中国药理学通报
中國藥理學通報
중국약이학통보
CHINESE PHARMACOLOGICAL BULLETIN
2010年
2期
267-270
,共4页
朱力阳%彭成%谢晓芳%王嫣虹
硃力暘%彭成%謝曉芳%王嫣虹
주력양%팽성%사효방%왕언홍
内脏高敏感性%大鼠模型%肠易激综合征%功能性疾病%冰醋酸%芥末油
內髒高敏感性%大鼠模型%腸易激綜閤徵%功能性疾病%冰醋痠%芥末油
내장고민감성%대서모형%장역격종합정%공능성질병%빙작산%개말유
visceral hypersensitivity%SD rat model%irritable bowel syndrome(IBS)%functional disease%acetic acid%mustard
目的 本文采用两种化学刺激建立两种新的大鼠内脏高敏感性肠易激综合征(IBS)模型.方法 采用成年大鼠,经肠道连续6 d给予冰醋酸或芥末油刺激后,进行直肠扩张,评估肠道敏感性,记录胃肠电频率、峰电位、峰峰值和面积,检测血清5-HT含量.结果 与对照组相比,冰醋酸模型肠敏感性(P<0.05)、胃肠电频率(P<0.01)均升高;芥末油模型肠敏感性(P<0.01),胃肠电频率(P<0.01)、峰电位(P<0.05)、峰峰值(P<0.01)、峰面积(P<0.01),血清5-HT含量(P<0.05)均有变化,提示模型组敏感性增高.与模型组比较,反证药物组肠敏感性、胃肠电及血清5-HT含量均有一定恢复.结论 通过对成年大鼠肠道进行化学刺激,成功建立了新的大鼠内脏高敏感性IBS模型.
目的 本文採用兩種化學刺激建立兩種新的大鼠內髒高敏感性腸易激綜閤徵(IBS)模型.方法 採用成年大鼠,經腸道連續6 d給予冰醋痠或芥末油刺激後,進行直腸擴張,評估腸道敏感性,記錄胃腸電頻率、峰電位、峰峰值和麵積,檢測血清5-HT含量.結果 與對照組相比,冰醋痠模型腸敏感性(P<0.05)、胃腸電頻率(P<0.01)均升高;芥末油模型腸敏感性(P<0.01),胃腸電頻率(P<0.01)、峰電位(P<0.05)、峰峰值(P<0.01)、峰麵積(P<0.01),血清5-HT含量(P<0.05)均有變化,提示模型組敏感性增高.與模型組比較,反證藥物組腸敏感性、胃腸電及血清5-HT含量均有一定恢複.結論 通過對成年大鼠腸道進行化學刺激,成功建立瞭新的大鼠內髒高敏感性IBS模型.
목적 본문채용량충화학자격건립량충신적대서내장고민감성장역격종합정(IBS)모형.방법 채용성년대서,경장도련속6 d급여빙작산혹개말유자격후,진행직장확장,평고장도민감성,기록위장전빈솔、봉전위、봉봉치화면적,검측혈청5-HT함량.결과 여대조조상비,빙작산모형장민감성(P<0.05)、위장전빈솔(P<0.01)균승고;개말유모형장민감성(P<0.01),위장전빈솔(P<0.01)、봉전위(P<0.05)、봉봉치(P<0.01)、봉면적(P<0.01),혈청5-HT함량(P<0.05)균유변화,제시모형조민감성증고.여모형조비교,반증약물조장민감성、위장전급혈청5-HT함량균유일정회복.결론 통과대성년대서장도진행화학자격,성공건립료신적대서내장고민감성IBS모형.
Aim To establish two new rat models of visceral hypersensitivity in IBS by two chemical irritants.Methods Acetic acid or mustard was infused for six days in intestines of adult rats.After modeling,the rectal distention was performed and the thresholds of abdominal withdrawal reflex were measured.The frequency,peak value,peak-nadir value and area of the gastric and enteric electrical activity were recorded.And the contents of 5-HT in the blood serum were detected.Results Compared with the control group,the colon and rectum's sensitivity(P<0.05)and the frequency(P<0.01) of the acetic acid model were heightened.Meanwhile,the colon and rectum's sensitivity(P<0.01),the frequency(P<0.01),peak value(P<0.05),peak-nadir value(P<0.01)and area(P<0.01),and the contents of 5-HT(P<0.05)in serum of the mustard model were all changed,which indicated the increasing of sensitivity of the model.The colon and rectum's sensitivity,the gastric and enteric electrical activity and the contents of 5-HT in serum of the proving group were recovered to some extent.Conclusion The new rat model of visceral hypersensitivity in IBS is successfully set up by stimulating the intestines of adult rats with chemical substances.
