CAJ | 학술논문

利用PCR-SSCP技术对高繁殖力绵羊品种湖羊雌激素受体(estrogen receptor,ESR)基因第一外显子的部分序列进行单核甘酸多态性研究.结果表明,湖羊ESR基因中存在3种基因型AA,BB,AB,且A等位基因频率为0.554,B 等位基因频率为0.446.经测序,BB型与AA型相比在外显子1第363位发生1处C→G的碱基突变.据产羔数统计,AB基因型和BB基因型的湖羊产羔数分别比 AA 基因型多0.98只(P<0.01)、1.47只(P<0.01).说明ESR基因可能是控制湖羊多羔性能的一个主效基因或与之存在紧密遗传连锁的一个标记.
이용PCR-SSCP기술대고번식력면양품충호양자격소수체(estrogen receptor,ESR)기인제일외현자적부분서렬진행단핵감산다태성연구.결과표명,호양ESR기인중존재3충기인형AA,BB,AB,차A등위기인빈솔위0.554,B 등위기인빈솔위0.446.경측서,BB형여AA형상비재외현자1제363위발생1처C→G적감기돌변.거산고수통계,AB기인형화BB기인형적호양산고수분별비 AA 기인형다0.98지(P<0.01)、1.47지(P<0.01).설명ESR기인가능시공제호양다고성능적일개주효기인혹여지존재긴밀유전련쇄적일개표기.
Single nucleotide polymorphism in exon 1 of the estrogen receptor (ESR) gene was detected by PCR-SSCP in high fecundity Hu sheep breed. The results indicated that there were three genotypes (AA,BB,AB) in Hu sheep and the frequency of allele A and B was 0.554 and 0.446,respectively. Comparison with the AA genotype, sequencing revealed a C→G mutation at 363 bp of exon 1 of ESR gene in the BB genotype. The Hu sheep with AB genotype or BB genotype had 0.98 and 1.47 more Iambs than those with AA genotype,respectively. These results showed that the estrogen receptor locus was a major gene that controlled the prolificacy in Hu sheep or a mark in close linkage with such a gene.