CAJ | 학술논문

目的将磷酸氯喹作用于白血病细胞株U937,观察其对白血病细胞凋亡的影响;并研究磷酸氯喹是否通过逆转凋亡相关基因PNAS-2蛋白在白血病细胞中异常的亚细胞定位,从而促进细胞凋亡的机制.方法将不同剂量的磷酸氯喹加入体外培养至对数生长期的白血病细胞株U937培养液中.MTT法检测细胞增殖;流式细胞仪和激光共聚焦显微镜检测细胞凋亡,同时观察磷酸氯喹对U937细胞中PNAS-2蛋白亚细胞定位的影响.结果 50 μg/mL磷酸氯喹可显著促进U937细胞发生凋亡,并使白血病细胞中PNAS-2蛋白的异常亚细胞定位发生改变.结论磷酸氯喹对白血病细胞株U937有促进凋亡的作用,其凋亡机制可能是使PNAS-2蛋白在白血病细胞中异常的亚细胞定位恢复正常,对于临床治疗白血病的具有潜在的应用价值.
목적 장린산록규작용우백혈병세포주U937,관찰기대백혈병세포조망적영향;병연구린산록규시부통과역전조망상관기인PNAS-2단백재백혈병세포중이상적아세포정위,종이촉진세포조망적궤제.방법 장불동제량적린산록규가입체외배양지대수생장기적백혈병세포주U937배양액중.MTT법검측세포증식;류식세포의화격광공취초현미경검측세포조망,동시관찰린산록규대U937세포중PNAS-2단백아세포정위적영향.결과 50 μg/mL린산록규가현저촉진U937세포발생조망,병사백혈병세포중PNAS-2단백적이상아세포정위발생개변.결론 린산록규대백혈병세포주U937유촉진조망적작용,기조망궤제가능시사PNAS-2단백재백혈병세포중이상적아세포정위회복정상,대우림상치료백혈병적구유잠재적응용개치.
Objective To observe the effects of chloroquine phosphate on apoptosis of leukemic cell line U937, and investigate whether chloroquine phosphate induces leukemic cell apoptosis by normalizing protein PNAS-2's abnormal subcellular location. Methods Chloroquine phosphate of different concentrations were added into culture fluid of leukemic cell line U937 at logarithmic phase. MTr was used to measure cell proliferation, flow cytometry and laser confocal microscopy were applied to detect cell apoptosis, and immunofluorescence technology was employed to observe the effects of chloroquine phosphate on the changes of subcellular location of protein PNAS-2. Results Apoptosis of leukemic cell line U937 was significantly induced by 50 μg/mL chloroquine phosphate, and subcellular location of protein PNAS-2 was changed. Conclusion Chlorequine phosphate can induce apoptosis of leukemic cell line U937, and the mechanism may be related to the normalization of PNAS-2's abnormal subcellular location in U937 cell line. Chloroquine phosphate has the potential to be used in leukemic therapy.