种新的肿瘤标志物SALL4在儿童卵黄囊瘤(yolk sac tumor,YST)的表达情况及临床意义.方法 收集儿童卵黄囊瘤病理标本21例(含有穿刺标本8例).选取成熟畸胎瘤(mature teratoma,MT)10例、未成熟畸胎瘤(immature teratoma,IMT)5例、无性细胞瘤1例进行对照研究.应用免疫组织化学方法检测SALL4在上述肿瘤中的表达情况,并就SALL4与甲胎蛋白(alpha fetoprotein,AFP)在YST的表达情况进行比较.结果 ①SALL4在21例儿童YST全部表达,且每例都有较高的阳性表达强度;②在儿童YST中,SALL4的阳性表达强度强,为6例(++)、15例(+++),而AFP在YST的表达相对较弱,分别为2例(-)、5例(+)、9例(++)、5例(+++),SALL4表达强度比AFP更强(P＜0.01);在儿童YST中,SALL4与AFP的表达强度之间进行相关性分析,发现两者无明显相关性(P＞0.05);③8例穿刺标本中,SALL4染色3例(++)、5例(+++),AFP染色1例(-)、2例(+)、4例(++)、1例(+++),两者比较差异有统计学意义(P＜0.05).结论 ①在儿童常见生殖细胞肿瘤(GCT)中,SALL4可能作为YST相对敏感的新一代瘤标;②在免疫组化染色分析中,SALL4比AFP在儿童YST中阳性表达强度更高;但SALL4与AFP的表达强度之间无明显相关性;③SALL4免疫组化染色可帮助儿童YST的诊断,尤其针对组织量少的穿刺标本,更敏感的瘤标SALL4有助于标本的病理分子学诊断.
目的 研究分析一種新的腫瘤標誌物SALL4在兒童卵黃囊瘤(yolk sac tumor,YST)的錶達情況及臨床意義.方法 收集兒童卵黃囊瘤病理標本21例(含有穿刺標本8例).選取成熟畸胎瘤(mature teratoma,MT)10例、未成熟畸胎瘤(immature teratoma,IMT)5例、無性細胞瘤1例進行對照研究.應用免疫組織化學方法檢測SALL4在上述腫瘤中的錶達情況,併就SALL4與甲胎蛋白(alpha fetoprotein,AFP)在YST的錶達情況進行比較.結果 ①SALL4在21例兒童YST全部錶達,且每例都有較高的暘性錶達彊度;②在兒童YST中,SALL4的暘性錶達彊度彊,為6例(++)、15例(+++),而AFP在YST的錶達相對較弱,分彆為2例(-)、5例(+)、9例(++)、5例(+++),SALL4錶達彊度比AFP更彊(P＜0.01);在兒童YST中,SALL4與AFP的錶達彊度之間進行相關性分析,髮現兩者無明顯相關性(P＞0.05);③8例穿刺標本中,SALL4染色3例(++)、5例(+++),AFP染色1例(-)、2例(+)、4例(++)、1例(+++),兩者比較差異有統計學意義(P＜0.05).結論 ①在兒童常見生殖細胞腫瘤(GCT)中,SALL4可能作為YST相對敏感的新一代瘤標;②在免疫組化染色分析中,SALL4比AFP在兒童YST中暘性錶達彊度更高;但SALL4與AFP的錶達彊度之間無明顯相關性;③SALL4免疫組化染色可幫助兒童YST的診斷,尤其針對組織量少的穿刺標本,更敏感的瘤標SALL4有助于標本的病理分子學診斷.
목적 연구분석일충신적종류표지물SALL4재인동란황낭류(yolk sac tumor,YST)적표체정황급림상의의.방법 수집인동란황낭류병리표본21례(함유천자표본8례).선취성숙기태류(mature teratoma,MT)10례、미성숙기태류(immature teratoma,IMT)5례、무성세포류1례진행대조연구.응용면역조직화학방법검측SALL4재상술종류중적표체정황,병취SALL4여갑태단백(alpha fetoprotein,AFP)재YST적표체정황진행비교.결과 ①SALL4재21례인동YST전부표체,차매례도유교고적양성표체강도;②재인동YST중,SALL4적양성표체강도강,위6례(++)、15례(+++),이AFP재YST적표체상대교약,분별위2례(-)、5례(+)、9례(++)、5례(+++),SALL4표체강도비AFP경강(P＜0.01);재인동YST중,SALL4여AFP적표체강도지간진행상관성분석,발현량자무명현상관성(P＞0.05);③8례천자표본중,SALL4염색3례(++)、5례(+++),AFP염색1례(-)、2례(+)、4례(++)、1례(+++),량자비교차이유통계학의의(P＜0.05).결론 ①재인동상견생식세포종류(GCT)중,SALL4가능작위YST상대민감적신일대류표;②재면역조화염색분석중,SALL4비AFP재인동YST중양성표체강도경고;단SALL4여AFP적표체강도지간무명현상관성;③SALL4면역조화염색가방조인동YST적진단,우기침대조직량소적천자표본,경민감적류표SALL4유조우표본적병리분자학진단.
Objective To study the expression of SALL4 in yolk sac tumors (YST) in children.Methods Twenty one patients were diagnosed with yolk sac tumor and treated in the children's hospital of Chongqing medical university. Their tumor tissue specimens including 8 puncture biopsy specimens were collected for this study. In addition, 10 cases of mature teratoma (MT), 5 cases of immature teratoma (IMT). 1 dysgerminoma were collected as control The expressions of SALL4 in YST,MT, IMT and dysgerminoma were studied by SP immunohistochemical method. Alpha fetoprotein 21 patients. Among them, the expression of SALL4 in YST was strong ( + + ) in 6 patients, and very It is negative in 2 cases, detectable in 5, strong in 9, and very strong in 5. The expression of SALL4 expression of SALL4 was strong ( + + ) in 3 cases, and very strong ( + + + ) in 5 cases. The staining of AFP was negative in 1 case ( - ), detectable in 2 ( + ), strong in 4 ( + + ) and very strong in 1 ( ++ + ). A significant difference was found between SALL4 and AFP expression in these specimens (P＜0. 05). Conclusions SALL4 could be a new relatively sensitive tumor marker for YST. The expression of SALL4 is stronger than AFP in YST. But their expression doesn't correlate with each other.Immunohistochemical staining of SALL4 could be helpful to diagnose YST in children, especially in the small amount specimens.
