CAJ | 학술논문

目的:观察老龄大鼠的脑组织内不同部位的葡萄糖转运蛋白3(GLUT 3)的表达情况,探讨GLUT 3在神经系统衰老过程中的作用.方法:用免疫组织化学染色方法,测定3月龄、18月龄及30月龄大鼠(各10只)脑组织内不同部位GLUT 3的表达强弱.结果:各月龄组大鼠脑组织内不同部位阳性细胞数均有显著差异(P<0.01),其中海马区,尤其是海马CA1 区阳性细胞数最多,而运动皮质区及第1小脑小叶阳性细胞数相对较少.结论:在老龄大鼠脑组织中GLUT 3的表达减弱,提示GLUT 3在神经系统衰老过程中发挥一定作用.
목적:관찰노령대서적뇌조직내불동부위적포도당전운단백3(GLUT 3)적표체정황,탐토GLUT 3재신경계통쇠로과정중적작용.방법:용면역조직화학염색방법,측정3월령、18월령급30월령대서(각10지)뇌조직내불동부위GLUT 3적표체강약.결과:각월령조대서뇌조직내불동부위양성세포수균유현저차이(P<0.01),기중해마구,우기시해마CA1 구양성세포수최다,이운동피질구급제1소뇌소협양성세포수상대교소.결론:재노령대서뇌조직중GLUT 3적표체감약,제시GLUT 3재신경계통쇠로과정중발휘일정작용.
Objective: To examine the distribution of glucose transport 3 (GLUT 3) in different brain regions of aged rats and to investigate its role in ageing process of the nervous system. Methods:The GLUT 3 expression in different brain regions was examined with immunohistochemical method in rats aged 3,18 and 30 months, respectively. Results: The number of GLUT 3-positive cells varied in the different brain regions in rats of all age groups (P<0.01); the CA1 region contained the greatest number of positive cells,and fewer in the motor cortex and cerebellum.The number of GLUT 3-positive cells was reduced in the brain of aged rats (P<0.01); and the neural cells in 4 different brain regions presented with large cell body and loose alignment. Conclusion: The expression of GLUT 3 decreased in aged rats,which suggests that GLUT 3 may be involved in the ageing process of nervous system.