CAJ | 학술논문

目的研究乙肝病毒在乙肝患者垂直传递中的情况.方法运用分子遗传学的技术方法:单核苷酸多态性(SNP)及多聚酶链反应-单链构相多态性(PCR-SSCP),对30个家庭(68个病例)的乙肝患者及其子女进行了研究.结果在乙肝患者及其受感染后出生子女中游离型及整合型乙肝病毒持续增高,与感染前所生子女间比较差异显著(P＜0.05),SNP分析结果显示在乙肝病毒基因组中,U5序列和非U5序列中许多基因位点发生了碱基替换,插入或缺失,男性乙肝患者及其受感染子女的SNP分析结果被确定.结论乙肝病毒可通过男性患者传递至子女;至此,又一个分子遗传学证据证实了了乙肝病毒的遗传性传递.
목적 연구을간병독재을간환자수직전체중적정황.방법 운용분자유전학적기술방법:단핵감산다태성(SNP)급다취매련반응-단련구상다태성(PCR-SSCP),대30개가정(68개병례)적을간환자급기자녀진행료연구.결과 재을간환자급기수감염후출생자녀중유리형급정합형을간병독지속증고,여감염전소생자녀간비교차이현저(P＜0.05),SNP분석결과현시재을간병독기인조중,U5서렬화비U5서렬중허다기인위점발생료감기체환,삽입혹결실,남성을간환자급기수감염자녀적SNP분석결과피학정.결론 을간병독가통과남성환자전체지자녀;지차,우일개분자유전학증거증실료료을간병독적유전성전체.
Objective: To study vertical transmission of hepatitis B between the hepatitis B patients and their children. Methods: 30 families (68 cases) of hepatitis B patients and their children were examined by the molecular genetic methods and techniques of single nucleotide polymorphism(SNP) and polymerase chain reaction -single strand configuration polymorphism( PCR- SSCP). Results: The frequencies of freed and integrated HBV DNA were higher consistently between the hepatitis B patients and their children born after the infection of hepatitis B, and the difference was more significance than the children born before their parent suffered from hepatitis B, ( P ＜ 0.05 ). SNP of hepatitis B patients and their children was ananlysed and it was discovered that there were base substitution, insertion or deletion in the many base site of HBV genome U5 sequence and non - U5 sequence; the SNP of male hepatitis B patients and their children born after the occurrence of hepatitis B was identical. Conclusion: Hepatitis B may be inherited from the male hepatitis B patients to their children born after the occurrence of hepatitis B; thereby, another molecular genetic evidence is provided for hereditary transmission of hepatitis B.