蚕业科学
蠶業科學
잠업과학
ACTA SERICOLOGICA SINICA
2009年
4期
847-850
,共4页
王海玲%池旭娟%阚雪芹%方向明%刘艳艳%谈建中
王海玲%池旭娟%闞雪芹%方嚮明%劉豔豔%談建中
왕해령%지욱연%감설근%방향명%류염염%담건중
桑椹%桑叶%双向电泳%蛋白质样品
桑椹%桑葉%雙嚮電泳%蛋白質樣品
상심%상협%쌍향전영%단백질양품
Mulberry fruit%Mulberry leaf%Two-dimensional electrophoresis (2-DE)%Protein sample preparation
为建立适合于桑树蛋白质组研究的双向电泳技术体系,以桑品种"大10"的桑椹与叶片为材料,探讨了桑树蛋白质不同提取方法对凝胶电泳效果的影响.结果表明,改良酚提取法与常规的SDS提取法、尿素/硫脲提取法相比,电泳图谱清晰,分离效果良好,能检测到更多的蛋白质组分,适用于桑椹与叶片蛋白质IPG-IEF/SDS-PAGE双向电泳及SDS-PAGE电泳的样品制备.
為建立適閤于桑樹蛋白質組研究的雙嚮電泳技術體繫,以桑品種"大10"的桑椹與葉片為材料,探討瞭桑樹蛋白質不同提取方法對凝膠電泳效果的影響.結果錶明,改良酚提取法與常規的SDS提取法、尿素/硫脲提取法相比,電泳圖譜清晰,分離效果良好,能檢測到更多的蛋白質組分,適用于桑椹與葉片蛋白質IPG-IEF/SDS-PAGE雙嚮電泳及SDS-PAGE電泳的樣品製備.
위건립괄합우상수단백질조연구적쌍향전영기술체계,이상품충"대10"적상심여협편위재료,탐토료상수단백질불동제취방법대응효전영효과적영향.결과표명,개량분제취법여상규적SDS제취법、뇨소/류뇨제취법상비,전영도보청석,분리효과량호,능검측도경다적단백질조분,괄용우상심여협편단백질IPG-IEF/SDS-PAGE쌍향전영급SDS-PAGE전영적양품제비.
In order to develop two-dimensional electrophoresis technology suitable for mulberry proteome analysis, the effects of various protein extraction methods on gel electrophoresis were investigated by using fruit and leaves of a mulberry variety named "Da 10". The results showed that the modified phenol extraction method could yield clearer electrophoretogram and better separation effect over the conventional SDS extraction method and urea/thiourea extraction method. With the modified phenol method, more protein components could be detected, being a suitable method for protein sample preparation from mulberry fruit and leaf for two-dimensional IPG-IEF/SDS-PAGE and SDS-PAGE.
