中华肝脏病杂志
中華肝髒病雜誌
중화간장병잡지
CHINESE JOURNAL OF HEPATOLOGY
2010年
1期
9-12
,共4页
谭行华%李常青%邹尚荣%谢敏%张爱民%李文莉%李小月%黄惠芬%雷春亮
譚行華%李常青%鄒尚榮%謝敏%張愛民%李文莉%李小月%黃惠芬%雷春亮
담행화%리상청%추상영%사민%장애민%리문리%리소월%황혜분%뢰춘량
肝硬化%中草药%基质金属蛋白酶2%二甲基亚硝胺
肝硬化%中草藥%基質金屬蛋白酶2%二甲基亞硝胺
간경화%중초약%기질금속단백매2%이갑기아초알
Liver cirrhosis%Drugs,Chinese herbal%Matrix metalloproteinase-2%Dimethyl nitrosamine
目的 观察安络化纤丸对二甲基亚硝胺诱导大鼠肝纤维化形成的抑制作用.方法 采用二甲基亚硝胺诱导大鼠肝纤维化模型,观察安络化纤丸干预后大鼠肝功能、血清透明质酸(HA)和肝组织羟脯胺酸含量变化,并观察肝组织病理学改变和基质金属蛋白酶2(MMP-2)的表达情况.多组计量资料分析采用One way ANOVA,多重比较采用LSD方法.结果 安络化纤丸干预组血清ALT、AST水平分别为(129.08±53.45)U/L和(321.25±138.32)U/L,血清HA和肝组织羟脯胺酸含量分别为(1644.47±380.45)ng/ml和(0.23±0.08)μg/mg,均明显低于模型组[分别为(611.77±354.17)U/L,(1199.00±763.54)U/L,(3768.38±851.98)ng/ml,(0.51±0.13)μg/mg],差异均具有统计学意义(P<0.01).光镜下显示安络化纤丸干预组大鼠肝组织病理改变较模型组明显减轻(P<0.01),肝组织MMP-2表达强度明显高于模型组(P<0.05).结论 安络化纤丸对二甲基亚硝胺诱导的大鼠肝纤维化形成有较好的抑制作用,其可能的机制与保肝降酶、增强肝组织MMP-2活性和促进细胞外基质降解有关.
目的 觀察安絡化纖汍對二甲基亞硝胺誘導大鼠肝纖維化形成的抑製作用.方法 採用二甲基亞硝胺誘導大鼠肝纖維化模型,觀察安絡化纖汍榦預後大鼠肝功能、血清透明質痠(HA)和肝組織羥脯胺痠含量變化,併觀察肝組織病理學改變和基質金屬蛋白酶2(MMP-2)的錶達情況.多組計量資料分析採用One way ANOVA,多重比較採用LSD方法.結果 安絡化纖汍榦預組血清ALT、AST水平分彆為(129.08±53.45)U/L和(321.25±138.32)U/L,血清HA和肝組織羥脯胺痠含量分彆為(1644.47±380.45)ng/ml和(0.23±0.08)μg/mg,均明顯低于模型組[分彆為(611.77±354.17)U/L,(1199.00±763.54)U/L,(3768.38±851.98)ng/ml,(0.51±0.13)μg/mg],差異均具有統計學意義(P<0.01).光鏡下顯示安絡化纖汍榦預組大鼠肝組織病理改變較模型組明顯減輕(P<0.01),肝組織MMP-2錶達彊度明顯高于模型組(P<0.05).結論 安絡化纖汍對二甲基亞硝胺誘導的大鼠肝纖維化形成有較好的抑製作用,其可能的機製與保肝降酶、增彊肝組織MMP-2活性和促進細胞外基質降解有關.
목적 관찰안락화섬환대이갑기아초알유도대서간섬유화형성적억제작용.방법 채용이갑기아초알유도대서간섬유화모형,관찰안락화섬환간예후대서간공능、혈청투명질산(HA)화간조직간포알산함량변화,병관찰간조직병이학개변화기질금속단백매2(MMP-2)적표체정황.다조계량자료분석채용One way ANOVA,다중비교채용LSD방법.결과 안락화섬환간예조혈청ALT、AST수평분별위(129.08±53.45)U/L화(321.25±138.32)U/L,혈청HA화간조직간포알산함량분별위(1644.47±380.45)ng/ml화(0.23±0.08)μg/mg,균명현저우모형조[분별위(611.77±354.17)U/L,(1199.00±763.54)U/L,(3768.38±851.98)ng/ml,(0.51±0.13)μg/mg],차이균구유통계학의의(P<0.01).광경하현시안락화섬환간예조대서간조직병리개변교모형조명현감경(P<0.01),간조직MMP-2표체강도명현고우모형조(P<0.05).결론 안락화섬환대이갑기아초알유도적대서간섬유화형성유교호적억제작용,기가능적궤제여보간강매、증강간조직MMP-2활성화촉진세포외기질강해유관.
Objective To investigate the effects of anluohuaqianwan on experimental hepatic fibrosis induced by dimethyl nitrosamine (DMN) in rats. Methods 36 male SD rats were randomly dividied into three groups: model group, normal group, anluohuaqianwan group. The rats in the three groups were treated with DMN daily for 4 weeks. The liver function was detected using auto biochemistry analyzer, the serum HA, LN, IV-C, PIIIP were detected by immunoradiometry, the histopathology was observed in the left liver lobe after HE staining, the expression of matrix metalloproteinase-2 (MMP-2) in liver tissue were de-tected by immunohistochemistry. Results The serum levels of ALT, AST, ALP, TP, ALB and the contents of HA, LN, IV-C in model group were significantly increased compared to these in the normal group (P < 0.01). The serum levels of ALT, AST and the contents of HA in anluohuaqianwan group were significantly lower than those in the model group (P < 0.01). The liver MMP-2 in the model group was significantly increased compared to that in the normal group (P < 0.05). The expression of MMP-2 in fiver tissue of model group was lower than that in the anluohuaqianwan group (P < 0.05). Conclusion Anluohuaqianwan can inhibit liver fibrosis in rats induced by DMN.
