CAJ | 학술논문

通过根癌农杆菌介导手段，将来自发根农杆菌的rolC基因转化到烟草(Nicotiana tabacum)植株的基因组，并借助GUS组织化学法，PCR扩增法和Southern杂交进行了鉴定证实。 rolC基因改变转基因烟草植株某些性状的特点，如植株高度变矮、花冠变小、雄蕊变短等，还发现rolC基因具有延迟转基因烟草植株花期的作用。
통과근암농간균개도수단，장래자발근농간균적rolC기인전화도연초(Nicotiana tabacum)식주적기인조，병차조GUS조직화학법，PCR확증법화Southern잡교진행료감정증실。 rolC기인개변전기인연초식주모사성상적특점，여식주고도변왜、화관변소、웅예변단등，환발현rolC기인구유연지전기인연초식주화기적작용。
The rolC gene cloned from Agrobacterium rhizo genes was inserted into the genome of tobacco plant using Agrobacterium-media ted transformation method,and the result was verified by GUS analysis,PCR am plification and Southern hybridization.Compared to the control,the transgenic to bacco plants showed significantly dwarfer plant height,later bloom period,smaller flower and shorter androecium.