中西医结合学报
中西醫結閤學報
중서의결합학보
JOURNAL OF CHINESE INTEGRATIVE MDEICINE
2012年
10期
1171-1178
,共8页
Veena Sharma%Ritu Paliwal%Pracheta Janmeda%Shatruhan Sharma
Veena Sharma%Ritu Paliwal%Pracheta Janmeda%Shatruhan Sharma
Veena Sharma%Ritu Paliwal%Pracheta Janmeda%Shatruhan Sharma
油椒木%植物提取物%9,10-二甲基-1,2-苯并蒽%致癌物%细胞毒性,免疫%小鼠
油椒木%植物提取物%9,10-二甲基-1,2-苯併蒽%緻癌物%細胞毒性,免疫%小鼠
유초목%식물제취물%9,10-이갑기-1,2-분병은%치암물%세포독성,면역%소서
Moringa oleifera%plant extracts%9,10-dimethyl- 1,2-benzanthracene%carcinogens%cytotoxicity,immunologic%mice
目的:通过二甲基苯蒽(7,12-dimethylbenz[a]anthracene,DMBA)诱导雄性白化小鼠肾组织损伤来研究油椒木豆荚的水乙醇提取物对肾脏的保护作用.方法:予实验小鼠口服15 mg/kg DMBA两周前,分别予200和400 mg/kg的油椒木豆荚的水乙醇提取物以及浓度为0.5％和1％的叔丁基对羟基茴香醚灌胃.测定各组小鼠细胞色素(cytochrome,Cyt)P450及Cytb5、还原型谷光甘肽(reduced glutathione,GSH)、谷胱甘肽转移酶(glutathione-S-transferase,GST)以及肾组织天冬氨酸氨基转移酶( aspartate transaminase,AST)、丙氨酸氨基转移酶(alanine transaminase,ALT)、碱性磷酸酶(alkaline phosphatase,ALP)活性及总胆固醇和蛋白质含量.结果:口服15 mg/kg的DMBA可显著增加小鼠Cyt P450和Cyt b5的浓度(P＜0.01).小鼠肾组织GSH、GST活性显著降低(P＜0.01,P＜0.05).实验小鼠在口服DMBA后,肾组织AST、ALT、ALP活性和蛋白质含量也显著降低,总胆固醇含量则显著增加(P＜0.01).但是经200和400 mg/kg油椒木豆荚提取物预处理14 d的实验小鼠,其由DMBA诱发的各项组织生化指标变化发生显著逆转(P＜0.01).结论:油椒木豆荚的提取物可通过提高细胞抗氧化活性、减少自由基的生成来减少DMBA的毒副作用,起到保护肾组织的作用.
目的:通過二甲基苯蒽(7,12-dimethylbenz[a]anthracene,DMBA)誘導雄性白化小鼠腎組織損傷來研究油椒木豆莢的水乙醇提取物對腎髒的保護作用.方法:予實驗小鼠口服15 mg/kg DMBA兩週前,分彆予200和400 mg/kg的油椒木豆莢的水乙醇提取物以及濃度為0.5％和1％的叔丁基對羥基茴香醚灌胃.測定各組小鼠細胞色素(cytochrome,Cyt)P450及Cytb5、還原型穀光甘肽(reduced glutathione,GSH)、穀胱甘肽轉移酶(glutathione-S-transferase,GST)以及腎組織天鼕氨痠氨基轉移酶( aspartate transaminase,AST)、丙氨痠氨基轉移酶(alanine transaminase,ALT)、堿性燐痠酶(alkaline phosphatase,ALP)活性及總膽固醇和蛋白質含量.結果:口服15 mg/kg的DMBA可顯著增加小鼠Cyt P450和Cyt b5的濃度(P＜0.01).小鼠腎組織GSH、GST活性顯著降低(P＜0.01,P＜0.05).實驗小鼠在口服DMBA後,腎組織AST、ALT、ALP活性和蛋白質含量也顯著降低,總膽固醇含量則顯著增加(P＜0.01).但是經200和400 mg/kg油椒木豆莢提取物預處理14 d的實驗小鼠,其由DMBA誘髮的各項組織生化指標變化髮生顯著逆轉(P＜0.01).結論:油椒木豆莢的提取物可通過提高細胞抗氧化活性、減少自由基的生成來減少DMBA的毒副作用,起到保護腎組織的作用.
목적:통과이갑기분은(7,12-dimethylbenz[a]anthracene,DMBA)유도웅성백화소서신조직손상래연구유초목두협적수을순제취물대신장적보호작용.방법:여실험소서구복15 mg/kg DMBA량주전,분별여200화400 mg/kg적유초목두협적수을순제취물이급농도위0.5％화1％적숙정기대간기회향미관위.측정각조소서세포색소(cytochrome,Cyt)P450급Cytb5、환원형곡광감태(reduced glutathione,GSH)、곡광감태전이매(glutathione-S-transferase,GST)이급신조직천동안산안기전이매( aspartate transaminase,AST)、병안산안기전이매(alanine transaminase,ALT)、감성린산매(alkaline phosphatase,ALP)활성급총담고순화단백질함량.결과:구복15 mg/kg적DMBA가현저증가소서Cyt P450화Cyt b5적농도(P＜0.01).소서신조직GSH、GST활성현저강저(P＜0.01,P＜0.05).실험소서재구복DMBA후,신조직AST、ALT、ALP활성화단백질함량야현저강저,총담고순함량칙현저증가(P＜0.01).단시경200화400 mg/kg유초목두협제취물예처리14 d적실험소서,기유DMBA유발적각항조직생화지표변화발생현저역전(P＜0.01).결론:유초목두협적제취물가통과제고세포항양화활성、감소자유기적생성래감소DMBA적독부작용,기도보호신조직적작용.
OBJECTIVE:To investigate the potential of hydroethanolic extract of Moringa oleifera (MOHE) against 7,12-dimethylbenz[a]anthracene (DMBA)-induced toxicity in male Swiss albino mice.METHODS:Experimental mice were respectively pretreated with 200 and 400 mg/kg of MOHE,and 0.5％ and 1％ of butylated hydroxyanisole (BHA) for two weeks prior to the administration of 15 mg/kg of DMBA,respectively.Levels of xenobiotic metabolizing enzymes such as cytochrome (Cyt) P450 and Cyt b5,activities of reduced glutathione (GSH) and glutathione-S-transferase (GST) and renal aspartate aminotransaminase (AST),alanine aminotransaminase (ALT) and alkaline phosphatase (ALP),and content of protein and total cholesterol were measured to determine the nephrotoxicity caused by DMBA and to elucidate the ameliorating role of M.oleifera.RESULTS:Single oral administration of 15 mg/kg of DMBA resulted in significant increases in Cyt P450 and Cyt b5 (P＜0.01).The toxic effect of DMBA was justified by the significant decreases in the activities of GSH and GST in renal tissues (P＜0.05).The levels of renal AST,ALT and ALP and protein content which are indicative of renocellular damage were also found decreased along with significant increase in total cholesterol content in DMBA-treated mice (P＜0.01).The DMBA-induced alterations in the tissues were significantly reversed after pretreatment with 200 and 400 mg/kg of MOHE orally for 14 d (P＜0.01).CONCLUSION:The effects of MOHE in enhancing the levels of antioxidants and enhancing the levels of biochemical assays in DMBA-induced carcinogenesis are by reducing the formation of free radicals.This study rationalizes the ethnomedicinal use of M.oleifera for the protection against nephrotoxicity induced by chemical carcinogens.
