CAJ | 학술논문

目的慢性不可预见性轻度应激大鼠抑郁模型检测阿魏酸钠的抗抑郁效果.方法 66只SD雄性大鼠(体质量220～280 g)随机分为对照组、慢性应激(模型)组、慢性应激+氟西汀(阳性对照)组和阿魏酸钠治疗(慢性应激+不同剂量阿魏酸钠)组,每组11只动物.长期不可预见性轻度应激+孤养复制大鼠慢性抑郁模型;阿魏酸钠腹腔注射(20mg·kg-1·d-1、40mg·kg-1·d-1、80mg·kg-1·d-1),持续28日.慢性应激期间测定动物的体质量和摄食量;开野、液体消耗试验检查动物行为;实验末行强迫游泳和组织病理学检查分析动物行为和海马组织形态学.结果成功建立慢性应激大鼠抑郁模型.阿魏酸钠治疗能部分逆转应激引起的动物的抑郁样表现.实验第4周末,阿魏酸钠治疗组(80mg/kg)大鼠的体质量增加量、糖水消耗量、运动和探究能力[分别为(88.06±7.83)g,(66.33±3.03)g,(25.10±4.90)分,(6.60±1.20)分,]均明显高于模型组[分别为(77.15±14.66)g,(40.75±6.05)g,(18.20±2.00)分,(3.20±0.60)分],强迫游泳不动时间(117.30±7.22)s则短于模型组(148.10±8.25)s,差异有显著性(P值分别为0.0425,0.0087,0.0409,0.0317,0.0025);阿魏酸钠治疗还能部分逆转应激引起的动物海马区神经元轻度水肿、变性.结论阿魏酸钠对慢性应激大鼠抑郁模型有明显的抗抑郁作用.
목적 만성불가예견성경도응격대서억욱모형검측아위산납적항억욱효과.방법 66지SD웅성대서(체질량220～280 g)수궤분위대조조、만성응격(모형)조、만성응격+불서정(양성대조)조화아위산납치료(만성응격+불동제량아위산납)조,매조11지동물.장기불가예견성경도응격+고양복제대서만성억욱모형;아위산납복강주사(20mg·kg-1·d-1、40mg·kg-1·d-1、80mg·kg-1·d-1),지속28일.만성응격기간측정동물적체질량화섭식량;개야、액체소모시험검사동물행위;실험말행강박유영화조직병이학검사분석동물행위화해마조직형태학.결과 성공건립만성응격대서억욱모형.아위산납치료능부분역전응격인기적동물적억욱양표현.실험제4주말,아위산납치료조(80mg/kg)대서적체질량증가량、당수소모량、운동화탐구능력[분별위(88.06±7.83)g,(66.33±3.03)g,(25.10±4.90)분,(6.60±1.20)분,]균명현고우모형조[분별위(77.15±14.66)g,(40.75±6.05)g,(18.20±2.00)분,(3.20±0.60)분],강박유영불동시간(117.30±7.22)s칙단우모형조(148.10±8.25)s,차이유현저성(P치분별위0.0425,0.0087,0.0409,0.0317,0.0025);아위산납치료환능부분역전응격인기적동물해마구신경원경도수종、변성.결론 아위산납대만성응격대서억욱모형유명현적항억욱작용.
Objective To measure the antidepressive activity of sodium ferulate (SF) in chronic unpre-dictable mild stress (CUMS) rat model of depression. Methods Male Sprague-Dawley (SD) strain rats weighing 220～280g were divided into six matched groups (n=11 in each group ) based on their sucrose consumption in preliminary tests (1% sucrose solution) : control, CUMS(model) , CUMS + fluoxetine (FLU) ( positive control), and treatment ( CUMS + SF) groups. The rats in model group were exposed to CUMS for 28 days;the rats in posi-ly) for 28 days;the animals in treatment groups were exposed to CUMS and received administration of SF (20,40, care and received ip administration of normal saline simultaneously. The rats except control were housed separately in different rooms during the duration of the stress procedure. The rat model of depression was established by expo-sing to an unpredictable sequence of stressors;the body weight,food intake and fluid consumption were measured ;the behaviors were examined by open field and forced-swimming tests. A representative sections cutting across hip-pocampal area of the rats in the six groups were examined by light microscopy. Results The rat model of depres-sion has been established successfully at 28th day. The results showed that chronic ip administration of SF signifi-cantly reversed the effects of CUMS on the body weight gain,the consumption of sucrose solution,the squares cross-ing and rearing in open field test and the immobility time during forced-swimming test of the rats [(88.06±7.83) g vs. (77.15±14.66 )g;(66.33±3.03)g vs. (40.75±6.05)g;(25.10±4,90) times vs. (18.20±2.00) times, (6.60±1.20 ) times vs. (3.20±0.60) times ;(117.30±7.22) s vs. (148.10±8.25)s], the values of P were 0.0425,0.0087,0.0409,0.0317,0.0025 respectively,and also partially reversed the effects of CUMS on his-topathology of hippoeampus. Conclusion SF possesses potent antidepressant-like activity,and the antidepressant-like action may result from its neuroprotective effect.