CAJ | 학술논문

目的比较戊型肝炎病毒总抗体(HEV-Ab)与IgG、IgM单抗体检测结果的一致性.方法采用HEV-Ab、HEV-IgG、HEV-IgM 3种试剂盒分别对2070份献血者标本进行检测.总抗体阳性、IgG及IgM中至少有1种阳性,或总抗体阴性、IgG及IgM均阴性判为一致.结果总抗体HEV-Ab检测与IgG及IgM检测的一致性为96.9%(2006/2070);HEV-Ab总抗体S/CO值越高,阳性结果的一致性越好.HEV-Ab S/CO值≥5、2≤S/CO<5和1≤S/CO<2时,检测结果的一致率分别为98.2%(493/502)、79.3%(46/58)和59.5%(22/37).结论戊型肝炎病毒总抗体试剂盒(HEV-Ab)与IgG、IgM单抗体试剂盒(HEV-IgG、HEV-IgM)一致性好,HEV-Ab可用于HEV流行病学研究.
목적 비교무형간염병독총항체(HEV-Ab)여IgG、IgM단항체검측결과적일치성.방법 채용HEV-Ab、HEV-IgG、HEV-IgM 3충시제합분별대2070빈헌혈자표본진행검측.총항체양성、IgG급IgM중지소유1충양성,혹총항체음성、IgG급IgM균음성판위일치.결과 총항체HEV-Ab검측여IgG급IgM검측적일치성위96.9%(2006/2070);HEV-Ab총항체S/CO치월고,양성결과적일치성월호.HEV-Ab S/CO치≥5、2≤S/CO<5화1≤S/CO<2시,검측결과적일치솔분별위98.2%(493/502)、79.3%(46/58)화59.5%(22/37).결론 무형간염병독총항체시제합(HEV-Ab)여IgG、IgM단항체시제합(HEV-IgG、HEV-IgM)일치성호,HEV-Ab가용우HEV류행병학연구.
Objective To evaluate the consistency between the total anti-HEV IgG/IgM assay(HEV-Ab)and the separated anti-HEV IgG(HEV-IgG)and anti-HEV IgM(HEV-IgM)assays.Methods 2070 plasma samples of blood donors were tested for anti-HEV IgG,anti-HEV IgM,and total anti-HEV IgG/IgM by HEV-IgG,HEV-IgM and HEV-Ab EIA assay kits(Beijing Wanti).HEV-Ab positive result with either one or both HEV-IgG and HEV-IgM positive results or HEV-Ab negative sesult with both HEV-IgG and HEV-IgM negative results were considered as consistent.Results The consistency between the HEV-Ab assay and the separated HEV-IgG and HEV-IgM assays was 96.9%(2006/2070).With the increase of the HEV-Ab's signal to cut off ratios(S/CO),the consistent rates were increased.With the HEV-Ab S/CO≥5,2≤S/CO＜5 and 1≤S/CO＜2,the consistent rate was 98.2%(493/502),79.3%(46/58)and 59.5%(22/37),respectively.Conclusions Our study has shown a good consistency between the HEV-Ab assay and the separated HEV-IgG and HEV-IgM assays.HEV-Ab assay can be used for HEV epidemiological study.