中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2011年
2期
144-148
,共5页
唐湘莲%姜明%郑毅雄%孙崇德
唐湘蓮%薑明%鄭毅雄%孫崇德
당상련%강명%정의웅%손숭덕
胃肿瘤%葡萄糖苷类%肿瘤转移%基质金属蛋白酶2
胃腫瘤%葡萄糖苷類%腫瘤轉移%基質金屬蛋白酶2
위종류%포도당감류%종류전이%기질금속단백매2
Stomach neoplasms%Glucosides%Neoplasm metastasis%Matrix metalloproteinase 2
目的 观察矢车菊素-3-葡萄糖苷(C3G)对人胃癌细胞株MGC803和SGC7901增殖、迁移和侵袭运动能力的影响,并初步探讨其可能的机制.方法 用C3G处理人胃癌MGC803和SGC7901细胞株,用MTF比色法检测C3G对该细胞系增殖的抑制作用,Transwe1l小室进行迁移实验和人工重组基底膜侵袭实验.用RT-PCR及Western blot方法检测用药前后肿瘤细胞中基质金属蛋白酶2(MMP-2)基因和蛋白的表达水平.结果 C3G在体外明显抑制人胃癌MGC803和SGC7901细胞的增殖,且呈浓度、时间依赖性(P＜0.01),MGC803 24 hIC50=6.27μg/ml,SCC7901 24 h IC50=5.42 μg/ml.经C3G处理后,MGC803和SGC7901细胞迁移、侵袭能力明显降低(P＜0.01),MGC803和SGC7901阴性对照组24 h侵袭细胞数分别为(207±9)个和(115±9)个,C3G 10 μg/ml组侵袭细胞数则分别减少至(24±5)个和(14±6)个.MMP-2基因和蛋白表达水平显著下降(P＜0.01),且呈现浓度依赖性.结论 C3G具有体外抗胃癌细胞增殖的作用,且可能通过抑制肿瘤细胞的迁移和侵袭运动能力来发挥预防肿瘤侵袭的作用,其机制可能与抑制MMP-2的生成有关.
目的 觀察矢車菊素-3-葡萄糖苷(C3G)對人胃癌細胞株MGC803和SGC7901增殖、遷移和侵襲運動能力的影響,併初步探討其可能的機製.方法 用C3G處理人胃癌MGC803和SGC7901細胞株,用MTF比色法檢測C3G對該細胞繫增殖的抑製作用,Transwe1l小室進行遷移實驗和人工重組基底膜侵襲實驗.用RT-PCR及Western blot方法檢測用藥前後腫瘤細胞中基質金屬蛋白酶2(MMP-2)基因和蛋白的錶達水平.結果 C3G在體外明顯抑製人胃癌MGC803和SGC7901細胞的增殖,且呈濃度、時間依賴性(P＜0.01),MGC803 24 hIC50=6.27μg/ml,SCC7901 24 h IC50=5.42 μg/ml.經C3G處理後,MGC803和SGC7901細胞遷移、侵襲能力明顯降低(P＜0.01),MGC803和SGC7901陰性對照組24 h侵襲細胞數分彆為(207±9)箇和(115±9)箇,C3G 10 μg/ml組侵襲細胞數則分彆減少至(24±5)箇和(14±6)箇.MMP-2基因和蛋白錶達水平顯著下降(P＜0.01),且呈現濃度依賴性.結論 C3G具有體外抗胃癌細胞增殖的作用,且可能通過抑製腫瘤細胞的遷移和侵襲運動能力來髮揮預防腫瘤侵襲的作用,其機製可能與抑製MMP-2的生成有關.
목적 관찰시차국소-3-포도당감(C3G)대인위암세포주MGC803화SGC7901증식、천이화침습운동능력적영향,병초보탐토기가능적궤제.방법 용C3G처리인위암MGC803화SGC7901세포주,용MTF비색법검측C3G대해세포계증식적억제작용,Transwe1l소실진행천이실험화인공중조기저막침습실험.용RT-PCR급Western blot방법검측용약전후종류세포중기질금속단백매2(MMP-2)기인화단백적표체수평.결과 C3G재체외명현억제인위암MGC803화SGC7901세포적증식,차정농도、시간의뢰성(P＜0.01),MGC803 24 hIC50=6.27μg/ml,SCC7901 24 h IC50=5.42 μg/ml.경C3G처리후,MGC803화SGC7901세포천이、침습능력명현강저(P＜0.01),MGC803화SGC7901음성대조조24 h침습세포수분별위(207±9)개화(115±9)개,C3G 10 μg/ml조침습세포수칙분별감소지(24±5)개화(14±6)개.MMP-2기인화단백표체수평현저하강(P＜0.01),차정현농도의뢰성.결론 C3G구유체외항위암세포증식적작용,차가능통과억제종류세포적천이화침습운동능력래발휘예방종류침습적작용,기궤제가능여억제MMP-2적생성유관.
Objective To investigate the effect of cyanidin-3-glucoside extracted from Chinese bayberry on the proliferation. migration and invasion ability of the gastric cancer cell lines MGC803 and SGC7901 in vitro, and explore the possible mechanism of the preventive effects of C3G on tumor metastasis.Methods After treatment by C3G, the growth inhibiting of C3G on MGC803 and SGC7901 was determined by MTF assay, cell migration and invasion ability was evaluated with transwell chamber. Expression of Matrix metalloproteinase 2( MMP-2 )mRNA and protion on cells were analyzed by RT-PCR and Western blot.Results C3G significantly inhibited the proliferation of MGC803 and SGC7901 cells in a concentration and time-dependent manner as measured by MTT method ( P ＜0. 01 ), and the IC50 were: MGC803:24 h IC50 =6. 27 μg/ml;SGC7901:24 h IC50 = 5.42 μg/ml. After the cells were treated with C3G, the migration and invasion ability of MGC803 and SGC7901 cells decreased significantly ( P ＜ 0. 01 ) the number of invasive cells in 24 hours of the negative control MGC803, SGC7901 group was ( 207 ± 9 ) and ( 115 ± 9 ),respectively, while in C3G 10 μg/ml group the number of invasive cells decreased to( 24 ± 5 ) , ( 14 ± 6). In addition, the expression of MMP-2 mRNA and protion decreased abviously ( P ＜ 0. 01 ), all that was in a concentration-dependent manner. Conclusions In vitro, C3G has a concentration- and time-dependent growth inhibiting effect on MGC803 and SGC7901 cells, and may prevent metastasis by affecting migration and invasion ability of tumor cells. This action may be mediated by down-regulation of MMP-2mRNA and protein.
