CAJ | 학술논문

目的评价组织间接免疫荧光检测(IIF)、细胞免疫荧光(CBA)及ELISA 3种不同检测视神经脊髓炎(NMO)特异性抗水通道蛋白4(AQP4)抗体的方法。方法 NMO 29例、多发性硬化(MS)23例、健康志愿者50例，分别采用IIF、CBA及ELISA方法检测血清中的抗AQP4抗体，比较各种方法诊断NMO的敏感性和特异性以及阳性结果的一致性。结果 3种方法检测的抗AQP4抗体诊断NMO的敏感性CBA法(72.4％)＞IIF法(62.1％)＞ELISA法(51.7％)；特异性CBA法(100％)＞ ELISA法(98.6％) ＞IIF法(97.3％)。采用Kappa检验评估各种检测方法一致性，CBA、IIF、ELISA 法均具有较好的一致性，其中CBA法与ELISA法检测一致性最好(P＜0.01)。结论检测抗AQP4 抗体以CBA法敏感性和特异性最佳，CBA与ELISA法阳性结果具有较好的一致性。
목적 평개조직간접면역형광검측(IIF)、세포면역형광(CBA)급ELISA 3충불동검측시신경척수염(NMO)특이성항수통도단백4(AQP4)항체적방법。방법 NMO 29례、다발성경화(MS)23례、건강지원자50례，분별채용IIF、CBA급ELISA방법검측혈청중적항AQP4항체，비교각충방법진단NMO적민감성화특이성이급양성결과적일치성。결과 3충방법검측적항AQP4항체진단NMO적민감성CBA법(72.4％)＞IIF법(62.1％)＞ELISA법(51.7％)；특이성CBA법(100％)＞ ELISA법(98.6％) ＞IIF법(97.3％)。채용Kappa검험평고각충검측방법일치성，CBA、IIF、ELISA 법균구유교호적일치성，기중CBA법여ELISA법검측일치성최호(P＜0.01)。결론 검측항AQP4 항체이CBA법민감성화특이성최가，CBA여ELISA법양성결과구유교호적일치성。
Objective To evaluate three methods of detecting anti-aquaporin 4 (AQP4) antibody in neuromylitis optica (NMO), including indirect immunofluorescence assay organization (IIF), cell immunofluorescence method (CBA) and ELISA.Methods The patients were divided into NMO group (n =29), multiple sclerosis (MS) group (n = 23),and healthy controls group (n = 50).IIF, CBA and ELISA were used in 3 groups to detect serum anti-AQP4 antibody.The sensitivity and specificity as well as the consistency of positive results were compared.Results In the aspect of the sensitivity of the three antiAQP4 antibody to diagnosis NMO, CBA (72.4％) ＞ IIF (62.1％) ＞ ELISA (51.7％) ; in the aspect of specificity, CBA (100.0％) ＞ ELISA (98.6％) ＞ IIF (97.3％).Kappa testing and evaluation method showed that the three detection methods were all in good consistency, particular in CBA and ELISA (P ＜0.01).Conclusions CBA method showed a highest specificity and sensitivity in all these three anti-AQP4 antibody detection methods.CBA and ELISA are in better consistency of positive results.