CAJ | 학술논문

目的探讨原癌基因Wip1在乳腺癌组织中的表达及其临床意义.方法应用逆转录聚合酶链反应(RT-PCR)、免疫组织化学、Western印迹方法,检测70例乳腺癌组织、癌旁组织、20例正常乳腺组织中Wip1 mRNA和蛋白表达情况,同时对Wip1高表达与临床病理因素的关系进行了比较研究.结果 RT-PCR:乳腺癌组织、癌旁组织、正常乳腺组织Wip1 mRNA的基因表达值分别为:0.715±0.087、0.175±0.021、0.154±0.022.乳腺癌组织比癌旁组织、正常乳腺组织明显升高(P<0.01).免疫组织化学:3种组织中Wip1蛋白高表达率分别为62.9%(44/70)、2.9%(2/70)、0(0/20).乳腺癌组织比癌旁组织、正常乳腺组织明显升高(P<0.01).Western印迹:3种组织Wip1蛋白相对含量分别为0.688±0.151、0.251±0.043、0.234±0.044.乳腺癌组织比癌旁组织、正常乳腺组织明显升高(P<0.01).Wip1高表达与肿瘤大小、年龄、TNM分期、腋窝淋巴结转移、雌激素受体(ER)、孕激素受体(PR)表达无关,与p53表达呈负相关.结论 Wip1 mRNA和蛋白在乳腺癌组织中高表达,促进乳腺癌的发生和发展.Wip1可能成为乳腺癌基因治疗新的靶点.
목적 탐토원암기인Wip1재유선암조직중적표체급기림상의의.방법 응용역전록취합매련반응(RT-PCR)、면역조직화학、Western인적방법,검측70례유선암조직、암방조직、20례정상유선조직중Wip1 mRNA화단백표체정황,동시대Wip1고표체여림상병리인소적관계진행료비교연구.결과 RT-PCR:유선암조직、암방조직、정상유선조직Wip1 mRNA적기인표체치분별위:0.715±0.087、0.175±0.021、0.154±0.022.유선암조직비암방조직、정상유선조직명현승고(P<0.01).면역조직화학:3충조직중Wip1단백고표체솔분별위62.9%(44/70)、2.9%(2/70)、0(0/20).유선암조직비암방조직、정상유선조직명현승고(P<0.01).Western인적:3충조직Wip1단백상대함량분별위0.688±0.151、0.251±0.043、0.234±0.044.유선암조직비암방조직、정상유선조직명현승고(P<0.01).Wip1고표체여종류대소、년령、TNM분기、액와림파결전이、자격소수체(ER)、잉격소수체(PR)표체무관,여p53표체정부상관.결론 Wip1 mRNA화단백재유선암조직중고표체,촉진유선암적발생화발전.Wip1가능성위유선암기인치료신적파점.
Objective To investigate the expression of proto-oneogene Wip1 in breast cancer tissue and its clinical significance. Methods Through the uses of semi-RT-PCR, immunohistochemieal technique and Western blot, the specimens from 70 patients of breast cancer and 20 normal controls were detected for Wip1 mRNA and protein expression. At the same time, the authors analyzed the relations between the expression of Wip1 in human breast cancer and different clinical pathologic parameters. Results RT-PCR:The values of gene expression of Wip1 mRNA in breast cancer tissue, perieaneerous tissue and normal breast tissue were 0. 715±0. 087, 0. 175± 0. 021 and 0. 154±0. 022 respectively. Thus the value of gene expression in breast cancer tissue was significantly higher than that in perieaneerous tissue or normal breast tissue (P<0. 01 ). Immunohistochemistry:The high expression rates of Wip1 protein in breast cancer tissue, perieancerous tissue and normal breast tissue were 62. 9% (44/70), 2. 9% (2/70) and 0(0/20)respectively and there was a significant difference among these three different tissues ( P<0. 01 ). Western blot:The relative contents of Wip1 protein in breast cancer tissue, perieaneerous tissue and normal breast tissue were 0. 688±0. 151, 0. 251±0. 043 and 0. 234±0. 044 respectively. The relative content of Wip1 protein in breast cancer tissue was significantly higher than that in pericaneerous tissue or normal breast tissue (P <0. 01 ). The high expression of Wip1 protein was negatively correlated with the expression of p53, but it had nothing to do with tumor size, age, tumor staging, axillary lymph node metastasis and expressions of ER and PR. Conclusion The high expression of Wip1 mRNA and its protein in breast cancer tissue may promote the growth of breast cancer. Wip1 may become a new target for therapy of breast cancer.