CAJ | 학술논문

目的筛选大鼠颅脑创伤后皮层差异表达的miRNA,为颅脑创伤治疗中的miRNA干预策略提供可供选择的目标miRNA.方法利用miRNA芯片检测伤后6 h、24 h、48 h、72 h组各2例创伤大脑皮层组织与2例正常对照的差异表达基因.并随机挑选差异表达基因做PCR验证.结果创伤后6 h组共有136个miRNA表达,其中2倍以上差异表达上调的13个,2倍以上差异表达下调的14个;24 h组共有118个miRNA表达,其中2倍以上差异表达上调的4个,2倍以上差异表达下调的23个;48 h组共有149个miRNA表达,其中2倍以上差异表达上调的16个,2倍以上差异表达下调的11个;72 h组共有203个miRNA表达,其中2倍以上差异表达上调的19个,2倍以上差异表达下调的5个.其中只有miR-21在4个伤后时间点上均高表达,分别为2.0215、2.0401、2.0702、2.7910.定量PCR验证结果提示芯片结果可信.结论创伤大脑皮层的差异表达miRNA在以往文献中鲜见报道,对其干预有望成为颅脑创伤基因治疗的新靶向.
목적 사선대서로뇌창상후피층차이표체적miRNA,위로뇌창상치료중적miRNA간예책략제공가공선택적목표miRNA.방법 이용miRNA심편검측상후6 h、24 h、48 h、72 h조각2례창상대뇌피층조직여2례정상대조적차이표체기인.병수궤도선차이표체기인주PCR험증.결과 창상후6 h조공유136개miRNA표체,기중2배이상차이표체상조적13개,2배이상차이표체하조적14개;24 h조공유118개miRNA표체,기중2배이상차이표체상조적4개,2배이상차이표체하조적23개;48 h조공유149개miRNA표체,기중2배이상차이표체상조적16개,2배이상차이표체하조적11개;72 h조공유203개miRNA표체,기중2배이상차이표체상조적19개,2배이상차이표체하조적5개.기중지유miR-21재4개상후시간점상균고표체,분별위2.0215、2.0401、2.0702、2.7910.정량PCR험증결과제시심편결과가신.결론 창상대뇌피층적차이표체miRNA재이왕문헌중선견보도,대기간예유망성위로뇌창상기인치료적신파향.
Objective This study was designed to screen the different expression of miRNA after traumatic brain injury (TBI) in rats, and to provide the target miRNA for miRNA interference in TBI treatments.Methods Differently expressd genes were examined using miRNA chip in contused cerebral tissue 6 hrs, 24 hrs, 48 hrs and 72 hrs after TBI (2 cases per time point).The genes that expressed differently were chose randomly for further validation by quantitative PCR. Result 136 different expression of miRNA(13 up-regulated and 14 down-regulated more than twice)were found 6 hrs after injury,149(16 up-regulated and 11 down-regulated more than twice) 48 hrs after injury,and 203 (19 up-regulated and 5 down-regulated) 72 hrs after injury. Only the miR-21 expressed higher at all the four time points (2.0215,2.0401,2.0702 and 2.7910 respectively). Quantitative PCR approved the results as well. Conclusions The interference of differently expressed miRNA after TBI would be a new gene therapy on TBI.